2020
Implantation of the clinical‐grade human neural stem cell line, CTX0E03, rescues the behavioral and pathological deficits in the quinolinic acid‐lesioned rodent model of Huntington's disease
Yoon Y, Kim HS, Jeon I, Noh J, Park HJ, Lee S, Park I, Stevanato L, Hicks C, Corteling R, Barker RA, Sinden JD, Song J. Implantation of the clinical‐grade human neural stem cell line, CTX0E03, rescues the behavioral and pathological deficits in the quinolinic acid‐lesioned rodent model of Huntington's disease. Stem Cells 2020, 38: 936-947. PMID: 32374064, PMCID: PMC7496241, DOI: 10.1002/stem.3191.Peer-Reviewed Original ResearchConceptsMedium spiny neuronsNeural stem cell lineHuntington's diseaseQuinolinic acid (QA) lesion rat modelChronic ischemic stroke patientsStriatal medium spiny neuronsCell linesImmortalized neural stem cell linesIschemic stroke patientsDisease-modifying therapiesSignals of efficacyGlial scar formationHost brain tissueHuman neural stem cell lineSignificant behavioral improvementAutosomal dominant neurodegenerative diseaseCTX0E03 cellsEndogenous neurogenesisBDNF expressionGABAergic neuronsHD patientsStroke patientsFluoro-GoldRetrograde labelSpiny neurons
2014
In Vivo Roles of a Patient-Derived Induced Pluripotent Stem Cell Line (HD72-iPSC) in the YAC128 Model of Huntington’s Disease
Jeon I, Choi C, Lee N, Im W, Kim M, Oh SH, Park IH, Kim HS, Song J. In Vivo Roles of a Patient-Derived Induced Pluripotent Stem Cell Line (HD72-iPSC) in the YAC128 Model of Huntington’s Disease. International Journal Of Stem Cells 2014, 7: 43-47. PMID: 24921027, PMCID: PMC4049731, DOI: 10.15283/ijsc.2014.7.1.43.Peer-Reviewed Original ResearchHuntington's diseaseHD pathologyNeural precursorsAvailable therapeutic optionsHD mouse modelsYAC128 transgenic miceSignificant behavioral improvementCAG repeatsVivo roleYAC128 modelGrafted miceTherapeutic optionsGrafted cellsPatient-derived iPSCsMouse modelTransgenic miceBehavioral improvementDiseaseNovel therapeuticsMiceCell linesPluripotent stem cell linePathologyStem cellsNeurodegenerative genetic disease
2011
Altered Hematopoiesis in Trisomy 21 As Revealed Through In Vitro Differentiation of Isogenic Human Pluripotent Cells
MacLean G, Menne T, Park I, Daley G, Orkin S. Altered Hematopoiesis in Trisomy 21 As Revealed Through In Vitro Differentiation of Isogenic Human Pluripotent Cells. Blood 2011, 118: 921. DOI: 10.1182/blood.v118.21.921.921.Peer-Reviewed Original ResearchTrisomy 21Trisomic cellsFetal liverAdvisory CommitteeGATA1 mutationsPre-leukemic conditionAcute myeloid leukemiaTransient myeloproliferative disorderTrisomy 21 resultsCell linesDisomic controlsSomatic mutationsCFU-M coloniesColony forming assaysMultilineage hematopoietic progenitor cellsQuantitative RT-PCRHematopoietic progenitor cellsIL-6Myeloid leukemiaMouse modelMyeloproliferative disordersMegakaryoblastic leukemiaProgenitor populationsMyeloid progenitor populationsCD31/CD34Analysis of Differential Proteomes of Induced Pluripotent Stem Cells by Protein-Based Reprogramming of Fibroblasts
Jin J, Kwon YW, Paek JS, Cho HJ, Yu J, Lee JY, Chu IS, Park IH, Park YB, Kim HS, Kim Y. Analysis of Differential Proteomes of Induced Pluripotent Stem Cells by Protein-Based Reprogramming of Fibroblasts. Journal Of Proteome Research 2011, 10: 977-989. PMID: 21175196, DOI: 10.1021/pr100624f.Peer-Reviewed Original ResearchConceptsInduced pluripotent stem cellsES cell linesPluripotent stem cellsIPS cellsDifferential proteomeGlobal gene expression patternsCell extractsGlobal gene expression analysisCell linesReprogramming of fibroblastsSpecific transcription factorsProtein synthetic machineryPluripotent ES cellsGene expression patternsStem cellsViral transductionMES cell linesGene expression analysisTranscription factorsSynthetic machineryExpression analysisRetroviral integrationES cellsExpression patternsStem cell-based approachesStage-specific signaling through TGFβ family members and WNT regulates patterning and pancreatic specification of human pluripotent stem cells
Nostro MC, Sarangi F, Ogawa S, Holtzinger A, Corneo B, Li X, Micallef SJ, Park IH, Basford C, Wheeler MB, Daley GQ, Elefanty AG, Stanley EG, Keller G. Stage-specific signaling through TGFβ family members and WNT regulates patterning and pancreatic specification of human pluripotent stem cells. Development 2011, 138: 861-871. PMID: 21270052, PMCID: PMC3035090, DOI: 10.1242/dev.055236.Peer-Reviewed Original ResearchConceptsHuman pluripotent stem cellsPluripotent stem cellsTGFβ family membersStem cellsPancreatic lineage cellsEndoderm fateEndoderm populationEndoderm inductionPancreatic specificationInsulin-expressing cellsBMP inhibitionPancreatic lineagePancreatic fateA SignalingInsulin-producing β-cellsGerm layersCanonical WntDevelopmental stagesActivin A signalingFamily membersLineage cellsWntInsulin expressionCell linesSpecific stages
2010
MicroRNA Profiling Reveals Two Distinct p53-Related Human Pluripotent Stem Cell States
Neveu P, Kye MJ, Qi S, Buchholz DE, Clegg DO, Sahin M, Park IH, Kim KS, Daley GQ, Kornblum HI, Shraiman BI, Kosik KS. MicroRNA Profiling Reveals Two Distinct p53-Related Human Pluripotent Stem Cell States. Cell Stem Cell 2010, 7: 671-681. PMID: 21112562, DOI: 10.1016/j.stem.2010.11.012.Peer-Reviewed Original ResearchConceptsInduced pluripotent stem cellsPluripotent stem cell stateEmbryonic stem cellsStem cell stateCell statesDifferentiated cellsStem cellsCell linesPluripotent stem cellsHuman cell linesGene setsMiRNA expression levelsMiR-92Cell line originMicroRNA profilingCancer cell linesLine originMiRNA profilesExpression levelsPluripotencyCancer cellsMiR-141CellsSubtle differencesHESCsRobust Enhancement of Neural Differentiation from Human ES and iPS Cells Regardless of their Innate Difference in Differentiation Propensity
Kim DS, Lee JS, Leem JW, Huh YJ, Kim JY, Kim HS, Park IH, Daley GQ, Hwang DY, Kim DW. Robust Enhancement of Neural Differentiation from Human ES and iPS Cells Regardless of their Innate Difference in Differentiation Propensity. Stem Cell Reviews And Reports 2010, 6: 270-281. PMID: 20376579, DOI: 10.1007/s12015-010-9138-1.Peer-Reviewed Original ResearchConceptsDifferentiation propensityStem cell linesCell lineagesNeural differentiationHuman embryonic stem cell linesEmbryonic stem cell linesCell typesActivin/NodalHuman pluripotent stem cell linesDesirable cell typesSpecific cell typesCell linesModulation of intracellularPluripotent stem cellsPluripotent stem cell lineBMP pathwaySpecific lineagesIPS cellsUndifferentiated cellsLineagesDifferentiation potentialHuman ESStem cellsNeural cellsDifferentiation
2008
Regulatory networks define phenotypic classes of human stem cell lines
Müller FJ, Laurent LC, Kostka D, Ulitsky I, Williams R, Lu C, Park IH, Rao MS, Shamir R, Schwartz PH, Schmidt NO, Loring JF. Regulatory networks define phenotypic classes of human stem cell lines. Nature 2008, 455: 401-405. PMID: 18724358, PMCID: PMC2637443, DOI: 10.1038/nature07213.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsAnimalsArtificial IntelligenceCell DifferentiationCell LineComputational BiologyDatabases, FactualEmbryonic Stem CellsGene Expression ProfilingHumansMiceMultipotent Stem CellsOligonucleotide Array Sequence AnalysisOocytesPhenotypePluripotent Stem CellsProtein BindingStem CellsConceptsStem cell linesStem cellsCell linesCell typesProtein-protein networkHuman stem cell linesDifferent human cell linesSpecific molecular networksPluripotent stem cell lineHuman stem cellsHuman cell linesRegulatory networksTranscriptional profilesBioinformatics analysisPhenotypic classesMolecular networksNeural stem cell lineLimited repertoireTight controlCellsCell samplesPluripotencyEmbryosLinesBroad rangeDisease-Specific Induced Pluripotent Stem Cells
Park IH, Arora N, Huo H, Maherali N, Ahfeldt T, Shimamura A, Lensch MW, Cowan C, Hochedlinger K, Daley GQ. Disease-Specific Induced Pluripotent Stem Cells. Cell 2008, 134: 877-886. PMID: 18691744, PMCID: PMC2633781, DOI: 10.1016/j.cell.2008.07.041.Peer-Reviewed Original ResearchConceptsParkinson's diseaseDown syndromeBecker muscular dystrophyType 1 diabetes mellitusHuntington's diseaseStem cellsPluripotent stem cellsDiabetes mellitusDisease-SpecificLesch-Nyhan syndromeDisease-specific stem cellsDiseased patientsCarrier stateTumor cell linesDiseaseSyndromeMuscular dystrophyAdenosine deaminasePatientsDrug developmentInduced pluripotent stem cellsType IIICell linesDiamond syndromeDisease investigation
2007
Reprogramming of human somatic cells to pluripotency with defined factors
Park IH, Zhao R, West JA, Yabuuchi A, Huo H, Ince TA, Lerou PH, Lensch MW, Daley GQ. Reprogramming of human somatic cells to pluripotency with defined factors. Nature 2007, 451: 141-146. PMID: 18157115, DOI: 10.1038/nature06534.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsCell DifferentiationCell ShapeCells, CulturedDNA MethylationDNA-Binding ProteinsEmbryonic Stem CellsFetusFibroblastsGene Expression ProfilingHMGB ProteinsHomeodomain ProteinsHumansInfant, NewbornKruppel-Like Factor 4Kruppel-Like Transcription FactorsMiceNanog Homeobox ProteinOctamer Transcription Factor-3Pluripotent Stem CellsPromoter Regions, GeneticProto-Oncogene Proteins c-mycSOXB1 Transcription FactorsTeratomaTranscription FactorsTransplantation, HeterologousConceptsEmbryonic stem cellsStem cellsIPS cellsHuman somatic cellsInduced pluripotent stem cellsHuman iPS cellsPluripotent stem cellsHuman primary cellsPatient-specific cellsEarly embryosTranscription factorsSomatic cellsEctopic expressionPluripotencyGene expressionHuman cellsMurine fibroblastsDefined factorsPrimary cellsCell linesDermal fibroblastsCellsInvaluable toolFibroblastsExpression