2021
Cryo‐EM Structures of Outer‐arm Dynein Array Bound to Microtubule Doublet Reveal a Mechanism for Motor Coordination
Rao Q, Wang Y, Chai P, Kuo Y, Han L, Yang R, Yang Y, Howard J, Zhang K. Cryo‐EM Structures of Outer‐arm Dynein Array Bound to Microtubule Doublet Reveal a Mechanism for Motor Coordination. The FASEB Journal 2021, 35 DOI: 10.1096/fasebj.2021.35.s1.03099.Peer-Reviewed Original ResearchOuter arm dyneinMicrotubule-bound stateInner arm dyneinsCentral pair complexMicrotubule-binding domainMicrotubule doubletsIntermediate chainATP hydrolysisFundamental cellular processesHeavy chainCryo-EM structureCryo-EM analysisCryo-electron tomographyKey motor proteinCryo-electron microscopyLight chainCellular processesEukaryotic ciliaT. thermophilaEmbryonic developmentAdjacent microtubule doubletsCellular motilityMotor proteinsAxonemal dyneinsMotile ciliaStructural insights into the cause of human RSPH4A primary ciliary dyskinesia
Zhao Y, Pinskey J, Lin J, Yin W, Sears P, Daniels L, Zariwala M, Knowles M, Ostrowski L, Nicastro D. Structural insights into the cause of human RSPH4A primary ciliary dyskinesia. Molecular Biology Of The Cell 2021, 32: 1202-1209. PMID: 33852348, PMCID: PMC8351563, DOI: 10.1091/mbc.e20-12-0806.Peer-Reviewed Original ResearchConceptsStructural basisCryo-electron tomographyRadial spokesCentral pair complexUnderlying structural basisAxonemal repeatEukaryotic organellesArch domainThree-dimensional structureSubtomogram averagingOrgan positioningCell motilityStructural insightsPrimary ciliary dyskinesiaCiliaHuman ciliaHuman respiratory ciliaRS1Primary defectStructure determinationCiliary dyskinesiaHuman healthOrganellesFlagellaRepeats
2012
Conserved structural motifs in the central pair complex of eukaryotic flagella
Carbajal‐González B, Heuser T, Fu X, Lin J, Smith BW, Mitchell DR, Nicastro D. Conserved structural motifs in the central pair complex of eukaryotic flagella. Cytoskeleton 2012, 70: 101-120. PMID: 23281266, PMCID: PMC3914236, DOI: 10.1002/cm.21094.Peer-Reviewed Original ResearchConceptsCentral pair complexCilia/flagellaRemarkable structural conservationGreen alga ChlamydomonasConserved Structural MotifCryo-electron tomographyOrganism-specific differencesEukaryotic cellsSea urchin StrongylocentrotusEvolutionary distanceStructural conservationAlga ChlamydomonasThree-dimensional structureSubtomogram averagingEukaryotic flagellaAxonemal dyneinsPrecise regulationInner proteinsMotility regulatorHair-like appendagesSinglet microtubulesChlamydomonasHuman diseasesDoublet microtubulesFlagella
1994
The Chlamydomonas FLA10 gene encodes a novel kinesin-homologous protein.
Walther Z, Vashishtha M, Hall JL. The Chlamydomonas FLA10 gene encodes a novel kinesin-homologous protein. Journal Of Cell Biology 1994, 126: 175-188. PMID: 8027176, PMCID: PMC2120091, DOI: 10.1083/jcb.126.1.175.Peer-Reviewed Original ResearchMeSH KeywordsAlgal ProteinsAmino Acid SequenceAnimalsBase SequenceCell CycleChlamydomonas reinhardtiiCloning, MolecularFlagellaGenes, ProtozoanKinesinsMicrotubule-Associated ProteinsMolecular Sequence DataMorphogenesisProtozoan ProteinsRestriction MappingRNA, MessengerSequence Analysis, DNASequence Homology, Amino AcidConceptsFlagellar assemblyAmino-terminal motor domainUni linkage groupCarboxy-terminal tailKinesin heavy chainTemperature-sensitive defectKinesin-related proteinCentral pair complexMotility mutantsMouse geneLinkage groupsCell divisionFla genesGene productsGenomic DNAFlagellar apparatusNovel roleWestern blot analysisGenesSynchronized culturesNucleotide sequencingAMP-PNPMolecular analysisAxonemeFLA10
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