2019
Macrophage‐Derived microRNA‐155 Increases in Obesity and Influences Adipocyte Metabolism by Targeting Peroxisome Proliferator‐Activated Receptor Gamma
Tryggestad J, Teague A, Sparling D, Jiang S, Chernausek S. Macrophage‐Derived microRNA‐155 Increases in Obesity and Influences Adipocyte Metabolism by Targeting Peroxisome Proliferator‐Activated Receptor Gamma. Obesity 2019, 27: 1856-1864. PMID: 31531958, PMCID: PMC6832842, DOI: 10.1002/oby.22616.Peer-Reviewed Original ResearchConceptsAdipose tissue macrophagesMiR-155Mesenchymal stem cellsStem cellsPeripheral blood mononuclear cellsPeroxisome proliferator-activated receptor gammaBlood mononuclear cellsImpact of obesityProliferator-activated receptor gammaInfluence adipocyte metabolismMiR-130b expressionCell type-specific fashionIncreased 1.8-foldType-specific fashionPPARG mRNA expressionWestern blot analysisMononuclear cellsPolymerase chain reactionQuantitative polymerase chain reactionMiRNA expressionHuman mesenchymal stem cellsTissue macrophagesDifferentiated 3T3-L1 adipocytesMRNA expressionObesity
2016
Influence of gestational diabetes mellitus on human umbilical vein endothelial cell miRNA.
Tryggestad J, Vishwanath A, Jiang S, Mallappa A, Teague A, Takahashi Y, Thompson D, Chernausek S. Influence of gestational diabetes mellitus on human umbilical vein endothelial cell miRNA. Clinical Science 2016, 130: 1955-67. PMID: 27562513, PMCID: PMC5516927, DOI: 10.1042/cs20160305.Peer-Reviewed Original ResearchConceptsGestational diabetes mellitusMiR-130bMiR-let-7a-5pMiR-148aLevel of miR-130bHuman umbilical vein endothelial cellsMiR-148a-3pMiR-30c-5pMiR-148a mimicsInfants of womenMiR-130b-3pMiRNA expressionMiR-452MiR-126Influence of gestational diabetes mellitusDiabetes mellitusTarget protein expressionProtein expressionTarget protein abundanceMiRNAsMonths of ageUmbilical vein endothelial cellsMiRNA speciesQuantitative PCRFetal tissues