2012
Identification of Core DNA Elements That Target Somatic Hypermutation
Kohler KM, McDonald JJ, Duke JL, Arakawa H, Tan S, Kleinstein SH, Buerstedde JM, Schatz DG. Identification of Core DNA Elements That Target Somatic Hypermutation. The Journal Of Immunology 2012, 189: 5314-5326. PMID: 23087403, PMCID: PMC3664039, DOI: 10.4049/jimmunol.1202082.Peer-Reviewed Original ResearchMeSH Keywords3' Flanking RegionAnimalsB-LymphocytesCells, CulturedChickensChromatin ImmunoprecipitationCytidine DeaminaseDNAEnhancer Elements, GeneticGenes, ImmunoglobulinGenetic LociImmunoassayImmunoglobulin Variable RegionMutationPhosphorylationRNA Polymerase IISerineSomatic Hypermutation, ImmunoglobulinTranscription, GeneticConceptsActivation-induced deaminaseDNA elementsSomatic hypermutationChicken DT40 B cellsIg lociChromatin immunoprecipitation experimentsDT40 B cellsRNA polymerase IISystematic deletion analysisL chain lociNon-Ig genesCore DNA elementSerine 5Epigenetic marksPolymerase IITranscriptional elongationMutational machineryDeletion analysisReporter cassetteImmunoprecipitation experimentsDeoxycytosine residuesIg genesDNA damageChain locusLoci
2008
Pax5 and Linker Histone H1 Coordinate DNA Methylation and Histone Modifications in the 3′ Regulatory Region of the Immunoglobulin Heavy Chain Locus
Giambra V, Volpi S, Emelyanov AV, Pflugh D, Bothwell AL, Norio P, Fan Y, Ju Z, Skoultchi AI, Hardy RR, Frezza D, Birshtein BK. Pax5 and Linker Histone H1 Coordinate DNA Methylation and Histone Modifications in the 3′ Regulatory Region of the Immunoglobulin Heavy Chain Locus. Molecular And Cellular Biology 2008, 28: 6123-6133. PMID: 18644860, PMCID: PMC2547000, DOI: 10.1128/mcb.00233-08.Peer-Reviewed Original ResearchConceptsImmunoglobulin heavy chain locusHeavy chain locusHistone modificationsDNA methylationRegulatory regionsPalindromic regionMurine immunoglobulin heavy chain locusB-cell-specific transcription factor Pax5Chain locusLinker histone H1Multiple DNase IModular patternsTranscription factor Pax5Class switch recombinationMethylation-sensitive restriction sitesActive chromatinHeavy chain allelesHypersensitive sitesHistone H1B cell differentiationDNase ISwitch recombinationPotential insulatorsRestriction sitesHS4
2003
The properties of the single chicken MHC classical class II α chain (B-LA) gene indicate an ancient origin for the DR/E-like isotype of class II molecules
Salomonsen J, Marston D, Avila D, Bumstead N, Johansson B, Juul-Madsen H, Olesen G, Riegert P, Skjødt K, Vainio O, Wiles M, Kaufman J. The properties of the single chicken MHC classical class II α chain (B-LA) gene indicate an ancient origin for the DR/E-like isotype of class II molecules. Immunogenetics 2003, 55: 605-614. PMID: 14608490, DOI: 10.1007/s00251-003-0620-7.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAmino Acid SequenceAnimalsBlotting, SouthernChickensChromosomesCloning, MolecularDNA, ComplementaryExonsFemaleGenes, MHC Class IIHaplotypesHLA-DP AntigensHLA-DQ AntigensHLA-DR AntigensIntronsMaleMicrosatellite RepeatsMolecular Sequence DataPolymerase Chain ReactionSequence Homology, Amino AcidConceptsPeptide-binding residuesIsotypes of class II moleculesSouthern blot analysis of genomic DNALow level of polymorphismAnalysis of genomic DNALevel of polymorphismGenomic DNA sequencesNon-mammalian vertebratesSouthern blot analysisChicken sequencesBack-cross familiesSequence diversitySequence featuresDNA sequencesGenomic DNAMHC lociChain locusChain transcriptsChain geneAmino acidsClass II moleculesBack-crossGenesSequenceCDNA
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