2023
Pot1b −/− tumors activate G-quadruplex-induced DNA damage to promote telomere hyper-elongation
Takasugi T, Gu P, Liang F, Staco I, Chang S. Pot1b −/− tumors activate G-quadruplex-induced DNA damage to promote telomere hyper-elongation. Nucleic Acids Research 2023, 51: 9227-9247. PMID: 37560909, PMCID: PMC10516629, DOI: 10.1093/nar/gkad648.Peer-Reviewed Original ResearchConceptsDNA damage responseDamage responseReplication protein A (RPA) complexDependent DNA damage responseTelomere length homeostasisTelomere maintenance mechanismLength homeostasisTelomerase recruitmentPOT1 proteinsHuman POT1Mouse genomeLength maintenanceFunction disruptsReplicative immortalityTelomeresPOT1 mutationsDNA damageHuman cancersLonger telomeresPOT1bMaintenance mechanismsSerial transplantationA complexesSimilar mechanismMutations
2020
The DNA-binding activity of USP1-associated factor 1 is required for efficient RAD51-mediated homologous DNA pairing and homology-directed DNA repair
Liang F, Miller AS, Tang C, Maranon D, Williamson EA, Hromas R, Wiese C, Zhao W, Sung P, Kupfer GM. The DNA-binding activity of USP1-associated factor 1 is required for efficient RAD51-mediated homologous DNA pairing and homology-directed DNA repair. Journal Of Biological Chemistry 2020, 295: 8186-8194. PMID: 32350107, PMCID: PMC7294083, DOI: 10.1074/jbc.ra120.013714.Peer-Reviewed Original ResearchConceptsHomologous DNA pairingDNA-binding activityDNA pairingDNA repairDNA bindingFanconi anemia complementation group D2 proteinDamage repairHomology-directed DNA repairRAD51 recombinase activityFanconi anemia pathwayDNA damage sensitivityChromosome damage repairHR-mediated DNA repairDNA damage responseDNA damage repairHomologous recombination pathwayFactor 1FANCD2 deubiquitinationRole of DNAEfficient RAD51Deubiquitinase complexRecombinase RAD51Damage responseUAF1D2 protein
2019
Chromatin‐bound cGAS is an inhibitor of DNA repair and hence accelerates genome destabilization and cell death
Jiang H, Xue X, Panda S, Kawale A, Hooy RM, Liang F, Sohn J, Sung P, Gekara NO. Chromatin‐bound cGAS is an inhibitor of DNA repair and hence accelerates genome destabilization and cell death. The EMBO Journal 2019, 38: embj2019102718. PMID: 31544964, PMCID: PMC6826206, DOI: 10.15252/embj.2019102718.Peer-Reviewed Original ResearchConceptsCyclic GMP-AMP synthaseHomologous recombinationDNA repairGenome destabilizationCell deathChromatin-binding proteinsGMP-AMP synthaseSsDNA filamentsGenome integrityGenomic stressInnate immune sensorsHR repairChromosomal rearrangementsStrand invasionRegulatory mechanismsUnknown roleCytosolic innate immune sensorsCell survivalTemplate dsDNAImmune sensorsBone marrow cellsMicronuclei generationOutcome of infectionMarrow cellsInnate immune activationDNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response
Liang F, Miller AS, Longerich S, Tang C, Maranon D, Williamson EA, Hromas R, Wiese C, Kupfer GM, Sung P. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nature Communications 2019, 10: 2849. PMID: 31253762, PMCID: PMC6599204, DOI: 10.1038/s41467-019-10408-5.Peer-Reviewed Original ResearchConceptsFANCD2 deubiquitinationDNA damage response pathwayFanconi anemiaUSP1-UAF1 complexDNA damage toleranceDNA damage responseDamage response pathwayImportance of DNAGenome repairDamage responseResponse pathwaysUAF1DNA requirementsCellular settingsFA pathwayDNA bindingDownstream eventsDeubiquitinationRAD51AP1DNA crosslinksBiochemical systemsBone marrow failureChromosomal lesionsMultigenic diseasesMultifaceted roleBinding of FANCI-FANCD2 Complex to RNA and R-Loops Stimulates Robust FANCD2 Monoubiquitination
Liang Z, Liang F, Teng Y, Chen X, Liu J, Longerich S, Rao T, Green AM, Collins NB, Xiong Y, Lan L, Sung P, Kupfer GM. Binding of FANCI-FANCD2 Complex to RNA and R-Loops Stimulates Robust FANCD2 Monoubiquitination. Cell Reports 2019, 26: 564-572.e5. PMID: 30650351, PMCID: PMC6350941, DOI: 10.1016/j.celrep.2018.12.084.Peer-Reviewed Original ResearchConceptsR-loopsCo-transcriptional R-loopsFANCI-FANCD2 complexFanconi anemiaR-loop structuresSeries of RNADNA replicative stressGuanine-rich sequencesFANCD2 monoubiquitinationGenomic lociID2 complexReplicative stressFA cellsRNA transcriptionFA pathwayComplex bindsDNA hybridsCancer predispositionBone marrow failureDNA damageRNAMonoubiquitinationDevelopmental abnormalitiesMarrow failureSsRNA
2017
BRCA1–BARD1 promotes RAD51-mediated homologous DNA pairing
Zhao W, Steinfeld JB, Liang F, Chen X, Maranon DG, Jian Ma C, Kwon Y, Rao T, Wang W, Sheng C, Song X, Deng Y, Jimenez-Sainz J, Lu L, Jensen RB, Xiong Y, Kupfer GM, Wiese C, Greene EC, Sung P. BRCA1–BARD1 promotes RAD51-mediated homologous DNA pairing. Nature 2017, 550: 360-365. PMID: 28976962, PMCID: PMC5800781, DOI: 10.1038/nature24060.Peer-Reviewed Original ResearchAmino Acid SequenceBase PairingBRCA1 ProteinBRCA2 ProteinChromosome PairingFanconi Anemia Complementation Group N ProteinGenes, BRCA1Genes, BRCA2HumansMultiprotein ComplexesMutationProtein BindingRad51 RecombinaseRecombinational DNA RepairSequence Homology, Nucleic AcidTemplates, GeneticTumor Suppressor ProteinsUbiquitin-Protein Ligases
2016
Promotion of RAD51-Mediated Homologous DNA Pairing by the RAD51AP1-UAF1 Complex
Liang F, Longerich S, Miller AS, Tang C, Buzovetsky O, Xiong Y, Maranon DG, Wiese C, Kupfer GM, Sung P. Promotion of RAD51-Mediated Homologous DNA Pairing by the RAD51AP1-UAF1 Complex. Cell Reports 2016, 15: 2118-2126. PMID: 27239033, PMCID: PMC5381662, DOI: 10.1016/j.celrep.2016.05.007.Peer-Reviewed Original ResearchConceptsHomologous recombinationFanconi anemia DNA damage response pathwayDNA damage response pathwaySmall ubiquitin-like modifierDNA damage hypersensitivityHomologous DNA pairingSUMO-interacting motifDamage response pathwayUbiquitin-like modifierFANCD2 ubiquitinationGenome maintenanceComplex formationNucleoprotein intermediatesRAD51 recombinaseDNA pairingProtein complexesBinds DNAAccessory factorsResponse pathwaysHomologous DNADNA repairLike domainUAF1RAD51RAD51AP1
2015
NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability
Parplys AC, Zhao W, Sharma N, Groesser T, Liang F, Maranon DG, Leung SG, Grundt K, Dray E, Idate R, Østvold AC, Schild D, Sung P, Wiese C. NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Research 2015, 43: 9817-9834. PMID: 26323318, PMCID: PMC4787752, DOI: 10.1093/nar/gkv859.Peer-Reviewed Original ResearchConceptsHomologous recombinationDNA replication fork progressionChromatin-associated proteinsVertebrate-specific proteinReplication fork progressionDNA damage responseDNA repair pathwaysNew replication originsNumber of proteinsExtensive sequence homologyImpairs DNA repairGenome stabilityLimited functional dataChromosome stabilityFork progressionDamage responseReplication originsHR pathwayPhenotypic effectsDNA repairRepair pathwaysSequence homologyTumor suppressionSame DNANUCKS1