1999
Role of Cl channels in Cl-dependent Na/H exchange
Rajendran V, Geibel J, Binder H. Role of Cl channels in Cl-dependent Na/H exchange. American Journal Of Physiology 1999, 276: g73-g78. PMID: 9886981, DOI: 10.1152/ajpgi.1999.276.1.g73.Peer-Reviewed Original ResearchConceptsNa/H exchangeCl- channel blockerChannel blockersMicroM DIDSNa/H exchange activityCystic fibrosis transmembrane conductance regulatorIntracellular pH recoveryRat distal colonCl- channelsH exchange activityDistal colonCrypt lumenCrypt cellsAcid loadExchange inhibitorApical membranePH recoveryCl(-) dependenceAnion exchange inhibitorCl- channel activityChannel activityBlockersH exchangePolyclonal antibodiesCl- conductance
1998
Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells
Ikuma M, Binder H, Geibel J. Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells. The Journal Of Membrane Biology 1998, 166: 205-212. PMID: 9843594, DOI: 10.1007/s002329900462.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiportersBariumCell PolarityColonEpithelial CellsH(+)-K(+)-Exchanging ATPaseHomeostasisHydrogen-Ion ConcentrationIntestinal MucosaIntracellular FluidIon TransportMaleOuabainPotassiumPotassium ChannelsPotassium-Hydrogen AntiportersRatsRats, Sprague-DawleySodium-Potassium-Exchanging ATPaseDifferential localization of colonic H+-K+-ATPase isoforms in surface and crypt cells
Rajendran V, Singh S, Geibel J, Binder H. Differential localization of colonic H+-K+-ATPase isoforms in surface and crypt cells. American Journal Of Physiology 1998, 274: g424-g429. PMID: 9486199, DOI: 10.1152/ajpgi.1998.274.2.g424.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBariumColonH(+)-K(+)-Exchanging ATPaseHydrogen-Ion ConcentrationIsoenzymesMaleOuabainPotassiumRatsRats, Sprague-DawleySurface PropertiesConceptsCrypt cellsColonic cryptsRat distal colonSurface cellsSurface epithelial cellsATPase isoformsOuabain-insensitive componentApical membraneDistal colonAcid loadDependent intracellularEpithelial cellsATPase activityCryptsDifferential localizationOuabainATPase alpha subunitCellsAlpha subunitCompelling evidenceIsoformsColonActivityRecovery
1995
An apical permeability barrier to NH3/NH4+ in isolated, perfused colonic crypts.
Singh S, Binder H, Geibel J, Boron W. An apical permeability barrier to NH3/NH4+ in isolated, perfused colonic crypts. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 11573-11577. PMID: 8524806, PMCID: PMC40444, DOI: 10.1073/pnas.92.25.11573.Peer-Reviewed Original ResearchChloride-dependent Na-H Exchange A NOVEL MECHANISM OF SODIUM TRANSPORT IN COLONIC CRYPTS*
Rajendran V, Geibel J, Binder H. Chloride-dependent Na-H Exchange A NOVEL MECHANISM OF SODIUM TRANSPORT IN COLONIC CRYPTS*. Journal Of Biological Chemistry 1995, 270: 11051-11054. PMID: 7744735, DOI: 10.1074/jbc.270.19.11051.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAnimalsAnionsCell MembraneChloridesColonHydrogen-Ion ConcentrationIn Vitro TechniquesIntestinal MucosaKineticsMaleRatsRats, Sprague-DawleySodiumSodium-Hydrogen ExchangersConceptsNa-H exchangeColonic crypt cellsCrypt cellsAcid loadRat distal colonApical membrane vesiclesNovel transport processRate of intracellularDistal colonMM amilorideSodium transportApical membraneGradient stimulationColonic cryptsAmiloride inhibitionSodium movementNovel mechanismOutward gradientCompelling evidenceCellsSodiumUptakePrior studiesColonRecovery
1994
Differential modulation of Na-HCO3 cotransport and Na-H exchange by pH in basolateral membrane vesicles of rat distal colon.
Rajendran V, Binder H. Differential modulation of Na-HCO3 cotransport and Na-H exchange by pH in basolateral membrane vesicles of rat distal colon. Journal Of Biological Chemistry 1994, 269: 156-160. PMID: 8276790, DOI: 10.1016/s0021-9258(17)42328-3.Peer-Reviewed Original ResearchConceptsMembrane vesiclesDifferent cellular functionsBasolateral membrane vesiclesProton concentration gradientCellular functionsIntravesicular protonsApparent KmVesiclesTransport systemNa-H exchangeCotransportDifferential modulationHigh proton concentrationProton concentrationProton stimulationNa-HCO3 cotransportConcentration gradientKmRat distal colonProteinSeparate mechanismsVmax
1993
Bicarbonate-stimulated [14C]butyrate uptake in basolateral membrane vesicles of rat distal colon
Reynolds D, Rajendran V, Binder H. Bicarbonate-stimulated [14C]butyrate uptake in basolateral membrane vesicles of rat distal colon. Gastroenterology 1993, 105: 725-732. PMID: 8359643, DOI: 10.1016/0016-5085(93)90889-k.Peer-Reviewed Original ResearchCl-HCO3 and Cl-OH exchanges mediate Cl uptake in apical membrane vesicles of rat distal colon
Rajendran V, Binder H. Cl-HCO3 and Cl-OH exchanges mediate Cl uptake in apical membrane vesicles of rat distal colon. American Journal Of Physiology 1993, 264: g874-g879. PMID: 8498514, DOI: 10.1152/ajpgi.1993.264.5.g874.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAmilorideAnimalsBicarbonatesBiological TransportBumetanideCarrier ProteinsCell MembraneChloride-Bicarbonate AntiportersChloridesColonDose-Response Relationship, DrugHydrogen-Ion ConcentrationKineticsMembrane ProteinsOxalatesRatsRats, Sprague-Dawley
1991
Apical membrane localization of ouabain-sensitive K(+)-activated ATPase activities in rat distal colon
Del Castillo J, Rajendran V, Binder H. Apical membrane localization of ouabain-sensitive K(+)-activated ATPase activities in rat distal colon. American Journal Of Physiology 1991, 261: g1005-g1011. PMID: 1837421, DOI: 10.1152/ajpgi.1991.261.6.g1005.Peer-Reviewed Original ResearchConceptsRat distal colonOuabain-insensitive fractionDistal colonOuabain-sensitive fractionOuabain-insensitive componentHalf-maximal concentrationApical membraneATPase activityColonic mucosaProximal colonColonActivation curveAbsorptive processesAdenosinetriphosphatase activityApical membrane localizationBasolateral membraneHomogenatesN-ethylmaleimideMucosaActivityOmeprazoleSodium uptake across basolateral membrane of rat distal colon. Evidence for Na-H exchange and Na-anion cotransport.
Rajendran V, Oesterlin M, Binder H. Sodium uptake across basolateral membrane of rat distal colon. Evidence for Na-H exchange and Na-anion cotransport. Journal Of Clinical Investigation 1991, 88: 1379-1385. PMID: 1655829, PMCID: PMC295609, DOI: 10.1172/jci115444.Peer-Reviewed Original ResearchMechanism of short-chain fatty acid uptake by apical membrane vesicles of rat distal colon
Mascolo N, Rajendran V, Binder H. Mechanism of short-chain fatty acid uptake by apical membrane vesicles of rat distal colon. Gastroenterology 1991, 101: 331-338. PMID: 2065907, DOI: 10.1016/0016-5085(91)90008-9.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAnimalsAnionsBicarbonatesBiological Transport, ActiveButyratesButyric AcidCell MembraneColonFatty Acids, VolatileHydrogen-Ion ConcentrationIn Vitro TechniquesIntestinal AbsorptionRatsRats, Inbred Strains
1990
Characterization of Na-H exchange in apical membrane vesicles of rat colon.
Rajendran V, Binder H. Characterization of Na-H exchange in apical membrane vesicles of rat colon. Journal Of Biological Chemistry 1990, 265: 8408-8414. PMID: 2160456, DOI: 10.1016/s0021-9258(19)38902-1.Peer-Reviewed Original ResearchConceptsNa-H exchange
1986
Augmentation of neutral sodium chloride absorption by increased flow rate in rat ileum in vivo.
Harris M, Dobbins J, Binder H. Augmentation of neutral sodium chloride absorption by increased flow rate in rat ileum in vivo. Journal Of Clinical Investigation 1986, 78: 431-438. PMID: 3734099, PMCID: PMC423573, DOI: 10.1172/jci112594.Peer-Reviewed Original ResearchConceptsNeutral sodium chloride absorptionSodium chloride absorptionRat ileumChloride absorptionIntraluminal volumeAddition of furosemideTransmural potential differenceSingle-pass perfusionOutflow tubingIntraluminal pressureSodium absorptionPerfusion mediumIntact animalsElectrolyte absorptionIleumElectrolyte transportRinger's solutionIncreased ratesPerfusion rateRemoval of sodiumStepwise increaseMinimal changesSeparate experimentsUnstirred layer thicknessSolute absorptionPotassium/proton exchange in brush-border membrane of rat ileum
Binder H, Murer H. Potassium/proton exchange in brush-border membrane of rat ileum. The Journal Of Membrane Biology 1986, 91: 77-84. PMID: 3735407, DOI: 10.1007/bf01870217.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological Transport, ActiveHydrogen-Ion ConcentrationIleumKineticsMaleMicrovilliPotassiumRatsRats, Inbred StrainsRubidiumSodium