Mixed Base Oligos: Equimolar (degenerate) and Non-Equimolar (doped)
Equimolar (Degenerate) Oligos:
Equimolar (degenerate) oligos can be ordered via the "Order Normal DNA..." link as long as the single-letter codes for the mixtures are used:
The codes are:
|M = (AC)||V = (AGC)||N = (AGCT)|
|R = (AG)||H = (ACT)|
|W = (AT)||D = (AGT)|
|S = (GC)||B = (GCT)|
|Y = (CT)|
|K = (GT)|
Note: AN OLIGO WITH A MIXED BASE AT THE 3' END CAN ONLY BE SYNTHESIZED AT THE 0.2 OR 1.0 SCALE. IF YOU PLACE AN ORDER FOR SUCH AN OLIGO AT THE 40 NANOMOLE SCALE, WE WILL CHANGE THE SCALE TO 0.2 MICROMOLES.
Non-equimolar (Doped) Oligos:
- Oligos containing non-equimolar mixtures must be ordered via Order Modified Oligos.
- Indicate the percentages of each base in the order of A : G : C : T. Assign a code to each mixture: 5, 6, 7, 8, 9, etc. and use that code in the sequence. Do NOT use upper- or lower-case A, G, C, or T to code for mixtures.
- Non-equimolar mixtures are not possible at the 3’ end.
- There is a surcharge for every 3ml bottle of a doped mixture. In order to calculate the volume needed for your order, please see our reagent usage table