2001
Differential Activation of the STAT Pathway by Angiotensin II via Angiotensin Type 1 and Type 2 Receptors in Cultured Human Fetal Mesangial Cells
Seebach F, Welte T, Fu X, Block L, Kashgarian M. Differential Activation of the STAT Pathway by Angiotensin II via Angiotensin Type 1 and Type 2 Receptors in Cultured Human Fetal Mesangial Cells. Experimental And Molecular Pathology 2001, 70: 265-273. PMID: 11418005, DOI: 10.1006/exmp.2001.2361.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IICells, CulturedDNA-Binding ProteinsFetusGlomerular MesangiumHumansKineticsMilk ProteinsPhosphorylationProtein TransportReceptor, Angiotensin, Type 1Receptor, Angiotensin, Type 2Receptors, AngiotensinSTAT1 Transcription FactorSTAT2 Transcription FactorSTAT5 Transcription FactorTrans-ActivatorsConceptsDNA-binding activityHuman mesangial cellsStat5 DNA-binding activityNuclear translocationSTAT-DNA bindingAngiotensin IISTAT5 tyrosine phosphorylationElectromobility shift assaysReceptor subtype-specific mannerMesangial cellsReceptor-specific signalingSTAT proteinsTyrosine phosphorylationShift assaysTranscription pathwayHuman fetal mesangial cellsSignal transducerSubtype-specific mannerCellular responsesSTAT pathwayAngiotensin receptor type 2Renal mesangial cellsAngiotensin II resultsVasoactive peptide angiotensin IIPossible regulatorMolecular mechanisms of TGF‐β antagonism by interferon γ and cyclosporine A in lung fibroblasts
EICKELBERG O, PANSKY A, KOEHLER E, BIHL M, TAMM M, HILDEBRAND P, PERRUCHOUD A, KASHGARIAN M, ROTH M. Molecular mechanisms of TGF‐β antagonism by interferon γ and cyclosporine A in lung fibroblasts. The FASEB Journal 2001, 15: 797-806. PMID: 11259398, DOI: 10.1096/fj.00-0233com.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedCollagenCyclosporineDNA-Binding ProteinsExtracellular MatrixFibroblastsGenes, ReporterHumansInterferon-gammaLungModels, BiologicalOligonucleotides, AntisenseProto-Oncogene Proteins c-junPulmonary FibrosisSignal TransductionSTAT1 Transcription FactorTrans-ActivatorsTranscription Factor AP-1Transforming Growth Factor betaConceptsCollagen depositionLung fibroblastsIFN-gammaExcess extracellular matrix depositionGrowth factor-beta activityPrimary human lung fibroblastsFibrotic lung diseaseHuman lung fibroblastsTGF-beta antagonismAP-1IFN-gamma treatmentExtracellular matrix depositionLung fibrosisFatal conditionLung diseaseMolecular mechanismsPathological featuresInterferon γTranscription factor AP-1Future therapiesPharmacological agentsJunD homodimersFactor AP-1Novel targetDirect inhibition
2000
In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse
Schechner J, Nath A, Zheng L, Kluger M, Hughes C, Sierra-Honigmann M, Lorber M, Tellides G, Kashgarian M, Bothwell A, Pober J. In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 9191-9196. PMID: 10890921, PMCID: PMC16844, DOI: 10.1073/pnas.150242297.Peer-Reviewed Original Research
1998
Antibodies against mesangial cells and their secretory products in chronic renal allograft rejection in the rat.
Paul L, Muralidharan J, Muzaffar S, Manting E, Valentin J, de Heer E, Kashgarian M. Antibodies against mesangial cells and their secretory products in chronic renal allograft rejection in the rat. American Journal Of Pathology 1998, 152: 1209-23. PMID: 9588890, PMCID: PMC1858589.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoantibodiesAutoantigensBasement MembraneBiglycanBlotting, WesternCells, CulturedChronic DiseaseDecorinEndothelium, VascularExtracellular Matrix ProteinsFlow CytometryFluorescent Antibody Technique, IndirectGlomerular MesangiumGraft RejectionKidney TransplantationMaleProteoglycansRatsRats, Inbred F344Rats, Inbred LewTransplantation, HomologousConceptsMesangial cell culture supernatantsChronic renal allograft rejectionRenal allograft rejectionMesangial cellsChronic rejectionAllograft rejectionCell culture supernatantsChronic renal transplant rejectionFlow cytometryWestern blotEndothelial cellsGlomerular basement membrane antigenPost-transplant seraRenal transplant rejectionCell-mediated immunityPost-immunization seraPresence of antibodiesMesangial cell surfaceStrong autoantibody responseCulture supernatantsCultured mesangial cellsIndirect immunofluorescent studiesBasement membrane antigensIndirect immunofluorescent stainingTissue repair process
1996
Na-K-ATPase alpha-isoform expression in heart and vascular endothelia: cellular and developmental regulation
Zahler R, Sun W, Ardito T, Kashgarian M. Na-K-ATPase alpha-isoform expression in heart and vascular endothelia: cellular and developmental regulation. American Journal Of Physiology 1996, 270: c361-c371. PMID: 8772464, DOI: 10.1152/ajpcell.1996.270.1.c361.Peer-Reviewed Original ResearchConceptsCardiac conduction systemAlpha 2Alpha 1Alpha 3Vascular endotheliumConduction systemHigh ouabain affinityNa-K-ATPase isoformsDevelopmental regulationAlpha 3 expressionImportant species differencesNeonatal rat myocardiumIsoform patternNeonatal ratsPapillary musclesDistinct physiological rolesSmooth muscleImmunohistochemical analysisConduction tissueVentricular myocardiumOuabain affinityRat myocardiumNormal endotheliumRat heartT-tubule system
1995
PDGF and TGF-β mediate collagen production by mesangial cells exposed to advanced glycosylation end products
Throckmorton D, Brogden A, Min B, Rasmussen H, Kashgarian M. PDGF and TGF-β mediate collagen production by mesangial cells exposed to advanced glycosylation end products. Kidney International 1995, 48: 111-117. PMID: 7564067, DOI: 10.1038/ki.1995.274.Peer-Reviewed Original Research
1994
A granulocyte inhibitory protein overexpressed in chronic renal disease regulates expression of interleukin 6 and interleukin 8.
Ziesche R, Roth M, Papakonstantinou E, Nauck M, Hörl W, Kashgarian M, Block L. A granulocyte inhibitory protein overexpressed in chronic renal disease regulates expression of interleukin 6 and interleukin 8. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 301-305. PMID: 8278382, PMCID: PMC42935, DOI: 10.1073/pnas.91.1.301.Peer-Reviewed Original ResearchConceptsGranulocyte inhibitory proteinsInterleukin-6Chronic renal diseaseChronic renal failureProgression of glomerulosclerosisHuman mesangial cellsInhibitory proteinPluripotential effectsRenal failureRenal diseaseCytokine expressionIL-6IL-8Interleukin-8Inflammatory reactionGIP resultsGlomerular responseMesangial cellsC-jun mRNAC-fos geneC-JunDe novo synthesisNovo synthesisExpressionOverall effectThe Contribution of Mesangial Cell Collagen Synthesis to the Pathogenesis of Diabetic Nephropathy1
Kashgarian M, Oshima S, Takeuchi A, Throckmorton D, Rasmussen H. The Contribution of Mesangial Cell Collagen Synthesis to the Pathogenesis of Diabetic Nephropathy1. Contributions To Nephrology 1994, 107: 132-139. PMID: 8004959, DOI: 10.1159/000422971.Peer-Reviewed Original ResearchTransforming growth factor-beta 1 stimulates glomerular mesangial cell synthesis of the 72-kd type IV collagenase.
Marti H, Lee L, Kashgarian M, Lovett D. Transforming growth factor-beta 1 stimulates glomerular mesangial cell synthesis of the 72-kd type IV collagenase. American Journal Of Pathology 1994, 144: 82-94. PMID: 8291614, PMCID: PMC1887110.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, NorthernCells, CulturedCollagenasesEnzyme-Linked Immunosorbent AssayGlomerular MesangiumHumansImmunohistochemistryMatrix Metalloproteinase 9Molecular ProbesMolecular Sequence DataNephritisRatsRats, WistarTranscription, GeneticTransforming Growth Factor betaConceptsType IV collagenaseHuman mesangial cellsType IVMesangial cellsImmune complex-mediated glomerulonephritisMRNA expressionType IV collagenase mRNA expressionCultured human mesangial cellsCollagenase mRNA expressionMesangial cell synthesisGlomerular inflammatory processesTIMP-1 synthesisNephritic ratsInflammatory processGlomerular matrixMesangial distributionMatrix protein synthesisTIMP-1Direct stimulationTissue inhibitorNormal animalsMesangial compartmentMatrix metalloproteinase synthesisStimulatory effectTIMP-1 inhibitor
1993
Spatial organization of the extracellular matrix modulates the expression of PDGF-receptor subunits in mesangial cells
Marx M, Daniel T, Kashgarian M, Madri J. Spatial organization of the extracellular matrix modulates the expression of PDGF-receptor subunits in mesangial cells. Kidney International 1993, 43: 1027-1041. PMID: 8510381, DOI: 10.1038/ki.1993.145.Peer-Reviewed Original ResearchConceptsPDGF receptor subunitsMesangial cell phenotypeCell surfaceExtracellular matrixCell phenotypeExtracellular matrix environmentSpatial organizationPDGF responsivenessPDGF receptor alphaTwo-dimensional cultureMulticellular structuresThree-dimensional cultureMetabolic labelingDifferential expressionQuiescent cellsRat mesangial cell growthCell growthPDGF isoforms AASubunitsMatrix environmentCell proliferationPDGF isoformsReceptor subunitsUltrastructural analysisSurface expressionEffect of transforming growth factor-β on extracellular matrix productionby cultured rat mesangial cells
ISHIMURA E, MORII H, STERZEL R, KASHGARIAN M. Effect of transforming growth factor-β on extracellular matrix productionby cultured rat mesangial cells. 日本腎臓学会誌 1993, 35: 311-320. PMID: 8341006, DOI: 10.14842/jpnjnephrol1959.35.311.Peer-Reviewed Original ResearchConceptsNorthern blottingCultured rat mesangial cellsRat mesangial cellsCollagen IExtracellular matrix constituentsMesangial cellsGrowth factorEnzyme-linked immunoadsorbent assayGene expressionProtein productionSingle growth factorECM constituentsProtein synthesisGenetic expressionMatrix componentsLamininExpressionGlomerular remodelingECM accumulationTGF-beta administrationBlottingMRNA expressionMatrix constituentsCellsGlomerular injury
1989
Formation of extracellular matrix by cultured rat mesangial cells.
Ishimura E, Sterzel R, Budde K, Kashgarian M. Formation of extracellular matrix by cultured rat mesangial cells. American Journal Of Pathology 1989, 134: 843-55. PMID: 2650558, PMCID: PMC1879799.Peer-Reviewed Original Research
1986
Mesangial cell hillocks. Nodular foci of exaggerated growth of cells and matrix in prolonged culture.
Sterzel R, Lovett D, Foellmer H, Perfetto M, Biemesderfer D, Kashgarian M. Mesangial cell hillocks. Nodular foci of exaggerated growth of cells and matrix in prolonged culture. American Journal Of Pathology 1986, 125: 130-40. PMID: 3535527, PMCID: PMC1888434.Peer-Reviewed Original ResearchConceptsMesangial cellsNecrotic cell debrisCollagen type IMesangial matrix productionCultured mesangial cellsGlomerular mesangial cellsIsolated rat glomeruliType ISpecific rabbit polyclonal antibodiesNodular fociFetal calf serumDiseased glomeruliMC proliferationRabbit polyclonal antibodyStellate cellsNormal glomeruliMatrix proteinsInterstitial collagen types ICell debrisImmunocytochemical studyNodular accumulationMetabolic stimuliIntracellular fibrilsRat glomeruliGlomeruli