2022
A high-throughput assay for directly monitoring nucleolar rRNA biogenesis
Bryant CJ, McCool MA, Abriola L, Surovtseva YV, Baserga SJ. A high-throughput assay for directly monitoring nucleolar rRNA biogenesis. Open Biology 2022, 12: 210305. PMID: 35078352, PMCID: PMC8790372, DOI: 10.1098/rsob.210305.Peer-Reviewed Original ResearchConceptsRibosome biogenesisRRNA biogenesisNucleolar functionSiRNA controlRNA polymerase 1Nucleolar protein fibrillarinPutative small molecule inhibitorsHigh-throughput primary screensHigh-throughput assaysSmall molecule inhibitorsProtein fibrillarinBiogenesisBMH-21Polymerase-1FibrillarinPrimary screenPrimary assayNucleoliAssaysMorphological alterationsRNAP1RibosomopathiesHitsBiological underpinningsDirect quantification
2020
Paired Box 9 (PAX9), the RNA polymerase II transcription factor, regulates human ribosome biogenesis and craniofacial development
Farley-Barnes KI, Deniz E, Overton MM, Khokha MK, Baserga SJ. Paired Box 9 (PAX9), the RNA polymerase II transcription factor, regulates human ribosome biogenesis and craniofacial development. PLOS Genetics 2020, 16: e1008967. PMID: 32813698, PMCID: PMC7437866, DOI: 10.1371/journal.pgen.1008967.Peer-Reviewed Original ResearchConceptsRNA polymerase II transcription factorsCraniofacial developmentTranscription factorsRibosome biogenesis factorsHuman ribosome biogenesisNeural crest developmentBox 9Tissue-specific mannerRibosome biogenesisRibosome productionHuman ribosomopathiesExpression of proteinsPax9 functionBiogenesis factorsXenopus tropicalisCrest developmentSmall subunitEmbryonic developmentCellular machinesUnexpected layerLevels of proteinRibosomopathiesHuman cellsProtein synthesisRibosomes
2016
Expression of ribosomopathy genes during Xenopus tropicalis embryogenesis
Robson A, Owens ND, Baserga SJ, Khokha MK, Griffin JN. Expression of ribosomopathy genes during Xenopus tropicalis embryogenesis. BMC Developmental Biology 2016, 16: 38. PMID: 27784267, PMCID: PMC5081970, DOI: 10.1186/s12861-016-0138-5.Peer-Reviewed Original ResearchConceptsRibosome biogenesis factorsBiogenesis factorsVentral blood islandsHuman congenital diseasesCranial neural crestRNA-seq dataAntisense mRNA probesBlood islandsEmbryonic developmentDistinct tissuesNeural crestProtein productionDifferential expressionTranscript numbersMachinery componentsSpecific tissuesSpecific phenotypesRibosomopathiesSitu hybridizationMRNA probesRibosomesExpressionTissue localizationPhenotypeFactor expressionNop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA
Zhang J, McCann KL, Qiu C, Gonzalez LE, Baserga SJ, Hall TM. Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA. Nature Communications 2016, 7: 13085. PMID: 27725644, PMCID: PMC5062617, DOI: 10.1038/ncomms13085.Peer-Reviewed Original ResearchConceptsEukaryotic ribosome biogenesisCorrect subcellular locationRibosome assembly factorsPre-ribosomal RNAPumilio repeatsRibosome biogenesisHuman ribosomopathiesAssembly factorsBiogenesis factorsRepeat proteinsMature rRNASubcellular locationNop9RNA complexCleavage siteRRNATimely cleavageProteinStructural featuresFinal processing stepRibosomopathiesBiogenesisCleavageYeastNucleaseProbing the mechanisms underlying human diseases in making ribosomes.
Farley KI, Baserga SJ. Probing the mechanisms underlying human diseases in making ribosomes. Biochemical Society Transactions 2016, 44: 1035-44. PMID: 27528749, PMCID: PMC5360156, DOI: 10.1042/bst20160064.Peer-Reviewed Original ResearchThe Contributions of the Ribosome Biogenesis Protein Utp5/WDR43 to Craniofacial Development
Sondalle SB, Baserga SJ, Yelick PC. The Contributions of the Ribosome Biogenesis Protein Utp5/WDR43 to Craniofacial Development. Journal Of Dental Research 2016, 95: 1214-1220. PMID: 27221611, PMCID: PMC5076753, DOI: 10.1177/0022034516651077.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsRibosomal proteinsCraniofacial developmentRibosome biogenesis proteinsRibosomal protein functionHuman craniofacial developmentTissue-specific defectsDiverse cell typesHuman ribosomopathiesBiogenesis proteinsTranslational machineryProtein functionDistinct functionsTranslational mechanismsTissue differentiationCell typesWDR43Global defectsProteinExciting researchSurprising similaritiesUnderstanding of rolesRibosomopathiesBiogenesisMachineryMutations
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