2004
Impact of Nef-Mediated Downregulation of Major Histocompatibility Complex Class I on Immune Response to Simian Immunodeficiency Virus
Swigut T, Alexander L, Morgan J, Lifson J, Mansfield K, Lang S, Johnson R, Skowronski J, Desrosiers R. Impact of Nef-Mediated Downregulation of Major Histocompatibility Complex Class I on Immune Response to Simian Immunodeficiency Virus. Journal Of Virology 2004, 78: 13335-13344. PMID: 15542684, PMCID: PMC525019, DOI: 10.1128/jvi.78.23.13335-13344.2004.Peer-Reviewed Original ResearchConceptsSimian immunodeficiency virusHuman immunodeficiency virusImmunodeficiency virusWeeks postinfectionMajor Histocompatibility Complex Class I DownregulationClass IMajor histocompatibility complex class IHistocompatibility complex class IComplex class IMHC class INef gene productParental wild-type virusImmune responseLymphocyte activationWild-type virusNef functionCD4 downregulationFunctional activityMHC downregulationDownregulationResponse-4VirusMonkeysPostinfectionNef
1999
The Acidic Region and Conserved Putative Protein Kinase C Phosphorylation Site in Nef Are Important for SIV Replication in Rhesus Macaques
Carl S, Iafrate A, Lang S, Stahl-Hennig C, Kuhn E, Fuchs D, Mätz-Rensing K, Haaft P, Heeney J, Skowronski J, Kirchhoff F. The Acidic Region and Conserved Putative Protein Kinase C Phosphorylation Site in Nef Are Important for SIV Replication in Rhesus Macaques. Virology 1999, 257: 138-155. PMID: 10208928, DOI: 10.1006/viro.1999.9645.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCD4 AntigensCOS CellsGene Products, nefHistocompatibility Antigens Class IHIV-1Hydrogen-Ion ConcentrationMacaca mulattaMolecular Sequence DataMutagenesis, Site-DirectedNef Gene Products, Human Immunodeficiency VirusPhosphorylationProtein Kinase CSimian immunodeficiency virusSurface PropertiesVirus ReplicationConceptsProtein kinase C phosphorylation sitesKinase C phosphorylation sitesC phosphorylation sitesPhosphorylation sitesPutative protein kinase C phosphorylation sitePotential protein kinase C phosphorylation sitesAcidic regionWild-type activityPKC phosphorylation sitesJurkat T cellsSignal transductionAbility of NefReplication assaysFunctional relevanceCell culture systemMutant virusNef functionPathogenic SIVmac239 cloneAcidic chargeReduced infectivityReplicationCulture systemNefRapid reversionRhesus macaques
1997
Association of simian immunodeficiency virus Nef with cellular serine/threonine kinases is dispensable for the development of AIDS in rhesus macaques
Lang S, Lafrate A, Stahl-Hennig C, Kuhn E, Nisslein T, Kaup F, Haupt M, Hunsmann G, Skowronski J, Kirchhoff F. Association of simian immunodeficiency virus Nef with cellular serine/threonine kinases is dispensable for the development of AIDS in rhesus macaques. Nature Medicine 1997, 3: 860-865. PMID: 9256276, DOI: 10.1038/nm0897-860.Peer-Reviewed Original ResearchConceptsCellular serine/threonine kinaseSerine/threonine kinaseThreonine kinaseTyrosine kinase SrcPutative SH3Simian immunodeficiency virus NefKinase SrcMutant formsEfficient replicationKinaseInduction of AIDSSimian immunodeficiency virusNef geneCD4 downregulationSIVmac NefRhesus macaquesRapid deathNef sequencesSH3NefSrcGenesDomainDifferent time pointsPathogenicity
1995
Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys
Du Z, Lang S, Sasseville V, Lackner A, Ilyinskii P, Daniel M, Jung J, Desrosiers R. Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys. Cell 1995, 82: 665-674. PMID: 7664345, DOI: 10.1016/0092-8674(95)90038-1.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAllelesAmino Acid SequenceAnimalsBase SequenceDNA PrimersDNA, ViralGene Products, envGene Products, nefGenes, nefLymphocyte ActivationMacaca mulattaMiceMolecular Sequence DataPhagocytesPhenotypePhosphorylationRetroviridae Proteins, OncogenicSignal TransductionSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusTransformation, GeneticTyrosineViral Fusion ProteinsVirus ReplicationConceptsAcute diseasePeripheral blood mononuclear cell culturesBlood mononuclear cell culturesMononuclear cell culturesT lymphocyte activationSevere diarrheaLymphoid proliferationsGastrointestinal tractNef allelesMacaque monkeysLymphocyte activationCellular activationSIVmac239NefDiseaseActivationMonkeysCell culturesImportant determinantCellsNIH 3T3 cellsTyrosine phosphorylationCotransfected COS cellsCOS cellsDiarrhea