2000
Production of Antibodies That Recognize Specific Tyrosine‐Phosphorylated Peptides
DiGiovanna M, Roussel R, Stern D. Production of Antibodies That Recognize Specific Tyrosine‐Phosphorylated Peptides. Current Protocols In Molecular Biology 2000, 50: 18.6.1-18.6.19. PMID: 18265171, DOI: 10.1002/0471142727.mb1806s50.BooksConceptsAnti-phosphopeptide antibodiesIndividual phosphorylation sitesAnalysis of phosphoproteinsAnti-phosphoamino acid antibodiesPhosphorylation sitesUnphosphorylated proteinTyrosine phosphorylated peptidesCognate proteinPhosphorylated stateParticular proteinAffinity matrixProteinUnique specificityPhosphoproteinFunctional stateDifferential isolationSuch reagentsPhosphotyrosinePeptidesSupport protocolSpeciesAbundanceProduction of antibodiesConventional antibodies
1980
Chemical modification reduces the conductance of sodium channels in nerve
Sigworth F, Spalding B. Chemical modification reduces the conductance of sodium channels in nerve. Nature 1980, 283: 293-295. PMID: 6965422, DOI: 10.1038/283293a0.Peer-Reviewed Original ResearchConceptsChemical modificationTrimethyloxonium ionSuch chemical modificationsCarboxyl groupsNegative chargeSuch reagentsTMO treatmentPermeability propertiesReagentsLinear instantaneous current-voltage relationshipSmaller single-channel conductancePropertiesPotent poisonNormal KdIonsModificationSaxitoxinCharge
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