2017
Regulation of glomerulotubular balance: flow-activated proximal tubule function
Wang T, Weinbaum S, Weinstein A. Regulation of glomerulotubular balance: flow-activated proximal tubule function. Pflügers Archiv - European Journal Of Physiology 2017, 469: 643-654. PMID: 28271233, PMCID: PMC6162058, DOI: 10.1007/s00424-017-1960-8.Peer-Reviewed Original ResearchConceptsNa/H exchanger isoform 3Proximal tubulesTubule functionIntracellular Ca2+ signalingProximal tubule transportMammalian proximal tubuleAcid-base transportersProximal tubule functionRegulation of sodiumCa2+ signalingHCO3- absorptionStimulate Na+Tubule transportMicropuncture studiesSolute reabsorptionPeritubular membraneBrush border membraneBrush border microvilliGlomerulotubular balanceHCO3- transportIsoform 3Flow velocity changesTubulesRegulatory mechanismsTransporter availability
2016
Flow-activated proximal tubule function underlies glomerulotubular balance.
Du Z, Duan Y, Yan Q, Weinbaum S, Weinstein A, Wang T. Flow-activated proximal tubule function underlies glomerulotubular balance. 2016, 46: 105-117. PMID: 31105444, PMCID: PMC6519745.Peer-Reviewed Original ResearchProximal tubulesModulating Na<sup>+</sup>Isolated perfused proximal tubulesTubule cell volumeExchanger isoform 3Proximal tubule cellsProximal tubule functionPerfused proximal tubulesH-ATPase activityMouse tubulesNHE3 expressionStimulate sodiumApical membraneTubule functionBicarbonate absorptionTubule cellsReceptor-mediatedGlomerulotubular balanceMouse kidneyIsoform 3H-ATPaseCellular mechanismsTubulesTransporter densityNHE3
2013
REGULATION OF GLOMERULOTUBULAR BALANCE IV: IMPLICATION OF AQUAPORIN 1 IN FLOW‐DEPENDENT TRANSPORT AND CELL VOLUME
Du Z, Yan Q, Weinbaum S, Weinstein A, Wang T. REGULATION OF GLOMERULOTUBULAR BALANCE IV: IMPLICATION OF AQUAPORIN 1 IN FLOW‐DEPENDENT TRANSPORT AND CELL VOLUME. The FASEB Journal 2013, 27: 912.27-912.27. DOI: 10.1096/fasebj.27.1_supplement.912.27.Peer-Reviewed Original ResearchAQP-1Proximal tubulesKO miceWild-typeMicroperfused in vitroTransepithelial osmotic gradientHCO-3 transportWT miceNo significant differencePeritubular transportHCO-3 absorptionVolume reabsorptionPeritubular membraneWater reabsorptionAquaporin-1Mouse kidneyMicePerfusion rateH-ATPaseSignificant differenceTubulesReabsorptionOsmotic gradientCell volumePerfusion
2012
Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport
Du Z, Wan L, Yan Q, Weinbaum S, Weinstein A, Wang T. Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f1507-f1516. PMID: 22952281, PMCID: PMC3532483, DOI: 10.1152/ajprenal.00277.2012.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAngiotensin II Type 1 Receptor BlockersAnimalsBicarbonatesBiological TransportEnzyme InhibitorsFemaleHemostasisIn Vitro TechniquesKidney GlomerulusKidney TubulesLosartanMacrolidesMiceMice, KnockoutModels, AnimalProton-Translocating ATPasesReceptor, Angiotensin, Type 1SodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsNa(+)-H(+) exchanger 3Mouse tubulesImpact of angiotensin IIH-ATPaseMicroperfused in vitroAT(2) receptorsAT(1A) receptorFlow-mediated changesH-ATPase activityParacellular Cl(-Receptor availabilityAngiotensin IIAT(1ARegulate Na(+Increased Na(+Local angiotensinBasolateral transportJ(HCO3Mouse kidneyNet fluidInhibitor bafilomycinProtein levelsMiceReceptorsTubules
2010
Mechanotransduction in the renal tubule
Weinbaum S, Duan Y, Satlin L, Wang T, Weinstein A. Mechanotransduction in the renal tubule. American Journal Of Physiology. Renal Physiology 2010, 299: f1220-f1236. PMID: 20810611, PMCID: PMC3006307, DOI: 10.1152/ajprenal.00453.2010.Peer-Reviewed Original ResearchConceptsCortical collecting ductCa(2+) signalingProximal tubulesModulation of Na(+Stretch-activated ion channelsDistal nephronRenal epitheliumWater reabsorptionRenal tubulesGlomerulotubular balanceInner earEndothelial cellsIon channelsHair cellsIntracellular signalingBone cellsCa(2TubulesPrimary ciliaIntracellular cytoskeletonCellsShear stressHydrodynamic forcesApical structuresFluid shear stressROLE OF DOPAMINE IN MODULATING FLOW‐DEPENDENT SODIUM AND BICARBONATE TRANSPORT IN MOUSE PROXIMAL TUBULE
Du Z, Yan Q, Weinbaum S, Weinstein A, Wang T. ROLE OF DOPAMINE IN MODULATING FLOW‐DEPENDENT SODIUM AND BICARBONATE TRANSPORT IN MOUSE PROXIMAL TUBULE. The FASEB Journal 2010, 24: 1024.2-1024.2. DOI: 10.1096/fasebj.24.1_supplement.1024.2.Peer-Reviewed Original ResearchProximal tubule transportEffects of dopamineMouse proximal tubuleTubule transportProximal tubulesNHE3 endocytosisCAMP-dependent protein kinase inhibitor H89PKA-mediated mechanismMicroperfused in vitroProtein kinase inhibitor H89Kidney proximal tubulesReabsorption of fluidLuminal flow rateKinase inhibitor H89Sodium reabsorptionApical membraneHCO-3 absorptionInhibitor H89DopaminePerfusion rateTubulesSCH23390NHE3Net reabsorptionSulpiride
2007
Flow-dependent transport in a mathematical model of rat proximal tubule
Weinstein A, Weinbaum S, Duan Y, Du Z, Yan Q, Wang T. Flow-dependent transport in a mathematical model of rat proximal tubule. American Journal Of Physiology. Renal Physiology 2007, 292: f1164-f1181. PMID: 17213461, DOI: 10.1152/ajprenal.00392.2006.Peer-Reviewed Original ResearchConceptsNa(+) reabsorptionRat proximal tubulesProximal tubulesModel tubuleDiuretic effectMouse proximal tubuleLuminal perfusion rateLow flowsSolute transportNaCl reabsorptionPeritubular transportTranscellular NaCl reabsorptionCarbonic anhydrase inhibitionGlomerulotubular balanceTubulesReabsorptionTransporter densityLuminal flowModel calculationsRatsFlow-dependent transportTransporter insertionMathematical modelPerfusion rateRate of hydrationFlow‐dependent transport in a mathematical model of rat proximal tubule
Weinstein A, Weinbaum S, Duan Y, Du Z, Yan Q, Wang T. Flow‐dependent transport in a mathematical model of rat proximal tubule. The FASEB Journal 2007, 21: a827-a828. DOI: 10.1096/fasebj.21.6.a827-d.Peer-Reviewed Original ResearchRat proximal tubulesNa+ reabsorptionProximal tubulesDiuretic effectModel tubuleOsmotic diuretic effectLow flowsSolute transportMouse tubulesLuminal perfusion rateNaCl reabsorptionPeritubular transportTranscellular NaCl reabsorptionCarbonic anhydrase inhibitionGlomerulotubular balanceTubulesReabsorptionTransporter densityLuminal flowMathematical modelRate of hydrationFlow-dependent transportInlet flowTransportTorque calculation
2006
Flow-activated transport events along the nephron
Wang T. Flow-activated transport events along the nephron. Current Opinion In Nephrology & Hypertension 2006, 15: 530-536. PMID: 16914967, DOI: 10.1097/01.mnh.0000242180.46362.c4.Peer-Reviewed Original ResearchConceptsBrush-border microvilliProximal tubulesCultured proximal tubule cellsProximal tubules in vitroGlomerular filtration rateProximal tubule cellsBrush-borderH-ATPase activityTubules in vitroNHE3 activityHCO3- absorptionENOS translocationTubule cellsFiltration rateMg2+ transportReceptor localizationSignal transduction mechanismsH-ATPaseHormonal regulationCa2+NephronTubulesResponse to flowTransduction mechanismsIntact actin cytoskeleton
2005
Role of PDZK1 in membrane expression of renal brush border ion exchangers
Thomson RB, Wang T, Thomson BR, Tarrats L, Girardi A, Mentone S, Soleimani M, Kocher O, Aronson PS. Role of PDZK1 in membrane expression of renal brush border ion exchangers. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 13331-13336. PMID: 16141316, PMCID: PMC1201624, DOI: 10.1073/pnas.0506578102.Peer-Reviewed Original ResearchConceptsProximal tubulesExpression of NHE3Functional activityRole of PDZK1Protein PDZK1Reabsorption of NaBrush border expressionMammalian kidneyCFEXBrush border localizationMutant miceGST fusion proteinProtein expressionKidneyBrush border membrane proteinsExchanger NHE3Membrane expressionNHE3Brush borderBrush border membrane vesiclesPDZK1 interactionTubulesNormal expressionMembrane proteinsPDZK1
2004
Renal and intestinal transport defects in Slc26a6-null mice
Wang Z, Wang T, Petrovic S, Tuo B, Riederer B, Barone S, Lorenz JN, Seidler U, Aronson PS, Soleimani M. Renal and intestinal transport defects in Slc26a6-null mice. American Journal Of Physiology - Cell Physiology 2004, 288: c957-c965. PMID: 15574486, DOI: 10.1152/ajpcell.00505.2004.Peer-Reviewed Original ResearchConceptsWild-type miceProximal tubulesSlc26a6-null miceHCO3- secretionKidney proximal tubulesApical membrane ClNull miceBaseline rateNormal blood pressureCl-/formate exchangeBlood pressureKidney functionElectrolyte profileMucosal tissuesIntestinal physiologyUssing chambersSmall intestineMiceFluid absorptionNaCl absorptionStatistical significanceCl-/HCO3NaCl transportDuodenumTubulesMechanosensory function of microvilli of the kidney proximal tubule
Du Z, Duan Y, Yan Q, Weinstein A, Weinbaum S, Wang T. Mechanosensory function of microvilli of the kidney proximal tubule. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 13068-13073. PMID: 15319475, PMCID: PMC516518, DOI: 10.1073/pnas.0405179101.Peer-Reviewed Original ResearchConceptsKidney proximal tubulesProximal tubulesNa+ reabsorptionNa+ transportNa+-H+ exchange activityProximal tubule Na+ reabsorptionNa+-H+ exchangeLuminal cell membraneMechanosensory functionNa+ absorptionTubular diameterLuminal diameterMouse kidneyEpithelial microvilliReabsorptionTubulesKidneyAutoregulatory mechanismNa+ uptakeHormonal systemsMiceActin cytoskeletonCell membraneTerminal webBending moment
2002
Formate-stimulated NaCl absorption in the proximal tubule is independent of the pendrin protein
Karniski LP, Wang T, Everett LA, Green ED, Giebisch G, Aronson PS. Formate-stimulated NaCl absorption in the proximal tubule is independent of the pendrin protein. American Journal Of Physiology. Renal Physiology 2002, 283: f952-f956. PMID: 12372770, DOI: 10.1152/ajprenal.00182.2002.Peer-Reviewed Original ResearchConceptsProximal tubulesChloride/formate exchangeNaCl reabsorptionChloride reabsorptionElectroneutral NaCl reabsorptionNaCl absorptionRole of pendrinFormate exchangePds-knockout mouseRecycling of formatePendrin proteinReabsorptionMicePendrinBrush border membrane vesiclesTubulesPresent studyChloride transportApical membrane
2001
Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron
Wang T, Hropot M, Aronson P, Giebisch G. Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron. American Journal Of Physiology. Renal Physiology 2001, 281: f1117-f1122. PMID: 11704563, DOI: 10.1152/ajprenal.2001.281.6.f1117.Peer-Reviewed Original ResearchConceptsLoop of HenleHOE 694Proximal tubulesAddition of ethylisopropylamilorideDistal convoluted tubuleBicarbonate reabsorptionConvoluted tubulesSitu microperfusionRat kidneySignificant inhibitionNHE isoformsHenlePredominant isoformTubulesNHE3Functional roleInhibitionNHE2Apical membraneIsoformsApical isoformKidneyMicroperfusionEthylisopropylamilorideNephron
1998
Tubule Function in Transgenic Mice
Wang T, Giebisch G. Tubule Function in Transgenic Mice. Nephron 1998, 6: 447-453. PMID: 9730661, DOI: 10.1159/000020554.Peer-Reviewed Original ResearchConceptsNa-HK-ATPaseK-ATPase expressionRenal tubule transportRenal electrolyte transportStrains of miceRenal transport functionsTransport functionColonic isoformH,K-ATPaseIsoforms NHE2Isoforms NHE1Tubule transportTubule functionTubule segmentsMouse modelTransgenic miceTransgenic knockoutTransport lesionKnockout modelsMiceKidney tubulesElectrolyte transportNull mutationTubulesEffect of metabolic acidosis on NaCl transport in the proximal tubule
Wang T, Egbert A, Aronson P, Giebisch G. Effect of metabolic acidosis on NaCl transport in the proximal tubule. American Journal Of Physiology 1998, 274: f1015-f1019. PMID: 9841491, DOI: 10.1152/ajprenal.1998.274.6.f1015.Peer-Reviewed Original ResearchConceptsMetabolic acidosisProximal tubulesNaCl reabsorptionRat proximal tubule segmentsAcidotic ratsNaCl transportNaCl absorptionLate proximal tubuleProximal tubule segmentsControl ratsCapillary perfusateBicarbonate absorptionNephron segmentsAcidosisTubule segmentsRatsRecent evidenceJclTubulesFormate exchangeReabsorptionDifferential regulation
1995
Stimulation of chloride transport by cAMP in rat proximal tubules
Wang T, Segal AS, Giebisch G, Aronson PS. Stimulation of chloride transport by cAMP in rat proximal tubules. American Journal Of Physiology 1995, 268: f204-f210. PMID: 7864157, DOI: 10.1152/ajprenal.1995.268.2.f204.Peer-Reviewed Original ResearchConceptsRat proximal tubuleProximal tubulesDibutyryl cAMPCapillary perfusateLate proximal tubuleProximal tubule cellsCl- channel blockerCl- channelsTranscellular Cl absorptionAddition of forskolinLuminal DIDSSensitive Cl- channelBasolateral membraneLuminal perfusateTubule cellsCAMP productionFluid absorptionCl absorptionMM ClPerfusateJclRecent evidenceLuminal membraneTubulesEffect of cAMPEffects of Glyburide on Renal Tubule Transport and Potassium-Channel Activity
Wang T, Wang W, Klein-Robbenhaar G, Giebisch G. Effects of Glyburide on Renal Tubule Transport and Potassium-Channel Activity. Kidney & Blood Pressure Research 1995, 18: 169-182. PMID: 7481068, DOI: 10.1159/000173914.Peer-Reviewed Original ResearchConceptsK channelsApical membraneInhibition of K secretionApical K channelsRenal tubule transportCortical collecting tubuleK channel activityPatch-clamp techniquePotassium channel activityEffect of glyburideTubule transportK secretionRenal clearanceElectrolyte excretionPotassium channelsTransport of NaAscending limbGlyburideInhibit transportK recyclingKaliuresisHenleMicroperfusionTubulesExcretion
1993
Renal bicarbonate reabsorption in the rat. IV. Bicarbonate transport mechanisms in the early and late distal tubule.
Wang T, Malnic G, Giebisch G, Chan Y. Renal bicarbonate reabsorption in the rat. IV. Bicarbonate transport mechanisms in the early and late distal tubule. Journal Of Clinical Investigation 1993, 91: 2776-2784. PMID: 8390489, PMCID: PMC443344, DOI: 10.1172/jci116519.Peer-Reviewed Original ResearchConceptsLate distal tubuleBicarbonate absorptionDistal tubulesTubule segmentsBicarbonate transportH-ATPaseH-K-ATPase inhibitorsRate of bicarbonate absorptionEthyl-isopropylamilorideFluid absorptionNa(+)-H+ exchangeK(+)-H+ exchangeEarly distal tubuleBicarbonate transport mechanismDistal tubule segmentsRenal bicarbonate reabsorptionLow-K dietLuminal bicarbonate concentrationBicarbonate reabsorptionBicarbonate secretionBicarbonate concentrationH+ secretionMicroperfusion experimentsTubulesK diet
1992
Effects of formate and oxalate on volume absorption in rat proximal tubule
Wang T, Giebisch G, Aronson PS. Effects of formate and oxalate on volume absorption in rat proximal tubule. American Journal Of Physiology 1992, 263: f37-f42. PMID: 1636743, DOI: 10.1152/ajprenal.1992.263.1.f37.Peer-Reviewed Original ResearchConceptsLuminal perfusateProximal tubulesCapillary perfusateLate proximal tubuleRat proximal tubuleHCO3- Ringer's solutionPhysiological rangePeritubular capillariesChannel blockersStandard Ringer solutionPerfusateFluid absorptionStimulatory effectNaCl absorptionRinger's solutionTubulesEffect of formateBlockers