2022
Urine Uromodulin as a Biomarker of Kidney Tubulointerstitial Fibrosis
Melchinger H, Calderon-Gutierrez F, Obeid W, Xu L, Shaw MM, Luciano RL, Kuperman M, Moeckel GW, Kashgarian M, Wilson FP, Parikh CR, Moledina DG. Urine Uromodulin as a Biomarker of Kidney Tubulointerstitial Fibrosis. Clinical Journal Of The American Society Of Nephrology 2022, 17: 1284-1292. PMID: 35948365, PMCID: PMC9625093, DOI: 10.2215/cjn.04360422.Peer-Reviewed Original ResearchConceptsInterstitial fibrosis/tubular atrophyUrine uromodulinTubular atrophyThick ascending limbUrine albuminSerum creatinineKidney biopsyTubulointerstitial fibrosisMultivariable linear regression modelsTime of biopsyKidney's thick ascending limbAcademic medical centerHuman kidney biopsiesKidney tubulointerstitial fibrosisTubular healthMultivariable analysisHistologic featuresHistologic findingsHistologic changesKidney fibrosisIndependent associationFibrotic modelMultivariable modelMedical CenterMurine model
2021
DNA glycosylase deficiency leads to decreased severity of lupus in the Polb-Y265C mouse model
Paluri SL, Burak M, Senejani AG, Levinson M, Rahim T, Clairmont K, Kashgarian M, Alvarado-Cruz I, Meas R, Cardó-Vila M, Zeiss C, Maher S, Bothwell ALM, Coskun E, Kant M, Jaruga P, Dizdaroglu M, Lloyd R, Sweasy JB. DNA glycosylase deficiency leads to decreased severity of lupus in the Polb-Y265C mouse model. DNA Repair 2021, 105: 103152. PMID: 34186496, PMCID: PMC8635285, DOI: 10.1016/j.dnarep.2021.103152.Peer-Reviewed Original Research
2020
B cell-intrinsic TLR9 expression is protective in murine lupus
Tilstra JS, John S, Gordon RA, Leibler C, Kashgarian M, Bastacky S, Nickerson KM, Shlomchik MJ. B cell-intrinsic TLR9 expression is protective in murine lupus. Journal Of Clinical Investigation 2020, 130: 3172-3187. PMID: 32191633, PMCID: PMC7260024, DOI: 10.1172/jci132328.Peer-Reviewed Original ResearchConceptsToll-like receptor 9Systemic lupus erythematosusTLR9 deficiencyTLR9 expressionDendritic cellsB cellsPlasmacytoid dendritic cellsAnti-nucleosome antibodiesLupus erythematosusAutoantibody productionMurine lupusSLE pathogenesisTLR9 signalsReceptor 9Cell type-specific effectsType-specific effectsDisease pathogenesisTherapeutic potentialSelective deletionDiseaseNonredundant roleNephritisLupusPathogenesisDeficiency
2011
A Small-Molecule Macrophage Migration Inhibitory Factor Antagonist Protects against Glomerulonephritis in Lupus-Prone NZB/NZW F1 and MRL/lpr Mice
Leng L, Chen L, Fan J, Greven D, Arjona A, Du X, Austin D, Kashgarian M, Yin Z, Huang XR, Lan HY, Lolis E, Nikolic-Paterson D, Bucala R. A Small-Molecule Macrophage Migration Inhibitory Factor Antagonist Protects against Glomerulonephritis in Lupus-Prone NZB/NZW F1 and MRL/lpr Mice. The Journal Of Immunology 2011, 186: 527-538. PMID: 21106847, PMCID: PMC3124407, DOI: 10.4049/jimmunol.1001767.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell Migration InhibitionFemaleGene Expression ProfilingGenetic Predisposition to DiseaseGlomerulonephritisHumansIntramolecular OxidoreductasesIsoxazolesLupus Erythematosus, SystemicMacrophage Migration-Inhibitory FactorsMiceMice, Inbred MRL lprMice, Inbred NZBMice, KnockoutMolecular Sequence DataRandom AllocationReceptors, ImmunologicConceptsMacrophage migration inhibitory factorMRL/lpr miceNZB/NZW F1Systemic lupus erythematosusMigration inhibitory factorLupus erythematosusLpr miceDihydro-5-isoxazole acetic acid methyl esterHigh-expression MIF allelesInhibitory factorExcessive proinflammatory responsesRetention of monocytesEnd-organ injuryEnd-organ damageProinflammatory cytokine productionDifferent autoimmune diseasesB cell activationMIF antagonismMIF antagonistAutoantibody productionChemokine expressionProinflammatory cytokinesAutoimmune diseasesCytokine productionMIF alleles
2010
Dendritic Cells in Lupus Are Not Required for Activation of T and B Cells but Promote Their Expansion, Resulting in Tissue Damage
Teichmann LL, Ols ML, Kashgarian M, Reizis B, Kaplan DH, Shlomchik MJ. Dendritic Cells in Lupus Are Not Required for Activation of T and B Cells but Promote Their Expansion, Resulting in Tissue Damage. Immunity 2010, 33: 967-978. PMID: 21167752, PMCID: PMC3010763, DOI: 10.1016/j.immuni.2010.11.025.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoantibodiesBlood Component RemovalB-LymphocytesCell DifferentiationCell MovementDendritic CellsDisease Models, AnimalHumansImmunoglobulin Class SwitchingInterferon-gammaLupus Erythematosus, SystemicLymphocyte ActivationMiceMice, Inbred C57BLMice, Inbred MRL lprT-Lymphocyte SubsetsT-Lymphocytes, RegulatoryConceptsDendritic cellsAdaptive immune responsesMurine lupusImmune responseAbsence of DCsEffects of DCsSystemic lupus erythematosusSerum immunoglobulin concentrationsPotential therapeutic targetLupus erythematosusInterstitial infiltratesAutoimmune disordersHumoral responseAutoantibody concentrationsPlasmablast numbersImmunoglobulin concentrationsTherapeutic targetB cellsTissue damageLupusCell numberCellsInitial activationComplex roleActivationAltered renal proximal tubular endocytosis and histology in mice lacking myosin‐VI
Gotoh N, Yan Q, Du Z, Biemesderfer D, Kashgarian M, Mooseker MS, Wang T. Altered renal proximal tubular endocytosis and histology in mice lacking myosin‐VI. Cytoskeleton 2010, 67: 178-192. PMID: 20175219, PMCID: PMC3468331, DOI: 10.1002/cm.20435.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdaptor Proteins, Signal TransducingAdaptor Proteins, Vesicular TransportAnimalsApoptosis Regulatory ProteinsCell TransdifferentiationEndocytosisHorseradish PeroxidaseKidney Tubules, ProximalLow Density Lipoprotein Receptor-Related Protein-2MiceMice, Neurologic MutantsMicrofilament ProteinsMicrovilliMyosin Heavy ChainsVesicular Transport ProteinsConceptsProximal tubule cellsAlbumin excretionSv miceTubule cellsProximal tubule brush borderGlomerular flow rateProximal tubular dilationUrinary albumin excretionElevated blood pressureReduced body weightProximal tubular endocytosisRenal proximal tubule brush borderTubule brush borderProximal tubular uptakeBlood acid-base parametersMouse kidney proximal tubulesKidney proximal tubulesBlood pressureUrine volumeUrinary excretionAcid-base parametersRenal homogenatesTubular dilationPlasma concentrationsRenal clearance
2008
Disruption of Myosin 1e Promotes Podocyte Injury
Krendel M, Kim SV, Willinger T, Wang T, Kashgarian M, Flavell RA, Mooseker MS. Disruption of Myosin 1e Promotes Podocyte Injury. Journal Of The American Society Of Nephrology 2008, 20: 86-94. PMID: 19005011, PMCID: PMC2615733, DOI: 10.1681/asn.2007111172.Peer-Reviewed Original ResearchConceptsChronic renal injuryNormal glomerular filtrationGlomerular basement membraneRenal injuryKidney inflammationPodocyte foot processesGlomerular diseasePodocyte injuryGlomerular filtrationRenal tissueFoot processesPodocyte functionBasement membraneInjuryUltrastructural levelDiseaseMiceType IProteinuriaInflammationKidneyImportant rolePodocytes
2000
In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse
Schechner J, Nath A, Zheng L, Kluger M, Hughes C, Sierra-Honigmann M, Lorber M, Tellides G, Kashgarian M, Bothwell A, Pober J. In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 9191-9196. PMID: 10890921, PMCID: PMC16844, DOI: 10.1073/pnas.150242297.Peer-Reviewed Original Research
1997
A Tyrosine-Based Signal Targets H/K-ATPase to a Regulated Compartment and Is Required for the Cessation of Gastric Acid Secretion
Courtois-Coutry N, Roush D, Rajendran V, McCarthy J, Geibel J, Kashgarian M, Caplan M. A Tyrosine-Based Signal Targets H/K-ATPase to a Regulated Compartment and Is Required for the Cessation of Gastric Acid Secretion. Cell 1997, 90: 501-510. PMID: 9267030, DOI: 10.1016/s0092-8674(00)80510-3.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCOS CellsCytomegalovirusDNA PrimersEndocytosisGastric AcidGastric MucosaH(+)-K(+)-Exchanging ATPaseMacromolecular SubstancesMiceMice, TransgenicMicroscopy, ImmunoelectronMutagenesis, Site-DirectedParietal Cells, GastricPolymerase Chain ReactionPromoter Regions, GeneticRecombinant ProteinsSignal TransductionTransfectionTyrosineConceptsK-ATPase beta subunitTyrosine-based signalsK-ATPaseTyrosine-based endocytosis signalTyrosine residuesBeta subunitIntracellular storage compartmentEndocytosis signalCytoplasmic tailMutant betaRegulated compartmentsSecrete acidResidue sequenceStorage compartmentCell surfaceCell plasmalemmaSubunitsTransgenic miceParietal cellsGastric glandsCompartmentsSecretionAcid secretionReinternalizationPlasmalemma
1988
[36] Preparation and use of monoclonal antibodies to Na+,K+-ATPase
Kashgarian M, Biemesderfer D. [36] Preparation and use of monoclonal antibodies to Na+,K+-ATPase. Methods In Enzymology 1988, 156: 392-413. PMID: 2835622, DOI: 10.1016/0076-6879(88)56039-1.Peer-Reviewed Original Research
1976
Lupus nephritis: natural history and therapy.
Hecht B, Siegel N, Kashgarian M, Hayslett J. Lupus nephritis: natural history and therapy. Perspectives In Nephrology And Hypertension 1976, 3: 21-41. PMID: 175347.Peer-Reviewed Original Research