2023
Live imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo
May D, Yun S, Gonzalez D, Park S, Chen Y, Lathrop E, Cai B, Xin T, Zhao H, Wang S, Gonzalez L, Cockburn K, Greco V. Live imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo. ELife 2023, 12: e83444. PMID: 36880644, PMCID: PMC10027315, DOI: 10.7554/elife.83444.Peer-Reviewed Original ResearchConceptsStem cell differentiationCell differentiationStem cell compartmentCompaction changesChromatin compaction statesDynamic transcriptional statesCell compartmentChromatin architectureCell cycle statusChromatin rearrangementNascent RNATranscriptional burstingTranscriptional statesLive imagingTissue contextGene expressionDifferentiating cellsGlobal remodelingIndividual cellsCycle statusStem cellsDifferentiation statusDifferentiationCellsMorphological changes
2013
dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary
Xuan T, Xin T, He J, Tan J, Gao Y, Feng S, He L, Zhao G, Li M. dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary. Developmental Biology 2013, 379: 167-181. PMID: 23624310, DOI: 10.1016/j.ydbio.2013.04.015.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosis Regulatory ProteinsCell DifferentiationDNA PrimersDrosophila melanogasterDrosophila ProteinsEpigenesis, GeneticFemaleGerm CellsHistone MethyltransferasesHistone-Lysine N-MethyltransferaseHistonesMethylationMicroscopy, FluorescenceOvaryReal-Time Polymerase Chain ReactionRNA InterferenceStatistics, NonparametricStem Cell NicheStem CellsUbiquitin-Protein LigasesConceptsGermline stem cellsGerm cell differentiationStem cell nicheCell differentiationDrosophila ovaryGSC maintenanceDrosophila ovarian germline stem cellsCell nicheGermline stem cell maintenanceOvarian germline stem cellsCell fate regulationStem cell maintenanceHistone H3K4 trimethylationE3 ubiquitin ligaseStem cellsSingle germ cellEscort cellsGSC lossGSC regulationChromatin remodelingMutant ovariesH3K4 methylationHistone modificationsFate regulationH3K4 trimethylation