Estimating the relative rates of lipopolysaccharide synthesis in Escherichia coli K-12 by click chemistry-mediated labeling
Shu S, Mi W. Estimating the relative rates of lipopolysaccharide synthesis in Escherichia coli K-12 by click chemistry-mediated labeling. PLOS ONE 2025, 20: e0325589. PMID: 40549759, PMCID: PMC12184891, DOI: 10.1371/journal.pone.0325589.Peer-Reviewed Original ResearchConceptsSynthesized LPSLabelled lipopolysaccharideE. coli K-12 strain MG1655Escherichia coli K-12Investigating bacterial physiologyPhase of bacterial growthLog phase of bacterial growthIn-gel fluorescenceBacterial membrane integritySynthesis rateNon-radioactive approachHost immune interactionsGram-negative bacteriaLipopolysaccharide synthesisLPS synthesisBacterial physiologySynthesis in vivoLPS biogenesisExponential phaseCopper-free click reactionIn-gelLog phaseMembrane integrityBacterial growthFluorescent alkyne
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