2003
Intracellular pH Activates Membrane-Bound Na+/H+ Exchanger and Vacuolar H+-ATPase in Human Embryonic Kidney (HEK) Cells
Lang K, Wagner C, Haddad G, Burnekova O, Geibel J. Intracellular pH Activates Membrane-Bound Na+/H+ Exchanger and Vacuolar H+-ATPase in Human Embryonic Kidney (HEK) Cells. Cellular Physiology And Biochemistry 2003, 13: 257-262. PMID: 14586169, DOI: 10.1159/000074540.Peer-Reviewed Original ResearchConceptsMicroM EIPAIntracellular acidificationFree bath solutionProximal tubule cellsInfluence of intracellularWestern blot analysisTime-dependent mannerTubule cellsHuman embryonic kidney cellsHEK-293 cellsExtruding mechanismHuman embryonic kidney cell line HEK-293NHE3 proteinEmbryonic kidney cellsCell line HEK-293Cytosolic pHKidney cellsBlot analysisAbsence of bicarbonateHEK293 cellsBath solutionEIPAPresent studyActivationCells
1990
Amiloride analogs induce the phosphorylation of elongation factor-2 in vascular endothelial cells.
Demolle D, Lecomte M, Boutherin-Falson O, Cragoe E, Nairn A, Boeynaems J. Amiloride analogs induce the phosphorylation of elongation factor-2 in vascular endothelial cells. Molecular Pharmacology 1990, 37: 827-832. PMID: 2359404, DOI: 10.1016/s0026-895x(25)11052-3.Peer-Reviewed Original ResearchConceptsElongation factor 2Protein synthesisFactor 2Rabbit reticulocyte lysateCell-free systemBovine aortic endothelial cellsDependent phosphorylationReticulocyte lysateEndothelial cellsAmiloride analoguesPhosphorylationSimilar MrCytosolic pHVascular endothelial cellsProteinAnalogues of amilorideAortic endothelial cellsPotent inhibitorInhibitory effectAntiportCellsEIPAAmilorideATPLysates
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