2004
Impact of Nef-Mediated Downregulation of Major Histocompatibility Complex Class I on Immune Response to Simian Immunodeficiency Virus
Swigut T, Alexander L, Morgan J, Lifson J, Mansfield K, Lang S, Johnson R, Skowronski J, Desrosiers R. Impact of Nef-Mediated Downregulation of Major Histocompatibility Complex Class I on Immune Response to Simian Immunodeficiency Virus. Journal Of Virology 2004, 78: 13335-13344. PMID: 15542684, PMCID: PMC525019, DOI: 10.1128/jvi.78.23.13335-13344.2004.Peer-Reviewed Original ResearchConceptsSimian immunodeficiency virusHuman immunodeficiency virusImmunodeficiency virusWeeks postinfectionMajor Histocompatibility Complex Class I DownregulationClass IMajor histocompatibility complex class IHistocompatibility complex class IComplex class IMHC class INef gene productParental wild-type virusImmune responseLymphocyte activationWild-type virusNef functionCD4 downregulationFunctional activityMHC downregulationDownregulationResponse-4VirusMonkeysPostinfectionNef
2000
Simian Immunodeficiency Virus Containing Mutations in N-Terminal Tyrosine Residues and in the PxxP Motif in Nef Replicates Efficiently in Rhesus Macaques
Carl S, Iafrate A, Lang S, Stolte N, Stahl-Hennig C, Mätz-Rensing K, Fuchs D, Skowronski J, Kirchhoff F. Simian Immunodeficiency Virus Containing Mutations in N-Terminal Tyrosine Residues and in the PxxP Motif in Nef Replicates Efficiently in Rhesus Macaques. Journal Of Virology 2000, 74: 4155-4164. PMID: 10756028, PMCID: PMC111930, DOI: 10.1128/jvi.74.9.4155-4164.2000.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCD4 AntigensCell Line, TransformedCOS CellsDown-RegulationGene Products, nefHistocompatibility Antigens Class IHumansMacaca mulattaMutagenesisP21-Activated KinasesPhosphorylationProtein Serine-Threonine KinasesSimian immunodeficiency virusSrc Homology DomainsTyrosineViral LoadVirus ReplicationConceptsAbility of NefTyrosine-based endocytosis signalTyrosine residuesHigh viral loadCellular signal transductionTyrosine kinase SrcNef functionViral loadEndocytosis signalEndocytic machineryPXXP motifN-terminal tyrosine residueCell surface expressionKinase SrcKinase assaysSignal transductionProline residuesClass I major histocompatibility complexN-terminal tyrosineI major histocompatibility complexLigand domainCombined mutationsNef interactionsMajor histocompatibility complexMutations
1999
The Acidic Region and Conserved Putative Protein Kinase C Phosphorylation Site in Nef Are Important for SIV Replication in Rhesus Macaques
Carl S, Iafrate A, Lang S, Stahl-Hennig C, Kuhn E, Fuchs D, Mätz-Rensing K, Haaft P, Heeney J, Skowronski J, Kirchhoff F. The Acidic Region and Conserved Putative Protein Kinase C Phosphorylation Site in Nef Are Important for SIV Replication in Rhesus Macaques. Virology 1999, 257: 138-155. PMID: 10208928, DOI: 10.1006/viro.1999.9645.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCD4 AntigensCOS CellsGene Products, nefHistocompatibility Antigens Class IHIV-1Hydrogen-Ion ConcentrationMacaca mulattaMolecular Sequence DataMutagenesis, Site-DirectedNef Gene Products, Human Immunodeficiency VirusPhosphorylationProtein Kinase CSimian immunodeficiency virusSurface PropertiesVirus ReplicationConceptsProtein kinase C phosphorylation sitesKinase C phosphorylation sitesC phosphorylation sitesPhosphorylation sitesPutative protein kinase C phosphorylation sitePotential protein kinase C phosphorylation sitesAcidic regionWild-type activityPKC phosphorylation sitesJurkat T cellsSignal transductionAbility of NefReplication assaysFunctional relevanceCell culture systemMutant virusNef functionPathogenic SIVmac239 cloneAcidic chargeReduced infectivityReplicationCulture systemNefRapid reversionRhesus macaques