2022
Placental macrophage responses to viral and bacterial ligands and the influence of fetal sex
Pantazi P, Kaforou M, Tang Z, Abrahams V, McArdle A, Guller S, Holder B. Placental macrophage responses to viral and bacterial ligands and the influence of fetal sex. IScience 2022, 25: 105653. PMID: 36505933, PMCID: PMC9732417, DOI: 10.1016/j.isci.2022.105653.Peer-Reviewed Original ResearchPathogen-associated molecular patternsViral pathogen-associated molecular patternsHofbauer cellsFetal sexMajor histocompatibility complex class IAdverse pregnancy outcomesHistocompatibility complex class IToll-like receptorsComplex class ISex-dependent responseInfectious triggerPregnancy outcomesPlacental macrophagesVertical pathogen transmissionViral infectionLipid metabolismMacrophage responseBacterial ligandsProtein expression changesClass IMolecular patternsPlacentaMacrophagesCytoskeleton organizationQuantitative proteomics
2018
Role of NOD2 in antiphospholipid antibody-induced and bacterial MDP amplification of trophoblast inflammation
Mulla MJ, Pasternak MC, Salmon JE, Chamley LW, Abrahams VM. Role of NOD2 in antiphospholipid antibody-induced and bacterial MDP amplification of trophoblast inflammation. Journal Of Autoimmunity 2018, 98: 103-112. PMID: 30594350, DOI: 10.1016/j.jaut.2018.12.003.Peer-Reviewed Original ResearchConceptsMuramyl dipeptideAbsence of aPLAnti-angiogenic profileNovel danger signalPresence of aPLRole of NOD2IL-1β expressionMaternal-fetal interfaceActivation of NOD2IL-1β productionBacterial muramyl dipeptideAntiphospholipid antibodiesCommon bacterial componentsPregnancy complicationsPregnancy outcomesAdverse outcomesIL-1βTrophoblast responsesExtravillous trophoblastsHigh riskPreeclampsiaDanger signalsBacterial infectionsVEGF secretionInflammation
2017
Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation.
Cross SN, Potter JA, Aldo P, Kwon JY, Pitruzzello M, Tong M, Guller S, Rothlin CV, Mor G, Abrahams VM. Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation. The Journal Of Immunology 2017, 199: 2885-2895. PMID: 28916522, PMCID: PMC5659726, DOI: 10.4049/jimmunol.1700870.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedChorioamnionitisC-Mer Tyrosine KinaseExtraembryonic MembranesFemaleGammaherpesvirinaeHerpesviridae InfectionsHerpesvirus 2, HumanHumansImmunizationInflammasomesIntercellular Signaling Peptides and ProteinsInterleukin-1betaLipopolysaccharidesMiceMice, Inbred C57BLMice, KnockoutPregnancyPremature BirthProto-Oncogene ProteinsReceptor Protein-Tyrosine KinasesConceptsFetal membranesViral infectionImmune responseTAM receptorsSubsequent preterm birthAdverse pregnancy outcomesIL-1β productionIL-1β responseHerpes viral infectionsInnate immune responseVivo mouse modelHuman fetal membranesAggravated inflammationIL-1β processingPregnancy outcomesPreterm birthAssociated complicationsPremature rupturePregnant womenLocal infectionInflammasome activationMerTK inhibitionMouse modelBacterial LPSLigand expression
2014
Single- and double-stranded viral RNA generate distinct cytokine and antiviral responses in human fetal membranes
Bakaysa SL, Potter JA, Hoang M, Han CS, Guller S, Norwitz ER, Abrahams VM. Single- and double-stranded viral RNA generate distinct cytokine and antiviral responses in human fetal membranes. Molecular Human Reproduction 2014, 20: 701-708. PMID: 24723465, PMCID: PMC4072183, DOI: 10.1093/molehr/gau028.Peer-Reviewed Original ResearchConceptsHuman fetal membranesToll-like receptorsMIP-1βMIP-1αFetal membranesAntiviral responseIL-1βIL-6IL-2Viral infectionG-CSFCytokine/chemokine profilesViral ssRNAAdverse pregnancy outcomesMaternal-fetal interfaceInfection-associated inflammationMCP-1 secretionViral dsRNA analogueTNF-α productionMIP-1α secretionTLR adapter proteinsExpression of IFNβPregnancy outcomesCytokine profileChemokine profiles
2011
Nod1 Activation by Bacterial iE-DAP Induces Maternal–Fetal Inflammation and Preterm Labor
Cardenas I, Mulla MJ, Myrtolli K, Sfakianaki AK, Norwitz ER, Tadesse S, Guller S, Abrahams VM. Nod1 Activation by Bacterial iE-DAP Induces Maternal–Fetal Inflammation and Preterm Labor. The Journal Of Immunology 2011, 187: 980-986. PMID: 21677137, DOI: 10.4049/jimmunol.1100578.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCell LineDiaminopimelic AcidDisease Models, AnimalFemaleHumansInfant, NewbornInfant, PrematureInflammationMaternal-Fetal ExchangeMiceMice, Inbred C57BLNod1 Signaling Adaptor ProteinObstetric Labor, PrematurePregnancyPregnancy OutcomeTissue Culture TechniquesTrophoblastsConceptsPattern recognition receptorsPreterm laborCytokine profileNOD1 activationRecognition receptorsInfection-associated preterm laborHuman first-trimester trophoblastsIntracellular pattern recognition receptorsProinflammatory cytokine profileHuman term placental tissueActivation of NOD1Maternal-fetal interfaceInfection-associated inflammationFunction of NOD1Term placental tissueFirst trimester trophoblastsThird trimester trophoblastFetal inflammationPreterm deliveryEmbryonic day 14.5Pregnancy outcomesFetal weightIL-6MCP-1NOD2 stimulation