Vijay Menon
Associate Research ScientistCards
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Research
Publications
2026
Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes
Heer C, Elia J, Menon V, Johnson S, Arbelaez S, Friedman S, Lopez-Giraldez F, Sundaram R, Herzon S, Bindra R, Gueble S. Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes. NAR Cancer 2026, 8: zcaf052. PMID: 41522810, PMCID: PMC12783042, DOI: 10.1093/narcan/zcaf052.Peer-Reviewed Original ResearchConceptsDNA-damaging agentsDNA repair genesTranscription-coupled nucleotide excision repairCRISPR knockout screenFanconi anemia pathwayRepair genesClasses of DNA-damaging agentsDNA damage repairChemical diversityNucleotide excision repairKnockout screenMethylation lesionsRepair of lesionsDamaging agentsDDR pathwaysDSB repairGenetic instabilityLN229 glioma cellsExcision repairDamage repairDNA lesionsMonofunctional alkylating agentsTopoisomerase IDNAGenes
2025
Reversing lysosomal dysfunction restores youthful state in aged hematopoietic stem cells
Arif T, Qiu J, Khademian H, Lohithakshan A, Menon A, Menon V, Slavinsky M, Batignes M, Lin M, Sebra R, Beaumont K, Benson D, Tzavaras N, Ménager M, Ghaffari S. Reversing lysosomal dysfunction restores youthful state in aged hematopoietic stem cells. Cell Stem Cell 2025, 32: 1904-1922.e7. PMID: 41289991, DOI: 10.1016/j.stem.2025.10.012.Peer-Reviewed Original ResearchConceptsHematopoietic stem cellsAging hematopoietic stem cellsCyclic GMP-AMP synthase-stimulatorHematopoietic stem cell agingStem cellsIn vivo repopulating capacityYouthful stateLysosomal processingMyeloid malignanciesRepopulating capacityClonal hematopoiesisLysosomal dysfunctionHematopoietic functionImmune declineEpigenetic homeostasisSelf-RenewalSingle-cell transcriptomicsInterferon genesDysfunctionLysosomal integrityLysosomal inhibitionVacuolar ATPaseAgeCellsMalignancyDNA repair helicases: from mechanistic understanding to therapeutic implications
Menon V, Gueble S. DNA repair helicases: from mechanistic understanding to therapeutic implications. NAR Cancer 2025, 7: zcaf034. PMID: 41064806, PMCID: PMC12501782, DOI: 10.1093/narcan/zcaf034.Peer-Reviewed Original ResearchConceptsHelicase functionDNA helicaseMaintenance of genome integrityHuman DNA helicaseNormal cell physiologyResolution of DNA damageSynthetic lethal interactionsATP-dependent enzymesDNA damage responseDNA repair processesProtein playersGenome integrityHelicase mutationsDNA double helixRothmund-Thomson syndromeCell physiologyLethal interactionsHelicaseGenomic instabilityDamage responseCellular DNABloom syndromeHuman disordersDNAFanconi anemia
2024
Mitochondrial regulation of erythropoiesis in homeostasis and disease
Menon V, Slavinsky M, Hermine O, Ghaffari S. Mitochondrial regulation of erythropoiesis in homeostasis and disease. British Journal Of Haematology 2024, 205: 429-439. PMID: 38946206, PMCID: PMC11619715, DOI: 10.1111/bjh.19600.Peer-Reviewed Original ResearchClearance of mitochondriaComplex maturation processCell maturation processMitochondrial DNAMitochondrial participationChromatin condensationMitochondrial regulationMaturation processRetention of mitochondriaErythroid cell maturationNuclear expulsionMitochondriaErythroblast enucleationRed blood cell productionMature red blood cellsErythroid maturationRegulation of erythropoiesisCell maturationErythroid cellsHomeostasisCellsDisease conditionsRed blood cellsChromatinOrganelles
2023
Antitumor active trans‑platinum complexes through metabolic stability and enhanced cellular accumulation
Menon V, Katner S, Lee D, Peterson E, Koblinski J, Farrell N. Antitumor active trans‑platinum complexes through metabolic stability and enhanced cellular accumulation. Journal Of Inorganic Biochemistry 2023, 252: 112475. PMID: 38199050, DOI: 10.1016/j.jinorgbio.2023.112475.Peer-Reviewed Original ResearchElevated CDKN1a (p21) mediates β-thalassemia erythroid apoptosis but its loss does not improve β-thalassemic erythropoiesis
Liang R, Lin M, Menon V, Qiu J, Menon A, Breda L, Arif T, Rivella S, Ghaffari S. Elevated CDKN1a (p21) mediates β-thalassemia erythroid apoptosis but its loss does not improve β-thalassemic erythropoiesis. Blood Advances 2023, 7: 6873-6885. PMID: 37672319, PMCID: PMC10685172, DOI: 10.1182/bloodadvances.2022007655.Peer-Reviewed Original ResearchConceptsErythroid cell maturationErythroid cell survivalCell cycle inhibitor Cdkn1aFoxO3 target genesErythroid apoptosisFoxO3 transcription factorElevated reactive oxygen speciesΒ-globin geneCell maturationEmbryonic lethalityTranscription factorsTarget genesErythroid maturationReactive oxygen speciesMolecular networksMolecular mechanismsThalassemic erythropoiesisCell survivalProgenitor compartmentErythroid compartmentFOXO3Extramedullary erythropoiesisΒ-thalassemiaApoptosisHemoglobin productionNext-generation sequencing methodologies to detect low-frequency mutations: “Catch me if you can”
Menon V, Brash D. Next-generation sequencing methodologies to detect low-frequency mutations: “Catch me if you can”. Mutation Research/Reviews In Mutation Research 2023, 792: 108471. PMID: 37716438, PMCID: PMC10843083, DOI: 10.1016/j.mrrev.2023.108471.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2022
Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging
Qiu J, Menon V, Tzavaras N, Liang R, Ghaffari S. Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging. STAR Protocols 2022, 3: 101828. PMID: 36595934, PMCID: PMC9676629, DOI: 10.1016/j.xpro.2022.101828.Peer-Reviewed Original Research
2021
Mitochondrial localization and moderated activity are key to murine erythroid enucleation
Liang R, Menon V, Qiu J, Arif T, Renuse S, Lin M, Nowak R, Hartmann B, Tzavaras N, Benson DL, Chipuk JE, Fribourg M, Pandey A, Fowler V, Ghaffari S. Mitochondrial localization and moderated activity are key to murine erythroid enucleation. Blood Advances 2021, 5: 2490-2504. PMID: 34032849, PMCID: PMC8152511, DOI: 10.1182/bloodadvances.2021004259.Peer-Reviewed Original ResearchConceptsHigh-throughput single-cell imagingExtensive cellular remodelingActive mitochondrial respirationMammalian red blood cellsSingle-cell imagingMitochondrial membrane potentialErythroid enucleationHigh mitochondrial membrane potentialLoss of mitochondriaUnanticipated functionMitochondrial localizationMitochondrial metabolismTerminal stepErythroid lineageErythroblast maturationCellular remodelingNuclear expulsionMitochondrial respirationDistinct functional subpopulationsMitochondriaMitochondrial activityCellular contentNuclear clearanceConfocal microscopyEnucleation processErythroid enucleation: a gateway into a “bloody” world
Menon V, Ghaffari S. Erythroid enucleation: a gateway into a “bloody” world. Experimental Hematology 2021, 95: 13-22. PMID: 33440185, PMCID: PMC8147720, DOI: 10.1016/j.exphem.2021.01.001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBirdsCalciumCell NucleusChromatinColony-Forming Units AssayComputational BiologyCytokinesCytoskeletal ProteinsDNA-Binding ProteinsErythroblastsErythrocytesErythropoiesisIntercellular Signaling Peptides and ProteinsMammalsMiceMicroRNAsProto-Oncogene ProteinsReceptors, Thyroid HormoneRepressor ProteinsReticulocytesrho GTP-Binding ProteinsTranscription FactorsTransport VesiclesYolk SacConceptsTerminal erythroid maturationFormation of reticulocytesEnucleation processHematopoietic stem cellsTranscription factorsCytoskeletal proteinsErythroid maturationFascinating processOrthochromatic erythroblastsTerminal maturationRate-limiting stepStem cellsIntricate processMaturationRed blood cellsCellsRBC productionMicroRNAsProteinProduction ex vivoErythroblastsEx vivoMechanismDaily productionProduction
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