Insulin-Stimulated Oocyte Maturation Requires Insulin Receptor Substrate 1 and Interaction with the SH2 Domains of Phosphatidylinositol 3-Kinase
Chuang L, Myers M, Backer J, Shoelson S, White M, Birnbaum M, Kahin C. Insulin-Stimulated Oocyte Maturation Requires Insulin Receptor Substrate 1 and Interaction with the SH2 Domains of Phosphatidylinositol 3-Kinase. Molecular And Cellular Biology 1993, 13: 6653-6660. DOI: 10.1128/mcb.13.11.6653-6660.1993.Peer-Reviewed Original ResearchSrc homology 2 domainSrc homology 2PtdIns 3-kinaseSrc homology 2 domains of p85Glutathione S-transferase fusion proteinS-transferase fusion proteinDomains of p85IRS-1Ras-GAPP85 subunit of PtdIns 3-kinaseFusion proteinActivation of PtdIns 3-kinasePtdIns 3-kinase activitySubstrate 1Phosphorylation of IRS-1IRS-1 associationInsulin receptor substrate 1Insulin-stimulated phosphatidylinositolIRS-1 proteinPhosphorylated IRS-1Progesterone-induced oocyte maturationP85 subunitOocyte maturationInsulin signalingResponse to hormonal stimulationIRS-1 is a common element in insulin and insulin-like growth factor-I signaling to the phosphatidylinositol 3'-kinase
Myers M, Sun X, Cheatham B, Jachna B, Glasheen E, Backer J, White M. IRS-1 is a common element in insulin and insulin-like growth factor-I signaling to the phosphatidylinositol 3'-kinase. Endocrinology 1993, 132: 1421-1430. DOI: 10.1210/en.132.4.1421.Peer-Reviewed Original ResearchPtdIns 3-kinaseTyrosine phosphorylation of IRS-1PtdIns 3-kinase activityPhosphorylation of IRS-1Insulin-like growth factor-IIRS-1Phosphatidylinositol 3-kinaseBinding to p85Increased tyrosine phosphorylation of IRS-1Insulin receptorStimulation of intact cellsPurified insulin receptorIncreased tyrosine phosphorylationSites in vitroChinese hamster ovary cellsTreatment of cellsInsulin-like growth factor-I signalingTyrosine phosphorylationHamster ovary cellsGrowth factor-IPtdInsCytosolic proteinsInsulin stimulationIntact cellsQuiescent cells