Development and Characterization of an Inducible Bacterial Artificial Chromosome System for Studying Lytic Replication and Pathogenesis of Kaposi's Sarcoma‐Associated Herpesvirus
Xu X, Dong P, Li W, Wang X, Ming Z, Liu Z, Zhu F, Liang Q. Development and Characterization of an Inducible Bacterial Artificial Chromosome System for Studying Lytic Replication and Pathogenesis of Kaposi's Sarcoma‐Associated Herpesvirus. Journal Of Medical Virology 2025, 97: e70392. PMID: 40358030, DOI: 10.1002/jmv.70392.Peer-Reviewed Original ResearchConceptsBacterial artificial chromosomeKSHV lytic replicationLytic replicationViral proteinsCellular genesBAC backboneBacterial artificial chromosome systemContext of virus infectionTargets of viral proteinsFused fluorescent proteinsHygromycin selectable markerPathogenesis of Kaposi's sarcoma-associated herpesvirusKaposi's sarcoma-associated herpesvirusEfficient lytic replicationChromosome systemArtificial chromosomeHerpesvirus genomesSarcoma-associated herpesvirusSubcellular localizationSelectable markerResponse elementGene knockoutFluorescent proteinCRISPR-Cas9Progeny virus
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