1999
Extracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain
Ikeda M, Takei T, Mills I, Kito H, Sumpio B. Extracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain. American Journal Of Physiology 1999, 276: h614-h622. PMID: 9950863, DOI: 10.1152/ajpheart.1999.276.2.h614.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCalciumCalcium-Calmodulin-Dependent Protein KinasesCattleCell DivisionCells, CulturedEndothelium, VascularEnzyme ActivationEnzyme InhibitorsExtracellular SpaceFlavonoidsIntracellular MembranesPhosphorylationProtein Kinase CProtein-Tyrosine KinasesStress, MechanicalConceptsERK1/ERK2Bovine aortic endothelial cellsExtracellular signal-regulated kinases 1ERK1/ERK2 activationExtracellular signal-regulated kinases 1/2Signal-regulated kinases 1/2Tyrosine kinase inhibitor genisteinCell proliferationTreatment of BAECProtein kinase C activationProtein kinase C inhibitor calphostin CTime-dependent phosphorylationKinase inhibitor genisteinKinase C activationERK2 activationEndothelial cellsERK2Strain-induced activationKinase 1Kinase 1/2PD 98059Tyrosine kinaseInhibitor genisteinCalphostin CStrain-dependent activation
1992
Immunocytochemical expression and localization of protein kinase C in bovine aortic endothelial cells
Rosales O, Isales C, Nathanson M, Sumpio B. Immunocytochemical expression and localization of protein kinase C in bovine aortic endothelial cells. Biochemical And Biophysical Research Communications 1992, 189: 40-46. PMID: 1449492, DOI: 10.1016/0006-291x(92)91522-r.Peer-Reviewed Original ResearchConceptsSerum-deprived mediumPKC activityPhorbol ester-induced increaseAortic endothelial cellsBovine aortic endothelial cellsBeta stainingSDM groupTotal PKC activityImmunocytochemical expressionBeta PKC isoformsEndothelial cellsParticulate fractionFBS groupProtein kinase CCalphostin CPKC inhibitorPhorbol esterConfocal microscopyDiffuse cytoplasmic localizationCytosolic fractionGamma-PKCKinase CPKC isoformsCytoplasmic localizationCellsProtein kinase C is a mediator of the adaptation of vascular endothelial cells to cyclic strain in vitro.
Rosales OR, Sumpio BE. Protein kinase C is a mediator of the adaptation of vascular endothelial cells to cyclic strain in vitro. Surgery 1992, 112: 459-66. PMID: 1379381.Peer-Reviewed Original ResearchConceptsProtein kinase CActivation of PKCImmunocytochemical stainingPKC activityCyclic stretchEndothelial cell activationVascular endothelial cellsCycles/minSustained elevationCell activationFlexible-bottomed culture platesKinase CBovine aortic ECsECS resultsEndothelial cellsBlood vesselsAortic ECsEvidence of translocationSpecific PKC inhibitorEC proliferationCalphostin CEC growthHemodynamic forces