2004
Role of dipeptidyl peptidase IV in regulating activity of Na+/H+ exchanger isoform NHE3 in proximal tubule cells
Girardi AC, Knauf F, Demuth HU, Aronson PS. Role of dipeptidyl peptidase IV in regulating activity of Na+/H+ exchanger isoform NHE3 in proximal tubule cells. American Journal Of Physiology - Cell Physiology 2004, 287: c1238-c1245. PMID: 15213057, DOI: 10.1152/ajpcell.00186.2004.Peer-Reviewed Original ResearchConceptsProximal tubule cellsDPPIV inhibitorsDipeptidyl peptidase IVTubule cellsNHE3 activityOpossum kidney proximal tubule cellsKidney proximal tubule cellsNHE3 surface expressionPeptidase IVAdditive inhibitory effectEffects of genisteinForskolin/IBMXTyrosine kinase inhibitor genisteinSpecific competitive inhibitorPKA inhibitor HKinase inhibitor genisteinP32/98Activation of PKAInhibitory effectRenal brush border membraneBrush border membraneSurface expressionProtein kinase pathwayNHE3Inhibitor genistein
1997
Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat.
Rutherford PA, Pizzonia JH, Biemesderfer D, Abu-Alfa A, Reilly R, Aronson PS. Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat. Nephron 1997, 5: 490-7. PMID: 9438178.Peer-Reviewed Original ResearchConceptsNa-H exchangeNa-H exchangerBlood cellsRat kidneyNa-Li countertransportNHE1 protein expressionExpression of NHE1Diabetic nephropathyCountertransport activityIsoform-specific antibodiesMonoclonal antibodiesExchanger isoforms NHE1NHE3 expressionRenal brush border membraneProtein expressionBrush border membraneKidneyNHE isoformsRabbitsNHE3Blood cell membranesBrush border membrane fractionRatsIsoforms NHE1Antibodies
1996
Role of NHE3 in Mediating Renal Brush Border Na+-H+ Exchange ADAPTATION TO METABOLIC ACIDOSIS*
Wu M, Biemesderfer D, Giebisch G, Aronson P. Role of NHE3 in Mediating Renal Brush Border Na+-H+ Exchange ADAPTATION TO METABOLIC ACIDOSIS*. Journal Of Biological Chemistry 1996, 271: 32749-32752. PMID: 8955109, DOI: 10.1074/jbc.271.51.32749.Peer-Reviewed Original ResearchConceptsMetabolic acidosisControl ratsBrush border membraneRenal brush border vesiclesNHE3 proteinBrush border vesiclesBorder membraneBrush borderNHE3 protein expressionRole of NHE3Western blot analysisNormal ratsAcidosisRatsAcidotic ratsRenal brush border membranePharmacologic inhibitorsProtein expressionExchange activityInhibitor sensitivityNHE3Renal brush borderBlot analysis
1993
NHE3: a Na+/H+ exchanger isoform of renal brush border
Biemesderfer D, Pizzonia J, Abu-Alfa A, Exner M, Reilly R, Igarashi P, Aronson PS. NHE3: a Na+/H+ exchanger isoform of renal brush border. American Journal Of Physiology 1993, 265: f736-f742. PMID: 8238556, DOI: 10.1152/ajprenal.1993.265.5.f736.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceDNA PrimersFluorescent Antibody TechniqueImmunoblottingImmunohistochemistryKidneyKidney CortexKidney Tubules, ProximalL CellsMiceMicrovilliMolecular Sequence DataMolecular WeightPolymerase Chain ReactionRabbitsRecombinant ProteinsSodium-Hydrogen ExchangersTransfectionConceptsBrush borderRabbit kidneyBrush border membraneProximal tubule cellsRenal brush borderRenal proximal tubulesWestern blot analysisImmune serumProximal tubulesTubule cellsGuinea pigsImmunocytochemical studyRenal brush border membraneKidneyTranscellular reabsorptionExchanger isoformsNHE3Blot analysisFusion proteinSpecific reactivityBasolateral membraneExchanger geneCellsProtein