2023
Pot1b −/− tumors activate G-quadruplex-induced DNA damage to promote telomere hyper-elongation
Takasugi T, Gu P, Liang F, Staco I, Chang S. Pot1b −/− tumors activate G-quadruplex-induced DNA damage to promote telomere hyper-elongation. Nucleic Acids Research 2023, 51: 9227-9247. PMID: 37560909, PMCID: PMC10516629, DOI: 10.1093/nar/gkad648.Peer-Reviewed Original ResearchConceptsDNA damage responseDamage responseReplication protein A (RPA) complexDependent DNA damage responseTelomere length homeostasisTelomere maintenance mechanismLength homeostasisTelomerase recruitmentPOT1 proteinsHuman POT1Mouse genomeLength maintenanceFunction disruptsReplicative immortalityTelomeresPOT1 mutationsDNA damageHuman cancersLonger telomeresPOT1bMaintenance mechanismsSerial transplantationA complexesSimilar mechanismMutations
2020
The DNA-binding activity of USP1-associated factor 1 is required for efficient RAD51-mediated homologous DNA pairing and homology-directed DNA repair
Liang F, Miller AS, Tang C, Maranon D, Williamson EA, Hromas R, Wiese C, Zhao W, Sung P, Kupfer GM. The DNA-binding activity of USP1-associated factor 1 is required for efficient RAD51-mediated homologous DNA pairing and homology-directed DNA repair. Journal Of Biological Chemistry 2020, 295: 8186-8194. PMID: 32350107, PMCID: PMC7294083, DOI: 10.1074/jbc.ra120.013714.Peer-Reviewed Original ResearchConceptsHomologous DNA pairingDNA-binding activityDNA pairingDNA repairDNA bindingFanconi anemia complementation group D2 proteinDamage repairHomology-directed DNA repairRAD51 recombinase activityFanconi anemia pathwayDNA damage sensitivityChromosome damage repairHR-mediated DNA repairDNA damage responseDNA damage repairHomologous recombination pathwayFactor 1FANCD2 deubiquitinationRole of DNAEfficient RAD51Deubiquitinase complexRecombinase RAD51Damage responseUAF1D2 protein
2019
DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response
Liang F, Miller AS, Longerich S, Tang C, Maranon D, Williamson EA, Hromas R, Wiese C, Kupfer GM, Sung P. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nature Communications 2019, 10: 2849. PMID: 31253762, PMCID: PMC6599204, DOI: 10.1038/s41467-019-10408-5.Peer-Reviewed Original ResearchConceptsFANCD2 deubiquitinationDNA damage response pathwayFanconi anemiaUSP1-UAF1 complexDNA damage toleranceDNA damage responseDamage response pathwayImportance of DNAGenome repairDamage responseResponse pathwaysUAF1DNA requirementsCellular settingsFA pathwayDNA bindingDownstream eventsDeubiquitinationRAD51AP1DNA crosslinksBiochemical systemsBone marrow failureChromosomal lesionsMultigenic diseasesMultifaceted role
2015
NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability
Parplys AC, Zhao W, Sharma N, Groesser T, Liang F, Maranon DG, Leung SG, Grundt K, Dray E, Idate R, Østvold AC, Schild D, Sung P, Wiese C. NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Research 2015, 43: 9817-9834. PMID: 26323318, PMCID: PMC4787752, DOI: 10.1093/nar/gkv859.Peer-Reviewed Original ResearchConceptsHomologous recombinationDNA replication fork progressionChromatin-associated proteinsVertebrate-specific proteinReplication fork progressionDNA damage responseDNA repair pathwaysNew replication originsNumber of proteinsExtensive sequence homologyImpairs DNA repairGenome stabilityLimited functional dataChromosome stabilityFork progressionDamage responseReplication originsHR pathwayPhenotypic effectsDNA repairRepair pathwaysSequence homologyTumor suppressionSame DNANUCKS1