2005
Immunolocalization of anion transporter Slc26a7 in mouse kidney
Dudas PL, Mentone S, Greineder CF, Biemesderfer D, Aronson PS. Immunolocalization of anion transporter Slc26a7 in mouse kidney. American Journal Of Physiology. Renal Physiology 2005, 290: f937-f945. PMID: 16263805, DOI: 10.1152/ajprenal.00197.2004.Peer-Reviewed Original ResearchConceptsThick ascending limbMouse kidneyProximal tubulesAscending limbThick ascending limb cellsLoop of HenleProximal tubule cellsSimilar staining patternExpression of SLC26A7Recent studiesTubule cellsNull miceNephron segmentsRat kidneyKidneyDifferent epitopesStaining patternMonoclonal antibodiesAntibodiesApical membrane transportersLimb cellsCOS-7 cellsImmunofluorescence microscopyLimbBasolateral surface
1998
Immunochemical characterization of Na+/H+exchanger isoform NHE4
Pizzonia J, Biemesderfer D, Abu-Alfa A, Wu M, Exner M, Isenring P, Igarashi P, Aronson P. Immunochemical characterization of Na+/H+exchanger isoform NHE4. American Journal Of Physiology 1998, 275: f510-f517. PMID: 9755122, DOI: 10.1152/ajprenal.1998.275.4.f510.Peer-Reviewed Original ResearchConceptsSubcellular localizationFusion proteinWestern blot analysisBlot analysisBroad tissue distributionBasolateral membraneTransport proteinsMembrane fractionProteinEpithelial cellsSkeletal muscleGradient centrifugationTissue distributionPercoll gradient centrifugationCellsGastric glandsNHE4MembraneImmunochemical characterizationMammalianLocalizationRat tissuesImmunochemical techniquesMonoclonal antibodiesPolypeptideMembrane Topology of NHE3 EPITOPES WITHIN THE CARBOXYL-TERMINAL HYDROPHILIC DOMAIN ARE EXOPLASMIC*
Biemesderfer D, DeGray B, Aronson P. Membrane Topology of NHE3 EPITOPES WITHIN THE CARBOXYL-TERMINAL HYDROPHILIC DOMAIN ARE EXOPLASMIC*. Journal Of Biological Chemistry 1998, 273: 12391-12396. PMID: 9575193, DOI: 10.1074/jbc.273.20.12391.Peer-Reviewed Original ResearchConceptsExchanger Isoform NHE3Membrane vesiclesAbsence of detergentPlasma membraneBorder membrane vesiclesCarboxyl-terminal hydrophilic domainBrush border membrane vesiclesCarboxyl-terminal domainMembrane topologyCytoskeletal effectorsCarboxyl terminusHigh-resolution immunocytochemistryExoplasmic surfaceCytoplasmic surfaceImmunoprecipitation studiesTransporter activityAmino acidsUltrathin cryosectionsImmunoblot analysisMembrane orientationHydrophilic domainsVesiclesMonoclonal antibodiesIntact microvilliGold technique
1997
Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney
Biemesderfer D, Rutherford PA, Nagy T, Pizzonia JH, Abu-Alfa AK, Aronson PS. Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney. American Journal Of Physiology 1997, 273: f289-f299. PMID: 9277590, DOI: 10.1152/ajprenal.1997.273.2.f289.Peer-Reviewed Original ResearchConceptsProximal tubulesRat kidneyMonoclonal antibodiesLoop of HenleHigh-resolution immunocytochemical studiesProximal tubule cellsPrevious immunochemical studiesIsoform-specific monoclonal antibodiesNeonatal rat kidneyTubule cellsImmunocytochemical studyThick limbImmunocytochemical techniquesKidneyLAP cellsRenal epitheliumNHE3Immunochemical studiesHigh-resolution immunocytochemical techniqueAdultsLater stagesNephron developmentExpression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat.
Rutherford PA, Pizzonia JH, Biemesderfer D, Abu-Alfa A, Reilly R, Aronson PS. Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat. Nephron 1997, 5: 490-7. PMID: 9438178.Peer-Reviewed Original ResearchConceptsNa-H exchangeNa-H exchangerBlood cellsRat kidneyNa-Li countertransportNHE1 protein expressionExpression of NHE1Diabetic nephropathyCountertransport activityIsoform-specific antibodiesMonoclonal antibodiesExchanger isoforms NHE1NHE3 expressionRenal brush border membraneProtein expressionBrush border membraneKidneyNHE isoformsRabbitsNHE3Blood cell membranesBrush border membrane fractionRatsIsoforms NHE1Antibodies