2016
Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection
Conway MJ, Londono-Renteria B, Troupin A, Watson AM, Klimstra WB, Fikrig E, Colpitts TM. Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection. PLOS Neglected Tropical Diseases 2016, 10: e0004941. PMID: 27632170, PMCID: PMC5025043, DOI: 10.1371/journal.pntd.0004941.Peer-Reviewed Original ResearchConceptsSalivary gland extractsDengue virus infectionD7 proteinDengue virus type 1Virus type 1DENV envelope proteinAegypti salivaLymph nodesDENV infectionDENV virionsVirus infectionRelevant arbovirusesType 1InfectionHost skinBlood feedingEnvelope proteinNovel roleBinding assaysSalivaAedes aegyptiGland extractsPrimary vectorHPLC fractionsFeeding
2012
Humoral immune responses in humanized BLT mice immunized with West Nile virus and HIV‐1 envelope proteins are largely mediated via human CD5+ B cells
Biswas S, Chang H, Sarkis P, Fikrig E, Zhu Q, Marasco W. Humoral immune responses in humanized BLT mice immunized with West Nile virus and HIV‐1 envelope proteins are largely mediated via human CD5+ B cells. Immunology 2012, 136: 361-361. PMCID: PMC3385036, DOI: 10.1111/j.1365-2567.2012.03586.x.Peer-Reviewed Original Research
2011
Humoral immune responses in humanized BLT mice immunized with West Nile virus and HIV‐1 envelope proteins are largely mediated via human CD5+ B cells
Biswas S, Chang H, Sarkis PT, Fikrig E, Zhu Q, Marasco WA. Humoral immune responses in humanized BLT mice immunized with West Nile virus and HIV‐1 envelope proteins are largely mediated via human CD5+ B cells. Immunology 2011, 134: 419-433. PMID: 22044090, PMCID: PMC3230796, DOI: 10.1111/j.1365-2567.2011.03501.x.Peer-Reviewed Original ResearchConceptsHumoral immune responseImmune responseBLT miceB cellsB lymphocytesVaccine-induced humoral immune responsesVaccine-induced immune responsesVaccine-induced antibody responsesNon-obese diabetic/T cell-independent pathwayHIV-1 envelope proteinSecondary immune responseSingle intramuscular immunizationSpecific IgM levelsViral envelope antigensB cell subsetsAntigen-specific human antibodiesFollicular B cellsCytokines/growth factorsEnvelope proteinHuman B lymphocytesHuman lymphoid systemMature follicular B cellsWest Nile virus envelope proteinHuman T lymphocytesUse of a tandem affinity purification assay to detect interactions between West Nile and dengue viral proteins and proteins of the mosquito vector
Colpitts TM, Cox J, Nguyen A, Feitosa F, Krishnan MN, Fikrig E. Use of a tandem affinity purification assay to detect interactions between West Nile and dengue viral proteins and proteins of the mosquito vector. Virology 2011, 417: 179-187. PMID: 21700306, PMCID: PMC3166580, DOI: 10.1016/j.virol.2011.06.002.Peer-Reviewed Original ResearchConceptsWest Nile virus infectionWest NileMosquito vectorsWest Nile virus envelope proteinMosquito proteinsSignificant morbidityFlavivirus infectionDengue viral proteinsVirus envelope proteinVirus infectionMosquito factorsDengue virusNovel targetInfectionMosquito cellsDengueEnvelope proteinMyosin light chain kinaseViral proteinsFlavivirusesLight chain kinasePI3-kinaseChain kinaseNS2B proteinCells
2009
Fusion Loop Peptide of the West Nile Virus Envelope Protein Is Essential for Pathogenesis and Is Recognized by a Therapeutic Cross-Reactive Human Monoclonal Antibody
Sultana H, Foellmer HG, Neelakanta G, Oliphant T, Engle M, Ledizet M, Krishnan MN, Bonafé N, Anthony KG, Marasco WA, Kaplan P, Montgomery RR, Diamond MS, Koski RA, Fikrig E. Fusion Loop Peptide of the West Nile Virus Envelope Protein Is Essential for Pathogenesis and Is Recognized by a Therapeutic Cross-Reactive Human Monoclonal Antibody. The Journal Of Immunology 2009, 183: 650-660. PMID: 19535627, PMCID: PMC3690769, DOI: 10.4049/jimmunol.0900093.Peer-Reviewed Original ResearchConceptsWest Nile virus envelope proteinWest Nile virusVirus envelope proteinDengue virusCross-reactive human monoclonal antibodiesBlood-brain barrier permeabilityEnvelope proteinWest Nile virus infectionNeutralization escape variantsNile virusWest Nile encephalitisNeutralization escape mutantsHuman monoclonal antibodyFatal neurological diseaseParental West Nile virusFusion loopEscape variantsInflammatory responseBarrier permeabilityLethal encephalitisMAb11Virus infectionHuman mAbsEscape mutantsNeurological diseases
2007
Antibodies Targeting Linear Determinants of the Envelope Protein Protect Mice against West Nile Virus
Ledizet M, Kar K, Foellmer HG, Bonafé N, Anthony KG, Gould LH, Bushmich SL, Fikrig E, Koski RA. Antibodies Targeting Linear Determinants of the Envelope Protein Protect Mice against West Nile Virus. The Journal Of Infectious Diseases 2007, 196: 1741-1748. PMID: 18190253, DOI: 10.1086/523654.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, ViralAntibody AffinityChlorocebus aethiopsDrosophilaEpitopesFemaleFluorescent Antibody TechniqueHorsesImmunization, PassiveImmunoglobulinsMiceMice, Inbred C3HMolecular Sequence DataPeptide FragmentsVero CellsViral Envelope ProteinsWest Nile FeverWest Nile virusConceptsE proteinMultiple flavivirus infectionsVirus-neutralizing antibodiesE protein epitopesFlavivirus envelope proteinProtect miceWest Nile virusFlavivirus infectionLethal challengeProtective antibodiesWest Nile virus E proteinProtective epitopesVirus E proteinRelated flavivirusesImmunoglobulinAntibodiesNile virusLinear determinantsEpitopesEnvelope proteinProtein epitopesMiceHost cell membraneWNVPeptidesWest Nile Virus Envelope Protein Inhibits dsRNA-Induced Innate Immune Responses
Arjona A, Ledizet M, Anthony K, Bonafé N, Modis Y, Town T, Fikrig E. West Nile Virus Envelope Protein Inhibits dsRNA-Induced Innate Immune Responses. The Journal Of Immunology 2007, 179: 8403-8409. PMID: 18056386, DOI: 10.4049/jimmunol.179.12.8403.Peer-Reviewed Original ResearchConceptsWest Nile virusInnate immune responseReceptor-interacting protein 1Immune responseMajor structural proteinVirus-associated molecular patternsDipteran cellsViral replication intermediatesRNA helicasesAdaptor molecule TRIFReplication intermediatesStructural proteinsWNV envelope proteinGlycosylation patternsMolecular patternsAntiviral stateGlycosylation profileProtein 1Murine macrophagesProinflammatory cytokinesCytokine productionImmunosuppressive effectsDsRNAImmune cellsEnvelope protein
2006
Antiviral Peptides Targeting the West Nile Virus Envelope Protein
Bai F, Town T, Pradhan D, Cox J, Ashish, Ledizet M, Anderson JF, Flavell RA, Krueger JK, Koski RA, Fikrig E. Antiviral Peptides Targeting the West Nile Virus Envelope Protein. Journal Of Virology 2006, 81: 2047-2055. PMID: 17151121, PMCID: PMC1797586, DOI: 10.1128/jvi.01840-06.Peer-Reviewed Original ResearchConceptsWest Nile virusMurine blood-brain barrierEnvelope proteinBlood-brain barrierPeptide 9West Nile encephalitisWNV envelope proteinCentral nervous systemWest Nile virus envelope proteinCDNA phage display libraryBrain parenchymaVirus envelope proteinHuman encephalitisViral envelope proteinsWNV infectionControl animalsPeptide-1Nervous systemRelated flavivirusesDengue virusAntiviral activityNew therapeuticsInhibition concentrationAntiviral peptidesNile virus
2005
Protective and Therapeutic Capacity of Human Single-Chain Fv-Fc Fusion Proteins against West Nile Virus
Gould LH, Sui J, Foellmer H, Oliphant T, Wang T, Ledizet M, Murakami A, Noonan K, Lambeth C, Kar K, Anderson JF, de Silva AM, Diamond MS, Koski RA, Marasco WA, Fikrig E. Protective and Therapeutic Capacity of Human Single-Chain Fv-Fc Fusion Proteins against West Nile Virus. Journal Of Virology 2005, 79: 14606-14613. PMID: 16282460, PMCID: PMC1287547, DOI: 10.1128/jvi.79.23.14606-14613.2005.Peer-Reviewed Original ResearchConceptsWest Nile virus infectionWest Nile virusVirus infectionNile virusLethal West Nile virus infectionFc fusion proteinEnvelope proteinShort-term prophylaxisRecombinant human scFvsHuman single-chain FvWest Nile virus envelope proteinVariable region antibodiesPassive immunizationFlavivirus infectionVirus envelope proteinHuman vaccinesDay 1Dengue virusHuman scFvRegion antibodiesInfectionTherapeutic capacityMiceSerotype 2Single-chain Fv
2004
Immunity to West Nile virus
Wang T, Fikrig E. Immunity to West Nile virus. Current Opinion In Immunology 2004, 16: 519-523. PMID: 15245749, DOI: 10.1016/j.coi.2004.05.008.Peer-Reviewed Original ResearchConceptsWN virus infectionWest Nile virusVirus infectionImportant public health concernWN virusNile virusPublic health concernActive immunizationPassive transferEffective vaccineT cellsMurine susceptibilityHealth concernExperimental modelEnvelope proteinVirusInfectionImmunityRecent studiesImmunotherapyImmunizationPathogenesisTherapyVaccine
2002
A Recombinant Envelope Protein-Based Enzyme-Linked Immunosorbent Assay for West Nile Virus Serodiagnosis
Wang T, Magnarelli LA, Anderson JF, Gould LH, Bushmich SL, Wong SJ, Fikrig E. A Recombinant Envelope Protein-Based Enzyme-Linked Immunosorbent Assay for West Nile Virus Serodiagnosis. Vector-Borne And Zoonotic Diseases 2002, 2: 105-109. PMID: 12653304, DOI: 10.1089/153036602321131904.Peer-Reviewed Original Research
2001
West Nile Virus Envelope Protein
WANG T, ANDERSON J, MAGNARELLI L, BUSHMICH S, WONG S, KOSKI R, FIKRIG E. West Nile Virus Envelope Protein. Annals Of The New York Academy Of Sciences 2001, 951: 325-327. PMID: 11797789, DOI: 10.1111/j.1749-6632.2001.tb02708.x.Peer-Reviewed Original ResearchConceptsWest Nile virus envelope proteinVirus envelope proteinWest Nile virus infectionRole of antibodiesE proteinEnvelope proteinWest Nile virusPassive immunizationHumoral responseWest Nile virus E proteinVirus infectionVirus E proteinNile virusProtein serumInfectionAntibodiesRecombinant formPatientsImmunizationProteinMiceDiagnosisImmunitySerumImmunization of Mice Against West Nile Virus with Recombinant Envelope Protein
Wang T, Anderson J, Magnarelli L, Wong S, Koski R, Fikrig E. Immunization of Mice Against West Nile Virus with Recombinant Envelope Protein. The Journal Of Immunology 2001, 167: 5273-5277. PMID: 11673542, DOI: 10.4049/jimmunol.167.9.5273.Peer-Reviewed Original ResearchConceptsWN virus infectionRecombinant E proteinWest Nile virusVirus infectionC3H/HeN miceE proteinNile virusImmunization of miceRecombinant envelope proteinsPassive administrationCandidate vaccinesHeN miceFatal encephalitisImmunization experimentsImmunoblot assayWN virusInfectionVirus isolatesEnvelope proteinProtein antiserumVirusMiceEncephalitisPatientsImmunization