2006
Coinfection with Borrelia burgdorferi sensu stricto and Borrelia garinii alters the course of murine Lyme borreliosis
Hovius JW, Li X, Ramamoorthi N, Van Dam AP, Barthold SW, Van Der Poll T, Speelman P, Fikrig E. Coinfection with Borrelia burgdorferi sensu stricto and Borrelia garinii alters the course of murine Lyme borreliosis. Pathogens And Disease 2006, 49: 224-234. PMID: 17328756, DOI: 10.1111/j.1574-695x.2006.00177.x.Peer-Reviewed Original ResearchConceptsMurine Lyme borreliosisB. burgdorferi sensu strictoBurgdorferi sensu strictoLyme borreliosisBorrelia burgdorferi sensu strictoEffect of coinfectionCoinfected miceBorrelia speciesSimultaneous infectionMiceCoinfectionBorreliosisB. gariniiBorrelia gariniiReservoir hostsGariniiI. ricinus populationsSpirochetesHigher numberArthritisPreferential maintenanceInfectionPrevalenceSpirochetemia
2005
Association of Linear Plasmid 28-1 with an Arthritic Phenotype of Borrelia burgdorferi
Xu Q, Seemanapalli SV, Lomax L, McShan K, Li X, Fikrig E, Liang FT. Association of Linear Plasmid 28-1 with an Arthritic Phenotype of Borrelia burgdorferi. Infection And Immunity 2005, 73: 7208-7215. PMID: 16239515, PMCID: PMC1273894, DOI: 10.1128/iai.73.11.7208-7215.2005.Peer-Reviewed Original ResearchConceptsSevere combined immunodeficiencyArthritic phenotypeLp28-1Linear plasmid 28Borrelia burgdorferiArthritic pathologySevere arthritisSpirochete burdenSCID miceJoint tissuesLoss of plasmidsCombined immunodeficiencyPlasmid content analysisLyme disease spirocheteInfectivity studiesPlasmid patternsDecreased infectivityVirulence factorsFurther studiesMiceBurgdorferiBacterial quantificationPathogenicity studiesTissuePhenotype
2002
DNA Microarray Assessment of Putative Borrelia burgdorferi Lipoprotein Genes
Liang FT, Nelson FK, Fikrig E. DNA Microarray Assessment of Putative Borrelia burgdorferi Lipoprotein Genes. Infection And Immunity 2002, 70: 3300-3303. PMID: 12011030, PMCID: PMC128019, DOI: 10.1128/iai.70.6.3300-3303.2002.Peer-Reviewed Original ResearchAntibodies to granulocytic ehrlichiae in cattle from Connecticut
Magnarelli LA, Ijdo JW, Sherman B, Bushmich SL, Levy SA, Fikrig E. Antibodies to granulocytic ehrlichiae in cattle from Connecticut. Journal Of Medical Microbiology 2002, 51: 326-331. PMID: 11926738, DOI: 10.1099/0022-1317-51-4-326.Peer-Reviewed Original ResearchConceptsIndirect fluorescent antibody
2001
Borrelia burgdorferi-Induced Inflammation Facilitates Spirochete Adaptation and Variable Major Protein-Like Sequence Locus Recombination
Anguita J, Thomas V, Samanta S, Persinski R, Hernanz C, Barthold S, Fikrig E. Borrelia burgdorferi-Induced Inflammation Facilitates Spirochete Adaptation and Variable Major Protein-Like Sequence Locus Recombination. The Journal Of Immunology 2001, 167: 3383-3390. PMID: 11544329, PMCID: PMC4309988, DOI: 10.4049/jimmunol.167.6.3383.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAnimalsAntibodies, BacterialAntigens, BacterialAntigens, SurfaceBacterial ProteinsBase SequenceBorrelia burgdorferiCD4-Positive T-LymphocytesDNA, BacterialGene Expression RegulationImmune SeraImmunocompetenceInflammationInterferon-gammaInterleukin-12LipoproteinsLyme DiseaseMiceMice, Inbred C3HMice, KnockoutMolecular Sequence DataReceptors, InterferonRecombination, GeneticSequence AlignmentSequence Homology, Nucleic AcidConceptsImmunocompetent miceDeficient miceB. burgdorferi N40IFN-gammaRMurine immune responseIFN-gamma-mediated responsesIFN-gamma-mediated signalsSpirochetal burdensSpirochete clearanceIL-12Immune responseIFN-gammaControl animalsDifferential immunoscreeningMice resultsMiceVariable major proteinsRT-PCRVivo adaptationB. burgdorferiClearanceBorrelia burgdorferi gene expressionB. burgdorferi survivalAdministrationVivoCoinfection with Borrelia burgdorferi and the Agent of Human Granulocytic Ehrlichiosis Alters Murine Immune Responses, Pathogen Burden, and Severity of Lyme Arthritis
Thomas V, Anguita J, Barthold S, Fikrig E. Coinfection with Borrelia burgdorferi and the Agent of Human Granulocytic Ehrlichiosis Alters Murine Immune Responses, Pathogen Burden, and Severity of Lyme Arthritis. Infection And Immunity 2001, 69: 3359-3371. PMID: 11292759, PMCID: PMC98295, DOI: 10.1128/iai.69.5.3359-3371.2001.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisLyme arthritisDual infectionBacterial burdenImmune responseTumor necrosis factor-alpha levelsB. burgdorferiElevated IL-6 levelsNecrosis factor-alpha levelsGranulocytic ehrlichiosisIL-6 levelsAgent of HGEMurine immune responseBorrelia burgdorferiTick-borne illnessMurine Lyme arthritisSevere Lyme arthritisCoinfection of miceIFN-gamma receptor expressionInfected miceInterleukin-12Receptor expressionGamma interferonArthritisPathogen burden
2000
Detection of Ehrlichia chaffeensis DNA inAmblyomma americanum Ticks in Connecticut and Rhode Island
Ijdo J, Wu C, Magnarelli L, Stafford K, Anderson J, Fikrig E. Detection of Ehrlichia chaffeensis DNA inAmblyomma americanum Ticks in Connecticut and Rhode Island. Journal Of Clinical Microbiology 2000, 38: 4655-4656. PMID: 11101616, PMCID: PMC87657, DOI: 10.1128/jcm.38.12.4655-4656.2000.Peer-Reviewed Original Research
1999
Serologic testing of horses for granulocytic ehrlichiosis, using indirect fluorescent antibody staining and immunoblot analysis.
Magnarelli L, Van Andel A, Ijdo J, Heimer R, Fikrig E. Serologic testing of horses for granulocytic ehrlichiosis, using indirect fluorescent antibody staining and immunoblot analysis. American Journal Of Veterinary Research 1999, 60: 631-5. PMID: 10328436, DOI: 10.2460/ajvr.1999.60.05.631.Peer-Reviewed Original ResearchINFECTIONS OF GRANULOCYTIC EHRLICHIAE AND BORRELIA BURGDORFERI IN WHITE-TAILED DEER IN CONNECTICUT
Magnarelli L, IJdo J, Stafford K, Fikrig E. INFECTIONS OF GRANULOCYTIC EHRLICHIAE AND BORRELIA BURGDORFERI IN WHITE-TAILED DEER IN CONNECTICUT. Journal Of Wildlife Diseases 1999, 35: 266-274. PMID: 10231753, DOI: 10.7589/0090-3558-35.2.266.Peer-Reviewed Original Research
1997
Immunization against the agent of human granulocytic ehrlichiosis in a murine model.
Sun W, IJdo JW, Telford SR, Hodzic E, Zhang Y, Barthold SW, Fikrig E. Immunization against the agent of human granulocytic ehrlichiosis in a murine model. Journal Of Clinical Investigation 1997, 100: 3014-3018. PMID: 9399947, PMCID: PMC508513, DOI: 10.1172/jci119855.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisGranulocytic ehrlichiosisC3H/HeN miceAgent of HGEMimic human diseaseTick-borne infectionsTransient neutropeniaHeN miceMurine modelPolymorphonuclear leukocytesTick-borne pathogensAoHGEMiceImmunityEhrlichiosisSyringe inoculationHuman diseasesAgentsNeutropeniaImmunizationInfectionLeukocytesDiseaseAntibodies
1996
Novel Borrelia burgdorferi isolates from Ixodes scapularis and Ixodes dentatus ticks feeding on humans
Anderson JF, Flavell RA, Magnarelli LA, Barthold SW, Kantor FS, Wallich R, Persing DH, Mathiesen D, Fikrig E. Novel Borrelia burgdorferi isolates from Ixodes scapularis and Ixodes dentatus ticks feeding on humans. Journal Of Clinical Microbiology 1996, 34: 524-529. PMID: 8904407, PMCID: PMC228839, DOI: 10.1128/jcm.34.3.524-529.1996.Peer-Reviewed Original ResearchConceptsLate Lyme diseaseBorrelia burgdorferiScapularis ticksPulsed-field gel electrophoresis patternsWhite-footed miceIxodes scapularis ticksB. burgdorferi sensu strictoI. scapularis ticksBurgdorferi sensu strictoLyme diseaseMonoclonal antibodiesGel electrophoresis patternsI. scapularisRestriction fragment length polymorphismFragment length polymorphismMicePrevious isolatesZS7Strain B31
1995
Borrelia burgdorferi genes selectively expressed in the infected host.
Suk K, Das S, Sun W, Jwang B, Barthold SW, Flavell RA, Fikrig E. Borrelia burgdorferi genes selectively expressed in the infected host. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 4269-4273. PMID: 7753795, PMCID: PMC41925, DOI: 10.1073/pnas.92.10.4269.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, BacterialAntigen-Antibody ReactionsAntigens, BacterialBacterial VaccinesBase SequenceBlotting, NorthernBorrelia burgdorferi GroupDNA PrimersDNA, BacterialGene ExpressionGene Transfer TechniquesGenes, BacterialGenomic LibraryImmunizationMiceMice, Inbred C3HMolecular Sequence DataPolymerase Chain ReactionSequence Homology, Amino AcidConceptsInfected hostRNA PCRDifferential screeningScreening strategyExpression libraryB. burgdorferiSelective expressionNorthern blot analysisBlot analysisMiceBorrelia burgdorferi genesB. burgdorferi genesVivoGene transfer systemMicrobial genesScreening methodPathogenic microorganismsScreeningGenesAntigenVaccination against Lyme disease caused by diverse Borrelia burgdorferi.
Fikrig E, Telford SR, Wallich R, Chen M, Lobet Y, Matuschka FR, Kimsey RB, Kantor FS, Barthold SW, Spielman A, Flavell RA. Vaccination against Lyme disease caused by diverse Borrelia burgdorferi. Journal Of Experimental Medicine 1995, 181: 215-221. PMID: 7807004, PMCID: PMC2191810, DOI: 10.1084/jem.181.1.215.Peer-Reviewed Original ResearchConceptsLyme diseaseB. burgdorferiVivo protection studiesBorrelia burgdorferi sensu latoSurface protein AOuter surface protein AVector-borne infectionsVaccine efficacyBurgdorferi sensu latoChallenge inoculumSpirochetal agentInfectionBorrelia burgdorferiDiseaseBurgdorferiOspAClassification systemProtection studiesProtein ADifferent geographic regionsSyringe injectionGeographic regionsVaccinationImmunizationVaccine
1994
A chromosomal Borrelia burgdorferi gene encodes a 22-kilodalton lipoprotein, P22, that is serologically recognized in Lyme disease
Lam TT, Nguyen TP, Fikrig E, Flavell RA. A chromosomal Borrelia burgdorferi gene encodes a 22-kilodalton lipoprotein, P22, that is serologically recognized in Lyme disease. Journal Of Clinical Microbiology 1994, 32: 876-883. PMID: 8027338, PMCID: PMC263156, DOI: 10.1128/jcm.32.4.876-883.1994.Peer-Reviewed Original ResearchConceptsLate-stage diseaseLyme diseaseOuter surface proteinsEarly-stage diseaseExamination of seraErythema migransSurface proteinsPulsed-field gel electrophoresisPatientsEtiologic agentDiseaseBorrelia burgdorferiB. burgdorferiAntibodiesLipoproteinBorrelia burgdorferi genesSerumBurgdorferiShort amino terminusTarget Imbalance: Disparity of Borrelia burgdorferi Genetic Material in Synovial Fluid from Lyme Arthritis Patients
Persing D, Rutledge B, Rys P, Podzorski D, Mitchell P, Ree K, Liu B, Fikrig E, Malawista S. Target Imbalance: Disparity of Borrelia burgdorferi Genetic Material in Synovial Fluid from Lyme Arthritis Patients. The Journal Of Infectious Diseases 1994, 169: 668-672. PMID: 8158048, DOI: 10.1093/infdis/169.3.668.Peer-Reviewed Original ResearchConceptsLyme arthritisClinical specimensLyme arthritis patientsUnderlying pathogenetic mechanismsPolymerase chain reaction studiesSynovial fluid specimensB. burgdorferi infectionArthritis patientsSynovial inflammationPathogenetic mechanismsBurgdorferi infectionPhysiologic imbalanceSynovial fluidFluid specimensEtiologic agentLate manifestationLyme diseaseCultured organismsBorrelia burgdorferiArthritisPatientsDNA reactivityAnalytic sensitivity