2024
Transcription factor-dependent regulatory networks of sexual reproduction in Fusarium graminearum.
Kim W, Kim D, Wang Z, Liu M, Townsend J, Trail F. Transcription factor-dependent regulatory networks of sexual reproduction in Fusarium graminearum. MBio 2024, e0303024. PMID: 39589130, DOI: 10.1128/mbio.03030-24.Peer-Reviewed Original ResearchMating-type locusSexual reproductionKnockout mutantsTranscription factorsTranscriptome dataStage of sexual developmentRegulatory networksTranscriptional cascadeDevelopmental phenotypesSexual developmentAscospore productionRegulation of sexual developmentUpstream activatorSexual fruiting bodiesTranscriptional regulatory cascadeMulticellular body planRegulate sexual developmentMultiple transcription factorsFusarium graminearum</i>Systems biology approachComplex reproductive biologyEarly stages of sexual developmentArrest phenotypeTF networksFilamentous fungi
2017
The ancestral levels of transcription and the evolution of sexual phenotypes in filamentous fungi
Trail F, Wang Z, Stefanko K, Cubba C, Townsend JP. The ancestral levels of transcription and the evolution of sexual phenotypes in filamentous fungi. PLOS Genetics 2017, 13: e1006867. PMID: 28704372, PMCID: PMC5509106, DOI: 10.1371/journal.pgen.1006867.Peer-Reviewed Original ResearchConceptsGene expressionFilamentous fungiCommon garden environmentBody developmentWide gene expressionGene expression phenotypesGene deletion studiesGene expression measurementsMulticellular developmentDivergent speciesSexual phenotypeGene familyEvolved increasesDivergent morphologyKnockout phenotypesAncestral levelsWhole genomeGarden environmentExpression phenotypesDeletion studiesRelevant genesPhenotypic analysisDevelopmental stagesExpression measurementsBody morphology
2007
Enabling a Community to Dissect an Organism: Overview of the Neurospora Functional Genomics Project
Dunlap JC, Borkovich KA, Henn MR, Turner GE, Sachs MS, Glass NL, McCluskey K, Plamann M, Galagan JE, Birren BW, Weiss RL, Townsend JP, Loros JJ, Nelson MA, Lambreghts R, Colot HV, Park G, Collopy P, Ringelberg C, Crew C, Litvinkova L, DeCaprio D, Hood HM, Curilla S, Shi M, Crawford M, Koerhsen M, Montgomery P, Larson L, Pearson M, Kasuga T, Tian C, Baştürkmen M, Altamirano L, Xu J. Enabling a Community to Dissect an Organism: Overview of the Neurospora Functional Genomics Project. Advances In Genetics 2007, 57: 49-96. PMID: 17352902, PMCID: PMC3673015, DOI: 10.1016/s0065-2660(06)57002-6.Peer-Reviewed Original ResearchConceptsFunctional genomics projectsGenomics projectsFilamentous fungiFilamentous fungus NeurosporaFunctional genomic analysisNon-yeast fungiFunctional genomicsNeurospora genomeFungus NeurosporaNovel genesPositional cloningNeurospora crassaAntisense transcriptsGenomic analysisSNP mapAlternative promotersCDNA libraryExpression analysisGene replacementMutant strainSystematic disruptionExpression dataPhenotypic analysisNeurosporaConditions of growth
2005
Long-oligomer microarray profiling in Neurospora crassa reveals the transcriptional program underlying biochemical and physiological events of conidial germination
Kasuga T, Townsend JP, Tian C, Gilbert LB, Mannhaupt G, Taylor JW, Glass NL. Long-oligomer microarray profiling in Neurospora crassa reveals the transcriptional program underlying biochemical and physiological events of conidial germination. Nucleic Acids Research 2005, 33: 6469-6485. PMID: 16287898, PMCID: PMC1283539, DOI: 10.1093/nar/gki953.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBayes TheoremBlotting, NorthernCluster AnalysisDatabases, Nucleic AcidDictyosteliumExpressed Sequence TagsGene Expression ProfilingGenes, FungalNeurospora crassaOligonucleotide Array Sequence AnalysisOligonucleotide ProbesPromoter Regions, GeneticReproducibility of ResultsRNA, MessengerSpores, FungalTranscription, GeneticUstilagoConceptsConidial germinationGene expression levelsNeurospora crassaGene expressionPhytopathogenic fungus Ustilago maydisSocial amoeba Dictyostelium discoideumFilamentous ascomycete speciesPutative regulatory componentFungus Ustilago maydisAmoeba Dictyostelium discoideumExpression levelsAscomycete speciesRibosomal biogenesisRelative gene expression levelsNovel genesTranscriptional programsUstilago maydisTranscriptional profilingDictyostelium discoideumFunctional predictionTranscriptional mechanismsRegulatory componentsFilamentous fungiExpression profilesMicroarray profiling