1995
Downregulation of Lck-mediated signal transduction by tip of herpesvirus saimiri
Jung J, Lang S, Jun T, Roberts T, Veillette A, Desrosiers R. Downregulation of Lck-mediated signal transduction by tip of herpesvirus saimiri. Journal Of Virology 1995, 69: 7814-7822. PMID: 7494293, PMCID: PMC189725, DOI: 10.1128/jvi.69.12.7814-7822.1995.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCell LineCell Line, TransformedCloning, MolecularDNA PrimersElectrophoresis, Polyacrylamide GelGene ExpressionHerpesvirus 2, SaimiriineHumansLymphocyte Specific Protein Tyrosine Kinase p56(lck)Molecular Sequence DataMolecular WeightPhosphoproteinsPlasmidsPolymerase Chain ReactionRecombinant Fusion ProteinsSignal TransductionSrc-Family KinasesT-LymphocytesTumor Cells, CulturedViral ProteinsConceptsExpression of tipSignal transductionTyrosine phosphorylationT cell signal transductionCellular signal transductionCellular tyrosine phosphorylationHuman Jurkat T cell lineJurkat T cell lineJurkat T cellsInhibition of LckSH3 domainLckTransductionHerpesvirus saimiriFibroblast cellsPhosphorylationStable complexesSurface expressionBinding assaysComplex formationExpressionCellsAnti-CD3 stimulationT cellsB cellsIdentification of Lck-binding Elements in Tip of Herpesvirus Saimiri (∗)
Jung J, Lang S, Friedrich U, Jun T, Roberts T, Desrosiers R, Biesinger B. Identification of Lck-binding Elements in Tip of Herpesvirus Saimiri (∗). Journal Of Biological Chemistry 1995, 270: 20660-20667. PMID: 7544793, DOI: 10.1074/jbc.270.35.20660.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCell LineChlorocebus aethiopsDNA PrimersHerpesvirus 2, SaimiriineLymphocyte Specific Protein Tyrosine Kinase p56(lck)Molecular Sequence DataMutagenesis, Site-DirectedPeptide FragmentsPhosphoproteinsPhosphoserinePhosphothreoninePhosphotyrosinePoint MutationPolymerase Chain ReactionProtein-Tyrosine KinasesRatsRecombinant ProteinsSequence Homology, Amino AcidSpodopteraStructure-Activity RelationshipTransfectionTyrosineViral ProteinsConceptsC-terminal SrcCellular protein kinasesBinding of sequencesInteraction of TipHerpesvirus saimiriLck kinase activityCOS-1 cellsRat-1 cell lineLck functionPutative SH3Cellular proteinsSH3 bindingProtein kinaseCarboxyl terminusLck kinaseVivo associationKinase activityMutational analysisFusion proteinLckAmino acidsKinaseHuman T cellsProteinCell lines
1993
Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus
Lang S, Weeger M, Stahl-Hennig C, Coulibaly C, Hunsmann G, Müller J, Müller-Hermelink H, Fuchs D, Wachter H, Daniel M. Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus. Journal Of Virology 1993, 67: 902-912. PMID: 8380472, PMCID: PMC237444, DOI: 10.1128/jvi.67.2.902-912.1993.Peer-Reviewed Original ResearchConceptsVpr variantsTAA stop codonVpr geneATG start codonSite-specific mutagenesisVpr mutant virusSignificant selective pressureImportance of VprStart codonSelective pressureVpr mutantsStop codonGenesPathogenic molecular cloneMolecular cloneCell linesVprTest animalsCEMx174 cell lineCodonNef geneSimilar kineticsVirus replicationReversionStop signal