Positional-scanning fluorigenic substrate libraries reveal unexpected specificity determinants of DUBs (deubiquitinating enzymes)
Drag M, Mikolajczyk J, Bekes M, Reyes-Turcu FE, Ellman JA, Wilkinson KD, Salvesen GS. Positional-scanning fluorigenic substrate libraries reveal unexpected specificity determinants of DUBs (deubiquitinating enzymes). Biochemical Journal 2008, 415: 367-375. PMID: 18601651, PMCID: PMC2766241, DOI: 10.1042/bj20080779.Peer-Reviewed Original ResearchConceptsMechanism of ubiquitinC-terminal motifUbiquitin-specific proteaseFamily of proteasesP2 positionGroup of proteasesSpecificity determinantsHuman DUBsUCH-L3DUB activityElevated salt concentrationsSubstrate specificitySubstrate librariesCellular poolPositional scanning librariesTetrapeptide substrateUbiquitinAppropriate mutationsNatural substrateDUBsRecognition regionActivation mechanismLys-ArgSubstantial toleranceSmall substrates
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