1996
Na-K-ATPase alpha-isoform expression in heart and vascular endothelia: cellular and developmental regulation
Zahler R, Sun W, Ardito T, Kashgarian M. Na-K-ATPase alpha-isoform expression in heart and vascular endothelia: cellular and developmental regulation. American Journal Of Physiology 1996, 270: c361-c371. PMID: 8772464, DOI: 10.1152/ajpcell.1996.270.1.c361.Peer-Reviewed Original ResearchConceptsCardiac conduction systemAlpha 2Alpha 1Alpha 3Vascular endotheliumConduction systemHigh ouabain affinityNa-K-ATPase isoformsDevelopmental regulationAlpha 3 expressionImportant species differencesNeonatal rat myocardiumIsoform patternNeonatal ratsPapillary musclesDistinct physiological rolesSmooth muscleImmunohistochemical analysisConduction tissueVentricular myocardiumOuabain affinityRat myocardiumNormal endotheliumRat heartT-tubule system
1995
A unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator
Ameen N, Ardito T, Kashgarian M, Marino C. A unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator. Gastroenterology 1995, 108: 1016-1023. PMID: 7535272, DOI: 10.1016/0016-5085(95)90198-1.Peer-Reviewed Original ResearchConceptsProximal small intestineCrypt epithelial cellsVillus cellsSmall intestineHuman proximal small intestineCystic fibrosis transmembrane conductance regulatorEpithelial cellsSurface marker expressionFibrosis transmembrane conductance regulatorBrush border sucraseTransmembrane conductance regulatorIntestinal villus cellsBACKGROUND/Marker antibodiesMarker expressionUnique subsetVillus enterocytesConductance regulatorIntestineLactase expressionBrush border
1989
Aldosterone induction of electrogenic sodium transport in the apical membrane vesicles of rat distal colon*
Rajendran V, Kashgarian M, Binder H. Aldosterone induction of electrogenic sodium transport in the apical membrane vesicles of rat distal colon*. Journal Of Biological Chemistry 1989, 264: 18638-18644. PMID: 2553716, DOI: 10.1016/s0021-9258(18)51514-3.Peer-Reviewed Original ResearchConceptsDistal colonRat distal colonNa-H exchangeElectrogenic sodium transportSodium transportExperimental ratsMicroM amilorideAmiloride-sensitive electrogenic sodium transportNormal rat distal colonEffects of aldosteroneElectroneutral sodium absorptionSodium concentrationApical membrane vesiclesAnalogues of amilorideVoltage-clamp conditionsDietary sodiumNormal ratsAldosterone inductionSodium absorptionColonComparable inhibitionRatsAmilorideAldosteronePotent inhibitor
1988
[36] Preparation and use of monoclonal antibodies to Na+,K+-ATPase
Kashgarian M, Biemesderfer D. [36] Preparation and use of monoclonal antibodies to Na+,K+-ATPase. Methods In Enzymology 1988, 156: 392-413. PMID: 2835622, DOI: 10.1016/0076-6879(88)56039-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalEnzyme-Linked Immunosorbent AssayFluorescent Antibody TechniqueImmunohistochemistryIndicators and ReagentsKidney TubulesMiceSodium-Potassium-Exchanging ATPase
1986
Mesangial cell hillocks. Nodular foci of exaggerated growth of cells and matrix in prolonged culture.
Sterzel R, Lovett D, Foellmer H, Perfetto M, Biemesderfer D, Kashgarian M. Mesangial cell hillocks. Nodular foci of exaggerated growth of cells and matrix in prolonged culture. American Journal Of Pathology 1986, 125: 130-40. PMID: 3535527, PMCID: PMC1888434.Peer-Reviewed Original ResearchConceptsMesangial cellsNecrotic cell debrisCollagen type IMesangial matrix productionCultured mesangial cellsGlomerular mesangial cellsIsolated rat glomeruliType ISpecific rabbit polyclonal antibodiesNodular fociFetal calf serumDiseased glomeruliMC proliferationRabbit polyclonal antibodyStellate cellsNormal glomeruliMatrix proteinsInterstitial collagen types ICell debrisImmunocytochemical studyNodular accumulationMetabolic stimuliIntracellular fibrilsRat glomeruliGlomeruliNo aggravation of the course of experimental glomerulonephritis in spontaneously hypertensive rats.
Stein H, Sterzel R, Hunt J, Pabst R, Kashgarian M. No aggravation of the course of experimental glomerulonephritis in spontaneously hypertensive rats. American Journal Of Pathology 1986, 122: 520-30. PMID: 3513602, PMCID: PMC1888211.Peer-Reviewed Original ResearchMeSH KeywordsAlbuminuriaAnimalsAntibodies, Anti-IdiotypicAntibody FormationAutoradiographyBlood PressureFluorescent Antibody TechniqueGlomerulonephritisHistocytochemistryHypertensionImmune System DiseasesImmunoglobulin GKidneyMaleMicroscopy, ElectronRabbitsRatsRats, Inbred SHRRats, Inbred WKYThymidineConceptsWistar-Kyoto controlsBlood pressureGBM nephritisRenal injurySystemic hypertensionHypertensive ratsNormotensive Wistar-Kyoto (WKY) controlsAntiglomerular basement membrane nephritisUrinary albumin excretionSystolic blood pressureIgG antibody productionHumoral antibody responseNonspecific esteraseLight microscopyAlbumin excretionAutoregulatory capacityControl SHRMild hypertensionMorphologic injuryCreatinine clearanceGlomerular alterationsSerum creatinineNSE stainingHumoral responseAntibody responseProgressive Glomerular Sclerosis in Experimental Antiglomerular Basement Membrane Glomerulonephritis
Foellmer H, Sterzel R, Kashgarian M. Progressive Glomerular Sclerosis in Experimental Antiglomerular Basement Membrane Glomerulonephritis. American Journal Of Kidney Diseases 1986, 7: 5-11. PMID: 3510536, DOI: 10.1016/s0272-6386(86)80050-6.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateAntiglomerular basement membrane glomerulonephritisProgressive glomerular sclerosisGlomerular sclerosisPara-aminohippuric acidGlomerular hypercellularityFiltration fractionWistar ratsInitial inflammatory changesNormal renal clearanceIntrinsic glomerular cellsChronic glomerulosclerosisRenal insufficiencyInflammatory changesAcute phaseChronic phaseSignificant proteinuriaFiltration rateLeukocytic infiltrationCrescent formationFunctional abnormalitiesSclerotic processRenal clearanceGlomerular cellsGlomerulonephritis
1985
Monoclonal antibody to Na,K-ATPase: Immunocytochemical localization along nephron segments
Kashgarian M, Biemesderfer D, Caplan M, Forbush B. Monoclonal antibody to Na,K-ATPase: Immunocytochemical localization along nephron segments. Kidney International 1985, 28: 899-913. PMID: 3003443, DOI: 10.1038/ki.1985.216.Peer-Reviewed Original ResearchConceptsMonoclonal antibodiesNephron segmentsEvidence of labellingRenal tubular epithelial cellsTubular epithelial cellsK-ATPaseThick ascending limbOnly principal cellsDifferent nephron segmentsDog antigensBasal-lateral membranesSignificant antibodiesAscending limbMesangial regionHenle's loopOccasional cellsRenal medullaOuter renal medullaAbundant antibodyAntibodiesApical labelingTubule segmentsPrincipal cellsEpithelial cellsImmunocytochemical localization
1983
Role of angiotensin II-induced renal functional changes in mesangial deposition of exogenous ferritin in rats.
Stein H, Feddergreen W, Kashgarian M, Sterzel R. Role of angiotensin II-induced renal functional changes in mesangial deposition of exogenous ferritin in rats. Laboratory Investigation 1983, 49: 270-80. PMID: 6350702.Peer-Reviewed Original ResearchMeSH KeywordsAlbuminuriaAngiotensin IIAnimalsBlood PressureFerritinsFluorescent Antibody TechniqueInulinKidneyKidney GlomerulusKineticsMacromolecular SubstancesMaleP-Aminohippuric AcidRatsRats, Inbred StrainsConceptsBlood pressureFiltration fractionMesangial localizationAngiotensin IIFunctional changesAcute angiotensin IIAminohippuric acid clearanceRenal functional changesSystolic blood pressureExcretion of albuminSingle intravenous doseGlomerular capillary permeabilityGroups of ratsInjection of ferritinPressor doseAcid clearanceIntravenous doseMesangial depositionMesangial accumulationFerritin concentrationInulin clearanceTreatment periodRenal tissueSerial measurementsFerritin depositionDisposal of ferritin in the glomerular mesangium of rats
Sterzel R, Perfetto M, Biemesderfer D, Kashgarian M. Disposal of ferritin in the glomerular mesangium of rats. Kidney International 1983, 23: 480-488. PMID: 6188874, DOI: 10.1038/ki.1983.45.Peer-Reviewed Original ResearchConceptsGlomerular mesangiumDay 3DE ratsVascular poleIa-negative cellsMacula densa cellsIa-positive cellsGlomerular stainingMacula densaMesangial patternIntravenous injectionRenal tissueFerritin injectionIa antigensGlomerular tuftBearing cellsMesangial matrixMesangial cellsLacis areaMesangiumRatsCryostat sectionsInterstitial areaStudy periodFerritin
1982
Mesangial disposal of glomerular immune deposits in acute malarial glomerulonephritis of rats.
Sterzel R, Ehrich J, Lucia H, Thomson D, Kashgarian M. Mesangial disposal of glomerular immune deposits in acute malarial glomerulonephritis of rats. Laboratory Investigation 1982, 46: 209-14. PMID: 7038293.Peer-Reviewed Original ResearchConceptsGlomerular immune depositsImmune depositsElectron-dense depositsDay 10Day 20Young Sprague-Dawley ratsRenal functional damageUrinary protein excretionInfiltration of monocytesGlomerular cell countSprague-Dawley ratsEsterase-positive monocytesGlomerular basement membraneAcute malariaProtein excretionGlomerular injuryMesangial distributionSerum urea valuesDay 100Granular depositionImmune complexesFunctional damageNormal rangeMesangial matrixCell count
1980
Activation of the Classic Complement Pathway in Patients with the C3 Nephritic Factor
McLean R, Siegel N, Kashgarian M. Activation of the Classic Complement Pathway in Patients with the C3 Nephritic Factor. Nephron 1980, 25: 57-64. PMID: 6986570, DOI: 10.1159/000181754.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultBasement MembraneBiopsyCapillariesChildComplement ActivationComplement C3 Nephritic FactorComplement Inactivator ProteinsComplement Pathway, ClassicalFemaleFluorescent Antibody TechniqueGlomerulonephritisHemolysisHumansImmunoelectrophoresis, Two-DimensionalKidneyLipodystrophyMagnesiumMaleMicroscopy, ElectronConceptsC3 nephritic factorNephritic factorPartial lipodystrophyClassic complement pathwayDense deposit diseaseDense intramembranous depositsRenal diseaseDeposit diseaseIntramembranous depositsPatientsFactor assaysComplement pathwayComplement componentsUltrastructural evidenceCurrent criteriaLipodystrophyDiseaseType IHemolytic assayMCGNAssays
1975
Diffuse glomerulonephritis in rabbits with Streptococcus viridans endocarditis.
Arnold S, Valone J, Askenase P, Kashgarian M, Freedman L. Diffuse glomerulonephritis in rabbits with Streptococcus viridans endocarditis. Laboratory Investigation 1975, 32: 681-9. PMID: 125360.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialComplement C3Disease Models, AnimalEndocarditis, BacterialFluorescent Antibody TechniqueGlomerulonephritisHistological TechniquesImmune Complex DiseasesImmunizationImmunoglobulin GKidneyMaleMicroscopy, ElectronMicroscopy, FluorescenceRabbitsRheumatoid FactorSplenomegalyStreptococcal InfectionsConceptsInfective endocarditisDiffuse glomerulonephritisSubendothelial electron-dense depositsStreptococcus viridans endocarditisDeposition of IgGDiffuse proliferative glomerulonephritisSerum complement levelsAcute diffuse proliferative glomerulonephritisPositive blood culturesElectron microscopic findingsElectron-dense depositsComplement levelsGlomerular changesValvular vegetationsRheumatoid factorLeukocytic infiltrationMesangial proliferationProliferative glomerulonephritisBlood culturesImmune mechanismsCapillary occlusionMorphologic findingsIntravenous administrationMicroscopic findingsEndocarditis