2023
Prior immunization against an intracellular antigen enhances subsequent red blood cell alloimmunization in mice
Jajosky R, Patel S, Wu S, Patel K, Covington M, Vallecillo-Zúniga M, Ayona D, Bennett A, Luckey C, Hudson K, Hendrickson J, Eisenbarth S, Josephson C, Zerra P, Stowell S, Arthur C. Prior immunization against an intracellular antigen enhances subsequent red blood cell alloimmunization in mice. Blood 2023, 141: 2642-2653. PMID: 36638335, PMCID: PMC10356576, DOI: 10.1182/blood.2022016588.Peer-Reviewed Original ResearchConceptsCD4 T cell responsesT cell responsesIntracellular antigensB cellsImmune primingRed blood cell alloimmunizationBlood cell alloantigensRate of alloimmunizationAdditional alloantibodiesAlloimmunization rateRBC alloantigensSubsequent transfusionsSame alloantigensPrior immunizationTransfusion recipientsDonor RBCsMouse modelNumerous antigensRBC antigensAlloantigensAlloimmunizationAntigenTransfusionAlloantibodiesRBCs
2021
Complement Plays a Critical Role in Inflammation-Induced Immunoprophylaxis Failure in Mice
Escamilla-Rivera V, Santhanakrishnan M, Liu J, Gibb DR, Forsmo JE, Foxman EF, Eisenbarth SC, Luckey CJ, Zimring JC, Hudson KE, Stowell SR, Hendrickson JE. Complement Plays a Critical Role in Inflammation-Induced Immunoprophylaxis Failure in Mice. Frontiers In Immunology 2021, 12: 704072. PMID: 34249009, PMCID: PMC8270673, DOI: 10.3389/fimmu.2021.704072.Peer-Reviewed Original ResearchConceptsImmunoprophylaxis failureRed blood cellsRBC transfusionComplement receptorsHuman KEL glycoproteinB cell activation thresholdWild-type micePresence of complementMurine red blood cellsTwo-hit modelRecipient inflammationIgG alloantibodiesInflammatory monocytesAdaptive immunityType miceB cellsRecipient complementTranslational relevanceKey cellsTransfusionMiceBlood cellsImmunoprophylaxis efficacyBaseline stateActivation thresholdMarginal zone B cells mediate a CD4 T-cell–dependent extrafollicular antibody response following RBC transfusion in mice
Zerra PE, Patel SR, Jajosky RP, Arthur CM, McCoy JW, Allen JWL, Chonat S, Fasano RM, Roback JD, Josephson CD, Hendrickson J, Stowell SR. Marginal zone B cells mediate a CD4 T-cell–dependent extrafollicular antibody response following RBC transfusion in mice. Blood 2021, 138: 706-721. PMID: 33876205, PMCID: PMC8394907, DOI: 10.1182/blood.2020009376.Peer-Reviewed Original ResearchConceptsMarginal zone B cellsRBC transfusionMZ B cellsB cellsHOD RBCsAlloantibody formationAntibody responseAntibody formationAntigen-specific germinal center B cellsB cell-deficient recipientsCD4 T-cell depletionRed blood cell transfusionCD4 T cell activationRBC alloantibody formationBlood cell transfusionT-cell depletionCD4 T cellsProbability of complicationsExtrafollicular antibody responsesGerminal center B cellsFollicular B cellsT cell activationRBC alloimmunizationCell transfusionSubsequent transfusions
2018
1 Type I Interferon Is Necessary and Sufficient for Alloimmunization to Transfused KEL-Expressing RBCs in Mice
Gibb D, Liu J, Natarajan P, Santhanakrishnan M, Madrid D, Eisenbarth S, Zimring J, Iwasaki A, Hendrickson J. 1 Type I Interferon Is Necessary and Sufficient for Alloimmunization to Transfused KEL-Expressing RBCs in Mice. American Journal Of Clinical Pathology 2018, 149: s163-s163. DOI: 10.1093/ajcp/aqx149.370.Peer-Reviewed Original ResearchIFNα/βAlloimmune responseType I interferonKEL RBCsRBC alloimmunizationWT miceIFNAR1 expressionInflammatory stimuliB cellsI interferonChimeric miceRBC antigensNew transgenic mouse modelCertain inflammatory disordersHuman KEL glycoproteinRed blood cell antigensIFNα/β productionToll-like receptorsInterferon regulatory factor 3Transgenic mouse modelBlood cell antigensRegulatory factor 3Non-hematopoietic cellsIgG alloantibodiesTransfusion protocol
2015
DOCK8-Deficient Mice Reveal a Crucial Role for Dendritic Cell Activity in the Initiation of the Allogeneic Response to Transfused Red Blood Cells
Eisenbarth S, Hendrickson J, Calabro S, Gallman A. DOCK8-Deficient Mice Reveal a Crucial Role for Dendritic Cell Activity in the Initiation of the Allogeneic Response to Transfused Red Blood Cells. Blood 2015, 126: 658. DOI: 10.1182/blood.v126.23.658.658.Peer-Reviewed Original ResearchAllogeneic red blood cellsDOCK8-deficient miceRed blood cellsT cellsDendritic cellsT cell activationAllogeneic responseB cellsImmune signalsCell activationT cell receptor transgenic miceSplenic dendritic cell subsetsBlood cellsDendritic cell activityInduction of alloimmunityDendritic cell subsetsHen egg lysozymeT cell primingDendritic cell migrationInnate immune cellsReceptor transgenic miceInnate immune signalsT cell zonesAntigen presenting cellsB cell responses
2014
NLRP10-Deficient Mice Reveal a Crucial Role for Dendritic Cell Activity in the Initiation of the Allogeneic Response to Transfused Red Blood Cells
Eisenbarth S, Hendrickson J, Calabro S, Gallman A. NLRP10-Deficient Mice Reveal a Crucial Role for Dendritic Cell Activity in the Initiation of the Allogeneic Response to Transfused Red Blood Cells. Blood 2014, 124: 4113. DOI: 10.1182/blood.v124.21.4113.4113.Peer-Reviewed Original ResearchAllogeneic red blood cellsRed blood cellsDendritic cellsT cell activationT cellsBlood cellsIntegral membrane proteinsAllogeneic responseBeta-globin promoterB cellsImmune signalsTriple fusion proteinCell activationT cell receptor transgenic miceInnate immune cytokine responsesRounds of divisionDendritic cell activityInduction of alloimmunityHen egg lysozymeSplenic dendritic cellsT cell primingReceptor transgenic miceImmune cytokine responsesInnate immune signalsGlobin promoter
2013
Alterations In Specific Immune Cell Subsets In Children With Sickle Cell Disease
Nickel R, Hendrickson J, Horan J, Garrett A, Robertson J, Osunkwo I, Kean L. Alterations In Specific Immune Cell Subsets In Children With Sickle Cell Disease. Blood 2013, 122: 2208. DOI: 10.1182/blood.v122.21.2208.2208.Peer-Reviewed Original ResearchSickle cell diseaseWhite blood cellsTotal white blood cellsChronic transfusionImmune cell subsetsHU patientsImmune cell populationsSCD patientsT cellsCell subsetsHealthy controlsCell diseaseB cellsGraft rejectionNK cellsImmune systemBone marrow transplant rejectionT-cell subset analysisCell populationsRed blood cell alloimmunizationPediatric sickle cell diseaseSpecific immune cell subsetsDifferent immune cell populationsAbnormal immune activationB cell analysis
2012
Marginal Zone B Cells Mediate Alloantibody Formation to a Clinically Significant Human RBC Antigen in a Murine Model
Girard-Pierce K, Stowell S, Smith N, Henry K, Zimring J, Hendrickson J. Marginal Zone B Cells Mediate Alloantibody Formation to a Clinically Significant Human RBC Antigen in a Murine Model. Blood 2012, 120: 843. DOI: 10.1182/blood.v120.21.843.843.Peer-Reviewed Original ResearchMZ B cellsHemolytic transfusion reactionsTransfusion of RBCsC57BL/6 recipient miceRecipient miceAlloimmune responseB cellsRBC antigensRBC alloimmunizationAlloantibody responsesPost transfusionMouse modelRBC clearanceFlow cytometryNovel transgenic mouse modelBlood borne antigensConsequences of alloimmunizationControl recipient miceDays post transfusionHuman KEL glycoproteinRBC-specific expressionB-cell depletionRed blood cell antigensAntibody-mediated immunityMarginal zone B cells
2011
Central T Cell Tolerance and Peripheral B Cell Tolerance for An RBC Autoantigen Are Incomplete in Healthy Mice; Implications for AIHA Pathogenesis
Hudson K, Hendrickson J, Cadwell C, Iwakoshi N, Zimring J. Central T Cell Tolerance and Peripheral B Cell Tolerance for An RBC Autoantigen Are Incomplete in Healthy Mice; Implications for AIHA Pathogenesis. Blood 2011, 118: 693. DOI: 10.1182/blood.v118.21.693.693.Peer-Reviewed Original ResearchAutoimmune hemolytic anemiaT cell toleranceOVA/CFAB cell tolerancePathogenesis of AIHAReactive T cellsT cellsHOD antigenAdoptive transferPeripheral toleranceCell toleranceB cellsB6 miceHen egg lysozymeHOD RBCsReactive CD4Humoral toleranceF1 miceImmune responseAntigen expressionTransgenic miceWild-type B6 miceWild-type C57BL/6 micePeripheral B cell toleranceOT-II CD4
2008
A Novel Model of Autoimmunity to Self Red Blood Cell Antigens
Hendrickson J, Cadwell C, Hillyer C, Zimring J. A Novel Model of Autoimmunity to Self Red Blood Cell Antigens. Blood 2008, 112: 3461. DOI: 10.1182/blood.v112.11.3461.3461.Peer-Reviewed Original ResearchAutoimmune hemolytic anemiaB1 B cellsSelf-reactive B cellsReactive B cellsAutoreactive B cellsB cell toleranceB cellsControl micePositive miceB cell maturationLymph nodesBone marrowCell toleranceRBC antigensCell maturationRBC-specific expressionRed blood cell antigensSystemic immune activationHuman blood group antigensAntigen hen egg lysozymeLife-threatening illnessConventional B cellsBlood cell antigensSigns of illnessHemoglobin breakdown products
2006
Recipient Inflammation That Increases Alloimmunization Also Enhances Consumption of Transfused RBCs by Dendritic Cells.
Hendrickson J, Roback J, Hillyer C, Zimring J. Recipient Inflammation That Increases Alloimmunization Also Enhances Consumption of Transfused RBCs by Dendritic Cells. Blood 2006, 108: 23. DOI: 10.1182/blood.v108.11.23.23.Peer-Reviewed Original ResearchLiver dendritic cellsDendritic cellsT cellsRBC antigensRed blood cellsImmune responseSplenic macrophagesB cellsRBC consumptionCo-stimulatory molecule expressionBlood cellsRed blood cell antigensMajority of patientsC57BL/6 recipient micePresence of inflammationAbsence of inflammationRole of antigenLymph node macrophagesBlood cell antigensRBC alloimmunizationRecipient inflammationAlloantibody responsesLymph nodesRBC transfusionPotent APCs