2008
The role of G proteins in thromospondin-1–induced vascular smooth muscle cell migration
Fuse S, Esemuede N, Yamaguchi M, Maier KG, Nesselroth SM, Sumpio BE, Gahtan V. The role of G proteins in thromospondin-1–induced vascular smooth muscle cell migration. Surgery 2008, 144: 86-92. PMID: 18571589, DOI: 10.1016/j.surg.2008.03.028.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsTSP-1-induced chemotaxisExtracellular signal-regulated kinase 1/2Pertussis toxinSerum-free mediumCholera toxinThrombospondin-1CAMP levelsP38 activationG proteinsVascular smooth muscle cell migrationQuiescent vascular smooth muscle cellsSmooth muscle cell migrationCyclic adenosine monophosphate levelsSmooth muscle cellsIntracellular cyclic adenosine monophosphate (cAMP) levelsMuscle cell migrationTSP-1 receptorAdenosine monophosphate levelsSignal-regulated kinase 1/2Levels of cAMPArterial lesionsVascular injuryVSMC chemotaxisMicrochemotaxis chamber
2005
Homocysteine promotes p38-dependent chemotaxis in bovine aortic smooth muscle cells
Akasaka K, Akasaka N, Di Luozzo G, Sasajima T, Sumpio BE. Homocysteine promotes p38-dependent chemotaxis in bovine aortic smooth muscle cells. Journal Of Vascular Surgery 2005, 41: 517-522. PMID: 15838488, DOI: 10.1016/j.jvs.2004.12.043.Peer-Reviewed Original ResearchConceptsEffect of homocysteineMigration of SMCsLevels of homocysteineProgressive intimal thickeningAortic smooth muscle cellsSmooth muscle cell migrationBovine aortic smooth muscle cellsPotential therapeutic implicationsSmooth muscle cellsP38 activationExposure of SMCMuscle cell migrationSMC chemotaxisRisk factorsSelective blockadeIntimal thickeningTherapeutic implicationsP38-dependent pathwaySMC proliferationBoyden chamberChemotactic potentialMuscle cellsHomocysteineFetal bovine serumP38 inhibitor
2003
Thrombospondin‐1‐induced vascular smooth muscle cell chemotaxis: The role of the type 3 repeat and carboxyl terminal domains
Lee T, Nesselroth SM, Olson ET, Esemuede N, Lawler J, Sumpio BE, Gahtan V. Thrombospondin‐1‐induced vascular smooth muscle cell chemotaxis: The role of the type 3 repeat and carboxyl terminal domains. Journal Of Cellular Biochemistry 2003, 89: 500-506. PMID: 12761883, DOI: 10.1002/jcb.10524.Peer-Reviewed Original ResearchConceptsVSMC chemotaxisThrombospondin-1Acute phase reactantsSmooth muscle cell chemotaxisVascular diseaseVascular smooth muscle cell chemotaxisPhase reactantsExtracellular signal-regulated kinases 1Microchemotaxis chamberSignal-regulated kinases 1Serum-free mediumCell chemotaxisMatricellular glycoproteinActivate ERKWestern immunoblottingVSMCsT-testP38 activationP38 pathwayERKFusion proteinT3ChemotaxisKinase 1P38 kinase
2002
SPHINGOSINE-1-PHOSPHATE STIMULATES HUMAN CACO-2 INTESTINAL EPITHELIAL PROLIFERATION VIA p38 ACTIVATION AND ACTIVATES ERK BY AN INDEPENDENT MECHANISM
THAMILSELVAN V, LI W, SUMPIO BE, BASSON MD. SPHINGOSINE-1-PHOSPHATE STIMULATES HUMAN CACO-2 INTESTINAL EPITHELIAL PROLIFERATION VIA p38 ACTIVATION AND ACTIVATES ERK BY AN INDEPENDENT MECHANISM. In Vitro Cellular & Developmental Biology - Animal 2002, 38: 246-253. PMID: 12197778, DOI: 10.1290/1071-2690(2002)038<0246:spshci>2.0.co;2.Peer-Reviewed Original ResearchConceptsExtracellular signal-regulated kinases 1Mitogen-activated protein kinaseMAP kinase kinaseCaco-2 proliferationMAPK activationHuman intestinal epithelial proliferationP38 activationCell typesSignal-regulated kinases 1Role of ERKMitogenic effectCaco-2 intestinal epithelial cellsIntracellular second messengerMEK inhibitionP38 MAPK activationCancer cell invasionKinase kinaseHuman Caco-2 intestinal epithelial cellsProtein kinaseStimulation of proliferationCell motilityIntestinal epithelial cell proliferationInhibitor PD98059ERK2ERK activation