Voluntary Faculty
Voluntary faculty are typically clinicians or others who are employed outside of the School but make significant contributions to department programs at the medical center or at affiliate institutions.
Voluntary rank detailsEdward Ryan, PhD
Associate Clinical ProfessorAbout
Research
Publications
2025
Gut bacteria-derived sphingolipids alter innate immune responses to oral cholera vaccine antigens
Chac D, Heller F, Banna H, Kaisar M, Markiewicz S, Pruitt E, Chowdhury F, Bhuiyan T, Akter A, Khan A, Dumayas M, Rice A, Karmakar P, Dash P, LaRocque R, Ryan E, Xu L, Minot S, Harris J, Qadri F, Weil A. Gut bacteria-derived sphingolipids alter innate immune responses to oral cholera vaccine antigens. Nature Communications 2025 PMID: 41388019, DOI: 10.1038/s41467-025-67388-y.Peer-Reviewed Original ResearchOral cholera vaccineMetagenomic sequencing dataStrain-specific resultsSequence dataMetagenomic sequencingVibrio choleraeFecal microbiotaImmune responseOral vaccine responsesBiological outcomesMicrobiotaHuman macrophagesSphingolipidsVaccine responseVaccine antigensCholera vaccineAntigenBacteroidesBacteriaGutSequenceVaccineDegree of protectionCholeraClinical and environmental wastewater-based bacteriophage surveillance for high-impact diarrheal diseases, including cholera, in Bangladesh.
Akhtar M, Amin M, Hussain S, Nafsi N, Parvin N, Khanam F, Islam M, Islam Bhuiyan M, Afroz R, Firoj M, Chowdhury F, Khan A, Jubair M, Ryan E, Shapiro B, Thomson N, Nelson E, Rahman M, Begum Y, Bhuiyan T, Qadri F. Clinical and environmental wastewater-based bacteriophage surveillance for high-impact diarrheal diseases, including cholera, in Bangladesh. MBio 2025, e0265425. PMID: 41363802, DOI: 10.1128/mbio.02654-25.Peer-Reviewed Original ResearchDiarrheal pathogensBacterial hostsPotential of bacteriophagesEscherichia coliBacterial counterpartsPhage dynamicsPathogen populationsETECDiarrheal patientsPhageDiarrheal specimensHost rangeDiarrheal surveillancePathogensEnvironmental sourcesEarly indicatorBacteriophageDiarrheal diseasePathogen transmissionLt;5 yearsEndemic settingsDiarrhealCholeraHostTransmission dynamicsTravel After Transplant: Demographics, Travel Trends, and Vaccination Practices of US Immunocompromised Travelers.
Rolfe R, Rao S, Oliver E, Thwing J, Ryan E, Kotton C, LaRocque R. Travel After Transplant: Demographics, Travel Trends, and Vaccination Practices of US Immunocompromised Travelers. Transplantation 2025 PMID: 41288311, DOI: 10.1097/tp.0000000000005580.Peer-Reviewed Original ResearchTravel trendsPretravel health consultationCruise ship travelControl travelOnline structured questionnaireVaccination practicesTravel patternsTravel itinerariesVisiting friendsTravelHigher-risk populationsPretravel preparationShip travelHealth preparationStem cell transplant recipientsRisk of illnessHealth consultationUS clinical sitesDescriptive statisticsStructured questionnaireClinical sitesMeasles-mumps-rubella vaccineYellow fever vaccineMedian ageTransplant recipientsTravel Healthy, a mobile app for participatory surveillance among U.S. international travelers
Colubri A, Willing N, Grozdani A, Dong Y, Hong H, Khandpekar M, Oliver E, Thwing J, Ryan E, LaRocque R. Travel Healthy, a mobile app for participatory surveillance among U.S. international travelers. Travel Medicine And Infectious Disease 2025, 68: 102922. PMID: 41083032, DOI: 10.1016/j.tmaid.2025.102922.Peer-Reviewed Original ResearchExpanding cholera serosurveillance to vaccinated populations
Jones F, Bhuiyan T, Slater D, Ternier R, Vater K, Khan A, Chowdhury F, Visieres K, Biswas R, Kamruzzaman M, Ryan E, Calderwood S, LaRocque R, Charles R, Leung D, Lessler J, Ivers L, Qadri F, Harris J, Azman A. Expanding cholera serosurveillance to vaccinated populations. MBio 2025, 16: e01898-25. PMID: 41055373, PMCID: PMC12607766, DOI: 10.1128/mbio.01898-25.Peer-Reviewed Original ResearchConceptsSerological surveillanceToxin-coregulated pilus ACholera toxin B subunitSeroincidence estimatesB subunitToxin B subunitMonths post-vaccinationPost-vaccinationEpidemiological toolAntibody dynamicsCholeraIgA bindingEpidemiological monitoringRandom forest modelClinical surveillanceComplementary approachesAssociated with infectionImmune responseVaccination statusAntibody responseMultiplex bead assayForest modelLongitudinal serum samplesSerum samplesAntibodiesInsights Into Global Antimicrobial Resistance Dynamics Through the Sequencing of Enteric Bacteria From US International Travelers
Sridhar S, Worby C, Bronson R, Turbett S, Oliver E, Shea T, Rao S, Sanchez V, Becker M, Holliday L, Slater D, Harris J, Walters M, Walker A, Knouse M, Leung D, Kelly P, Ryan E, LaRocque R, Earl A. Insights Into Global Antimicrobial Resistance Dynamics Through the Sequencing of Enteric Bacteria From US International Travelers. Journal Of Infectious Diseases 2025, jiaf469. PMID: 40990287, DOI: 10.1093/infdis/jiaf469.Peer-Reviewed Original ResearchBlaCTX-M-55Antimicrobial resistance dynamicsGenomic neighborhoodAntimicrobial resistanceDistribution of AMR genesPresence of extended-spectrum beta-lactamasesAvian pathogenic E. coliPrevalence of resistance genesExtended-spectrum beta-lactamasePathogenic E. coliAssociated with geographyColistin-resistant EnterobacteralesMcr-1 geneBlaCTX-M-27Phylogenetic diversityPlasmid reconstructionColV plasmidsCarbapenem-resistant EnterobacteralesAMR genesEscherichia coliGlobal distributionResistance genesAntimicrobial resistant organismsEnteric bacteriaBeta-lactamaseHuman antibody targeting Vibrio cholerae O1 O-specific polysaccharide induces an amotile hypovirulent bacterial phenotype: mechanism of protection against cholera
Verma S, Cetinbas M, Kelly M, Senger S, Faherty C, Janardhanan J, Wagh C, Bhuiyan T, Chowdhury F, Khan A, Akter A, Charles R, Harris J, Calderwood S, Wrammert J, Waldor M, Asp M, Tai J, Yan J, Xu P, Kováč P, Sadreyev R, Qadri F, Ryan E. Human antibody targeting Vibrio cholerae O1 O-specific polysaccharide induces an amotile hypovirulent bacterial phenotype: mechanism of protection against cholera. MBio 2025, 16: e02235-25. PMID: 40937849, PMCID: PMC12505965, DOI: 10.1128/mbio.02235-25.Peer-Reviewed Original ResearchAnti-OSP antibodiesO-specific polysaccharideVibrio cholerae,Bacterial motilityLevels of cyclic di-GMPInhibition of bacterial motilityEnteroid modelCyclic di-GMPCholera toxin detectionIntestinal surfaceCulture-based assaysInhibition of motilityVirulence programInfect humansRegulon expressionDi-GMPVirulence genesVirulence mechanismsBacterial phenotypesPathogen physiologyPresence of mucinExtracellular matrix componentsBiofilm formationBacterial metabolismIntestinal mucusGenomic insights into a diarrheal outbreak in Bangladesh reveal novel ETEC lineages and expansion of CS23 colonization factor
Rahman S, Jubair M, Akhtar M, Akter A, Tauheed I, Begum Y, Bhattacharjee P, Afrad M, Khanam F, Islam M, Khan A, Rahman M, Ryan E, Fleckenstein J, Bhuiyan T, Thomson N, Qadri F, von Mentzer A, Chowdhury F. Genomic insights into a diarrheal outbreak in Bangladesh reveal novel ETEC lineages and expansion of CS23 colonization factor. Microbiology Spectrum 2025, 13: e03315-24. PMID: 40928300, PMCID: PMC12502627, DOI: 10.1128/spectrum.03315-24.Peer-Reviewed Original ResearchColonization factorsETEC strainsNovel lineagesGenomic surveillanceDiarrheal outbreaksETEC-associated diarrheaWhole-genome sequencingEscherichia coliETEC isolatesETEC infectionGenomic attributesGenomic diversityETEC toxinsGenome sequenceGenomic insightsPhylogenetic analysisVirulence profilesPopulation structureVirulence factorsAMR genesCause of diarrheaETECEvolutionary changesDiarrheal casesResistance mechanismsEvaluation of Cholera Antigen-Specific Gut-Homing β7-Positive Antibody-Secreting Cells in the Systemic Circulation of Oral Cholera Vaccinees Receiving Doses at Different Intervals
Karmakar P, Rashu R, Hoq M, Salma U, Islam K, Jahan N, Nishat N, Akter A, Jahan R, Dash P, Saha A, Ryan E, Qadri F, Bhuiyan T. Evaluation of Cholera Antigen-Specific Gut-Homing β7-Positive Antibody-Secreting Cells in the Systemic Circulation of Oral Cholera Vaccinees Receiving Doses at Different Intervals. Vaccines 2025, 13: 919. PMID: 41012122, PMCID: PMC12474143, DOI: 10.3390/vaccines13090919.Peer-Reviewed Original ResearchTwo-dose regimenAntibody-secreting cellsOral cholera vaccineT cell responsesSingle doseSystemic circulationDoses of oral cholera vaccineSingle dose of oral cholera vaccineSingle-dose strategyTwo-dose groupSingle dose of vaccineSingle-dose vaccinationDose of vaccineYears of ageCytotoxic memoryDay 7 post-vaccinationGut-homingGut homingCholera-endemic regionsImmune responseShancholShort-term protectionAdult volunteersGlobal health challengeRegimenVibrio cholerae-specific antibodies in plasma and saliva in cholera patients during a severe outbreak in Zambia: an antibody profiling approach
Luchen C, Ngo’mbe H, Liswaniso F, Bosomprah S, Zhiteneva J, Harris J, Charles R, Ryan E, Sack D, Bernshtein B, Chisenga C. Vibrio cholerae-specific antibodies in plasma and saliva in cholera patients during a severe outbreak in Zambia: an antibody profiling approach. Frontiers In Immunology 2025, 16: 1641319. PMID: 40895561, PMCID: PMC12394519, DOI: 10.3389/fimmu.2025.1641319.Peer-Reviewed Original ResearchConceptsOgawa O-specific polysaccharideElevated biomarker levelsResource-limited settingsNon-invasive alternativeAntibody profilesLevels of total IgGVaccine immunogenicity studiesTreatment centresMucosal immune responsesTotal IgG levelsPlasma-based assaysCholera toxin BIgG1-3Resource-constrained settingsIgG levelsMucosal pathogensPublic health threatMucosal immunityAntibody-based profilingImmunogenicity studiesImmune responseTotal IgGSecretory IgASalivary responseImmunological differences
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