1997
Association of simian immunodeficiency virus Nef with cellular serine/threonine kinases is dispensable for the development of AIDS in rhesus macaques
Lang S, Lafrate A, Stahl-Hennig C, Kuhn E, Nisslein T, Kaup F, Haupt M, Hunsmann G, Skowronski J, Kirchhoff F. Association of simian immunodeficiency virus Nef with cellular serine/threonine kinases is dispensable for the development of AIDS in rhesus macaques. Nature Medicine 1997, 3: 860-865. PMID: 9256276, DOI: 10.1038/nm0897-860.Peer-Reviewed Original ResearchConceptsCellular serine/threonine kinaseSerine/threonine kinaseThreonine kinaseTyrosine kinase SrcPutative SH3Simian immunodeficiency virus NefKinase SrcMutant formsEfficient replicationKinaseInduction of AIDSSimian immunodeficiency virusNef geneCD4 downregulationSIVmac NefRhesus macaquesRapid deathNef sequencesSH3NefSrcGenesDomainDifferent time pointsPathogenicity
1995
Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys
Du Z, Lang S, Sasseville V, Lackner A, Ilyinskii P, Daniel M, Jung J, Desrosiers R. Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys. Cell 1995, 82: 665-674. PMID: 7664345, DOI: 10.1016/0092-8674(95)90038-1.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAllelesAmino Acid SequenceAnimalsBase SequenceDNA PrimersDNA, ViralGene Products, envGene Products, nefGenes, nefLymphocyte ActivationMacaca mulattaMiceMolecular Sequence DataPhagocytesPhenotypePhosphorylationRetroviridae Proteins, OncogenicSignal TransductionSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusTransformation, GeneticTyrosineViral Fusion ProteinsVirus ReplicationConceptsAcute diseasePeripheral blood mononuclear cell culturesBlood mononuclear cell culturesMononuclear cell culturesT lymphocyte activationSevere diarrheaLymphoid proliferationsGastrointestinal tractNef allelesMacaque monkeysLymphocyte activationCellular activationSIVmac239NefDiseaseActivationMonkeysCell culturesImportant determinantCellsNIH 3T3 cellsTyrosine phosphorylationCotransfected COS cellsCOS cellsDiarrhea
1993
Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus
Lang S, Weeger M, Stahl-Hennig C, Coulibaly C, Hunsmann G, Müller J, Müller-Hermelink H, Fuchs D, Wachter H, Daniel M. Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus. Journal Of Virology 1993, 67: 902-912. PMID: 8380472, PMCID: PMC237444, DOI: 10.1128/jvi.67.2.902-912.1993.Peer-Reviewed Original ResearchConceptsVpr variantsTAA stop codonVpr geneATG start codonSite-specific mutagenesisVpr mutant virusSignificant selective pressureImportance of VprStart codonSelective pressureVpr mutantsStop codonGenesPathogenic molecular cloneMolecular cloneCell linesVprTest animalsCEMx174 cell lineCodonNef geneSimilar kineticsVirus replicationReversionStop signal