Lack of vimentin impairs endothelial differentiation of embryonic stem cells
Boraas L, Ahsan T. Lack of vimentin impairs endothelial differentiation of embryonic stem cells. Scientific Reports 2016, 6: 30814. PMID: 27480130, PMCID: PMC4969593, DOI: 10.1038/srep30814.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsEmbryoid bodiesKnockout embryonic stem cellsWild-type embryonic stem cellsStem cellsEndothelial phenotypeCell typesWT EBsSpecification pathwayCytoskeletal filament vimentinGene expressionSpontaneous differentiationPluripotency markersEndothelial differentiationProper functionVE-cadherinPresence of vimentinPhenotypeProtein expressionDifferentiationDecreased expressionExpressionEndothelial cellsCellsAdult miceCytoskeletal Expression and Remodeling in Pluripotent Stem Cells
Boraas L, Guidry J, Pineda E, Ahsan T. Cytoskeletal Expression and Remodeling in Pluripotent Stem Cells. PLOS ONE 2016, 11: e0145084. PMID: 26771179, PMCID: PMC4714815, DOI: 10.1371/journal.pone.0145084.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsInduced pluripotent stem cellsPluripotent stem cellsCytoskeletal proteinsStem cellsMouse embryonic stem cellsEmbryoid body modelIPSC samplesUndifferentiated pluripotent stem cellsSimilar gene expressionCell-based therapiesLamin ACytoskeletal networkEmbryonic fibroblastsCytoskeletal intermediate filamentsGene expressionParental phenotypesGene levelSmooth muscle cell actinSpontaneous differentiationCytoskeletal markersCell actinDifferentiation studiesCytoskeletal expressionIntermediate filaments