Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells
Witusik M, Piaskowski S, Hulas-Bigoszewska K, Zakrzewska M, Gresner S, Azizi S, Krynska B, Liberski P, Rieske P. Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells. BMC Biotechnology 2008, 8: 56. PMID: 18638414, PMCID: PMC2488339, DOI: 10.1186/1472-6750-8-56.Peer-Reviewed Original ResearchMeSH KeywordsAstrocytesBiomarkersCell Culture TechniquesCell DifferentiationCell LineageCells, CulturedCulture Media, Serum-FreeDNA-Binding ProteinsFibroblast Growth FactorsFibronectinsGlial Fibrillary Acidic ProteinHMGB ProteinsHumansHyaluronan ReceptorsMicrotubule-Associated ProteinsNerve Growth FactorsNerve Tissue ProteinsNeurogliaNeuronsS100 Calcium Binding Protein beta SubunitS100 ProteinsSOXB1 Transcription FactorsStem CellsTranscription FactorsVimentinConceptsNon-neural cellsNormal human astrocytesControlling differentiation of neural stem cellsCell culture conditionsNeuronal cellsCulture conditionsCell culture manipulationsSupplementing serum-free mediumElimination of glial cellsExtracellular factorsDifferentiation of neural stem cellsCell differentiationGlial cellsGrowth factorControl differentiationEnvironmental changesInfluence of intrinsic factorsNeural stem cellsEnvironmental conditionsCell culturesSerum-free mediumNeural progenitorsBackgroundAlthough extensive researchNeural cellsNeural cell cultures
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