2011
Biochemical and Morphological Effects of Hypoxic Environment on Human Embryonic Stem Cells in Long-Term Culture and Differentiating Embryoid Bodies
Lim H, Han J, Woo D, Kim S, Kim S, Kang H, Kim J. Biochemical and Morphological Effects of Hypoxic Environment on Human Embryonic Stem Cells in Long-Term Culture and Differentiating Embryoid Bodies. Molecules And Cells 2011, 31: 123-132. PMID: 21347709, PMCID: PMC3932683, DOI: 10.1007/s10059-011-0016-8.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsMild hypoxiaEmbryonic stem cellsEmbryoid bodiesLong-term cultureBrdU incorporationCell fate determinationDifferentiating embryoid bodiesExpression of markersCaspase-3 immunostainingStem cellsPercentage of cellsEmbryonic germ layersActive caspase-3Hematoxylineosin stainingMammalian reproductive tract
2009
Differential cytotoxic effects of mono-(2-ethylhexyl) phthalate on blastomere-derived embryonic stem cells and differentiating neurons
Lim C, Kim S, Ko D, Cho J, Jun J, An S, Han J, Kim J, Yoon Y. Differential cytotoxic effects of mono-(2-ethylhexyl) phthalate on blastomere-derived embryonic stem cells and differentiating neurons. Toxicology 2009, 264: 145-154. PMID: 19720108, DOI: 10.1016/j.tox.2009.08.015.Peer-Reviewed Original ResearchDifferentiation of Neural Progenitor Cells in a Microfluidic Chip‐Generated Cytokine Gradient
Park J, Kim S, Woo D, Lee E, Kim J, Lee S. Differentiation of Neural Progenitor Cells in a Microfluidic Chip‐Generated Cytokine Gradient. Stem Cells 2009, 27: 2646-2654. PMID: 19711444, DOI: 10.1002/stem.202.Peer-Reviewed Original ResearchConceptsCell typesNeural progenitorsHuman embryonic stem cellsEarly embryonic developmentStem cellsEmbryonic stem cellsCytokine gradientsEnriched populationNeural progenitor cellsPrimitive stem cellsEmbryonic developmentSignaling moleculesDiverse tissuesCell body clustersProgenitor cellsNeurite bundlesGrowth factorProgenitorsCell-friendly microenvironmentCellsDifferentiationExogenous cytokinesImportant roleBody clustersSpatial gradientsDifferentiation of Human Neural Progenitor Cells on PLGA Microfibers
Hwang C, Kim S, Kim J, Khademhosseini A, Lee S. Differentiation of Human Neural Progenitor Cells on PLGA Microfibers. 2009, 1: 1-2. DOI: 10.1109/nebc.2009.4967758.Peer-Reviewed Original ResearchHuman embryonic stem cellsNeural progenitor cellsEmbryoid bodiesMicrofluidic spinning systemPLGA microfibersNeural tissue regenerationEmbryonic stem cellsNeural tissue engineeringProgenitor cellsHuman neural progenitor cellsTissue engineeringNeuronal protein expressionNeural progenitor markersGlial fibrillary acidic proteinMicrofibersTissue regenerationNascent fibersPLGA fibersNeural progenitorsDifferentiated cellsCell differentiationProgenitor markersStem cellsGuidance cuesProtein expressionVitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells
Gil J, Woo D, Shim J, Kim S, You H, Park S, Paek S, Kim S, Kim J. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Letters 2009, 583: 561-567. PMID: 19162023, DOI: 10.1016/j.febslet.2008.12.061.Peer-Reviewed Original Research
2007
Directed differentiation of human embryonic stem cells towards a pancreatic cell fate
Shim J, Kim S, Woo D, Kim S, Oh C, McKay R, Kim J. Directed differentiation of human embryonic stem cells towards a pancreatic cell fate. Diabetologia 2007, 50: 1228-1238. PMID: 17457565, DOI: 10.1007/s00125-007-0634-z.Peer-Reviewed Original ResearchMeSH KeywordsActivinsAnimalsCell Culture TechniquesCell DifferentiationDiabetes Mellitus, ExperimentalEmbryonic Stem CellsGene Expression RegulationHomeodomain ProteinsHumansMaleMiceMice, Inbred BALB CMice, NudePancreasReverse Transcriptase Polymerase Chain ReactionStem Cell TransplantationTrans-ActivatorsTransplantation, HeterologousTretinoinConceptsHuman embryonic stem cellsPdx1-positive cellsForkhead box protein A2Embryonic stem cellsEndodermal cellsCaudal-type homeobox transcription factor 2Differentiation of hESCsCell typesPancreatic cell fateRetinoic acidStem cellsMature cell typesEmbryonic germ layersEmbryoid body formationPancreatic cell typesBox protein A2Transcription factor 2Vivo pancreatic developmentEpigenetic signalsCell fateEctodermal differentiationDuodenal homeobox 1Pancreatic fateGene 17Gene expressionComparative Analysis of the Developmental Competence of Three Human Embryonic Stem Cell Lines in Vitro
Kim S, Kim B, Gil J, Kim S, Kim J. Comparative Analysis of the Developmental Competence of Three Human Embryonic Stem Cell Lines in Vitro. Molecules And Cells 2007, 23: 49-56. PMID: 17464211, DOI: 10.1016/s1016-8478(23)07388-0.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsHESC linesHuman embryonic stem cell linesEmbryonic stem cell linesPrimordial germ cellsDevelopmental potentialEmbryonic stem cellsEmbryonic germ layersEmbryoid body formationSpecific cell typesDevelopmental competenceStem cell linesCell replacement therapyExtraembryonic tissuesStem cell technologyGerm layersTerminal differentiationGerm cellsBody formationPluripotency markersCell typesNeuronal cellsTelomerase activityStem cellsHSF6
2006
Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells
Kim B, Kim S, Shim J, Woo D, Gil J, Kim S, Kim J. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells. FEBS Letters 2006, 580: 5869-5874. PMID: 17027979, DOI: 10.1016/j.febslet.2006.09.053.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsVascular endothelial growth factorGerm layer formationEmbryonic stem cellsEmbryoid bodiesEndothelial growth factorNeurogenic effectsGrowth factorStem cellsEffects of VEGFNeuroectodermal genesVascular endothelial cellsHESC differentiationNeural rosettesDopaminergic neuronsGene expressionNeural progenitorsTerminal differentiationVEGF treatmentPotent mitogenEndothelial cellsNeuroectodermal differentiationDifferentiationCellsExpressionNonylphenol and Octylphenol-Induced Apoptosis in Human Embryonic Stem Cells Is Related to Fas-Fas Ligand Pathway
Kim S, Kim B, Shim J, Gil J, Yoon Y, Kim J. Nonylphenol and Octylphenol-Induced Apoptosis in Human Embryonic Stem Cells Is Related to Fas-Fas Ligand Pathway. Toxicological Sciences 2006, 94: 310-321. PMID: 16984955, DOI: 10.1093/toxsci/kfl114.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsUndifferentiated hES cellsEmbryonic stem cellsHES cellsNeural progenitor cellsEarly embryonic developmentStem cellsProgenitor cellsToxic stress responseChemical-induced apoptosisFas ligand protein expressionFas-Fas ligand pathwayCell replacement therapyEmbryonic developmentCaspase activationES cellsCaspase-8Stress responseDevelopmental stagesDNA fragmentationCell typesFas-FasL interactionCommon environmental contaminantsApoptosisProtein expression