Pleiotropic Insulin Signals are Engaged by Multisite Phosphorylation of IRS-1
Sun X, Crimmins D, Myers M, Miralpeix M, White M. Pleiotropic Insulin Signals are Engaged by Multisite Phosphorylation of IRS-1. Molecular And Cellular Biology 1993, 13: 7418-7428. DOI: 10.1128/mcb.13.12.7418-7428.1993.Peer-Reviewed Original ResearchSrc homology 2IRS-1SH2 domainInsulin signalingPotential tyrosine phosphorylation sitesTyrosine residuesSrc homology 2 domainSrc homology 2 proteinAmino-terminal SH2 domainInsulin stimulationPhosphorylation of tyrosine residuesTyrosine phosphorylation sitesMultisite docking proteinInsulin signal transmissionInsulin receptor substrateInsulin receptor kinaseInsulin-stimulated phosphorylationDownstream regulatory elementsPurified insulin receptorYMXM motifsActivating insulin receptor kinaseDocking proteinMultisite phosphorylationPhosphorylation sitesRegulatory elementsIRS-1 is a common element in insulin and insulin-like growth factor-I signaling to the phosphatidylinositol 3'-kinase
Myers M, Sun X, Cheatham B, Jachna B, Glasheen E, Backer J, White M. IRS-1 is a common element in insulin and insulin-like growth factor-I signaling to the phosphatidylinositol 3'-kinase. Endocrinology 1993, 132: 1421-1430. DOI: 10.1210/en.132.4.1421.Peer-Reviewed Original ResearchPtdIns 3-kinaseTyrosine phosphorylation of IRS-1PtdIns 3-kinase activityPhosphorylation of IRS-1Insulin-like growth factor-IIRS-1Phosphatidylinositol 3-kinaseBinding to p85Increased tyrosine phosphorylation of IRS-1Insulin receptorStimulation of intact cellsPurified insulin receptorIncreased tyrosine phosphorylationSites in vitroChinese hamster ovary cellsTreatment of cellsInsulin-like growth factor-I signalingTyrosine phosphorylationHamster ovary cellsGrowth factor-IPtdInsCytosolic proteinsInsulin stimulationIntact cellsQuiescent cells