The principles upon which MRM rest are based on a very robust MS technology that has been used for many years to quantify a wide range of small molecules in clinical samples. This high-throughput MS method has a wide linear dynamic range of up to five orders of magnitude and also has very high sensitivity that allows detection of ng/ml concentrations of pre-selected peptides from tryptic digests of human plasma and other complex samples such as cell/tissue protein extracts.
Rinehart, J. , Maksimova, Y.D. , Tanis, J.E. , Stone, K.L., Hodson, C.A., Zhang, J. , Risinger, M., Pan, W., Wu, D., Colangelo, C.M., Forbush, B., Joiner, C.H., Gulcicek, E.E., Gallagher, P.G., and Lifton, R.P. (2009) Sites of Regulated Phosphorylation that Control K-Cl Cotransporter Activity. Cell. 138(3):525-536.