2004
Solution structure of domain 5 of a group II intron ribozyme reveals a new RNA motif
Sigel RK, Sashital DG, Abramovitz DL, Palmer AG, Butcher SE, Pyle AM. Solution structure of domain 5 of a group II intron ribozyme reveals a new RNA motif. Nature Structural & Molecular Biology 2004, 11: 187-192. PMID: 14745440, DOI: 10.1038/nsmb717.Peer-Reviewed Original ResearchConceptsGroup II intron ribozymeMetal ion titrationsSolution structureMetal ion bindingDivalent metal ionsMetal ion-binding sitesRibozyme active siteBackbone substituentsMetal ionsMajor groove faceIon-binding sitesIon titrationActive siteIon bindingSyn conformationIntron ribozymesMinor groove sideAGC triadNew RNA motifMajor grooveGroove faceStrong Mg2Catalytic triadGroove sideCentral core
1999
An important base triple anchors the substrate helix recognition surface within the Tetrahymena ribozyme active site
Szewczak A, Ortoleva-Donnelly L, Zivarts M, Oyelere A, Kazantsev A, Strobel S. An important base triple anchors the substrate helix recognition surface within the Tetrahymena ribozyme active site. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 11183-11188. PMID: 10500151, PMCID: PMC18008, DOI: 10.1073/pnas.96.20.11183.Peer-Reviewed Original ResearchConceptsHelix dockingBase triplesRecognition surfaceGroup I intronActive siteNetwork of interactionsTetrahymena group IP3 helixStructural biologySubstrate bindingI intronCatalytic RNAProduct bindingSuppression analysisFunctional importanceRNA foldingRNA constructsSubstrate helixBiochemical evidenceMutant ribozymesRibozyme active siteSubstantial rearrangementHelixCrystallographic modelRibozymeA hydrogen-bonding triad stabilizes the chemical transition state of a group I ribozyme
Strobel S, Ortoleva-Donnelly L. A hydrogen-bonding triad stabilizes the chemical transition state of a group I ribozyme. Cell Chemical Biology 1999, 6: 153-165. PMID: 10074469, DOI: 10.1016/s1074-5521(99)89007-3.Peer-Reviewed Original Research
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