2017
Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus
Nunes MRT, Contreras-Gutierrez MA, Guzman H, Martins LC, Barbirato MF, Savit C, Balta V, Uribe S, Vivero R, Suaza JD, Oliveira H, Neto J, Carvalho VL, da Silva SP, Cardoso JF, de Oliveira RS, da Silva Lemos P, Wood TG, Widen SG, Vasconcelos PFC, Fish D, Vasilakis N, Tesh RB. Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus. Virology 2017, 504: 152-167. PMID: 28193550, PMCID: PMC5394984, DOI: 10.1016/j.virol.2017.01.022.Peer-Reviewed Original ResearchConceptsInsect-specific virusesGenetic characterizationNovel insect-specific virusesPlant virus generaMajor phylogenetic groupsHigh genetic variabilityComprehensive genetic characterizationFull-length sequencesCross-species transmissionPhylogenetic relationshipsEvolutionary analysisVirus taxaGenetic variabilityPhylogenetic groupsDistinct generaVirus genusNegevirusesMolecular analysisGenusMolecular epidemiologyDiverse groupPhlebotomine sandfliesTaxaArthropodsVirus
2008
MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi
Margos G, Gatewood A, Aanensen D, Hanincová K, Terekhova D, Vollmer S, Cornet M, Piesman J, Donaghy M, Bormane A, Hurn M, Feil E, Fish D, Casjens S, Wormser G, Schwartz I, Kurtenbach K. MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 8730-8735. PMID: 18574151, PMCID: PMC2435589, DOI: 10.1073/pnas.0800323105.Peer-Reviewed Original ResearchConceptsHousekeeping genesIntergenic spacerPopulation structureGeographic population structureChromosomal housekeeping genesMultilocus sequence typing (MLST) schemeB. burgdorferiPhylogenetic signalNorth AmericaEvolutionary relationshipsSequence typing schemeEvolutionary trajectoriesIGS locusSequence dataMLST schemeCultured isolatesGenesBorrelia burgdorferiOuter surface protein CMLST dataDistinct populationsSurface protein CBacterium Borrelia burgdorferiEuropean populationsVector-borne diseases
2004
Typing of Borrelia Relapsing Fever Group Strains - Volume 10, Number 9—September 2004 - Emerging Infectious Diseases journal - CDC
Bunikis J, Tsao J, Garpmo U, Berglund J, Fish D, Barbour A. Typing of Borrelia Relapsing Fever Group Strains - Volume 10, Number 9—September 2004 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2004, 10: 1661-1664. PMID: 15498172, PMCID: PMC3320305, DOI: 10.3201/eid1009.040236.Peer-Reviewed Original ResearchSequence typing reveals extensive strain diversity of the Lyme borreliosis agents Borrelia burgdorferi in North America and Borrelia afzelii in Europe
Bunikis J, Garpmo U, Tsao J, Berglund J, Fish D, Barbour A. Sequence typing reveals extensive strain diversity of the Lyme borreliosis agents Borrelia burgdorferi in North America and Borrelia afzelii in Europe. Microbiology 2004, 150: 1741-1755. PMID: 15184561, DOI: 10.1099/mic.0.26944-0.Peer-Reviewed Original ResearchConceptsIntergenic spacerGenetic diversityIGS locusIGS genotypesOspC allelesHigh nucleotide diversityDistinct clonal lineagesGreater genetic diversityB. burgdorferiNorth-eastern United StatesTick vector Ixodes scapularisNucleotide diversityMonophyletic lineageSignature nucleotidesPhylogenetic studiesLinkage groupsIGS sequencesPhylogenetic inferenceIntragenic recombinationExtensive strain diversityDetectable recombinationVector Ixodes scapularisClonal lineagesField sitesLoci
2003
Genetic Variability within Borrelia burgdorferi Sensu Lato Genospecies Established by PCR-Single-Strand Conformation Polymorphism Analysis of the rrfA-rrlB Intergenic Spacer in Ixodes ricinus Ticks from the Czech Republic
Derdáková M, Beati L, Pet'ko B, Stanko M, Fish D. Genetic Variability within Borrelia burgdorferi Sensu Lato Genospecies Established by PCR-Single-Strand Conformation Polymorphism Analysis of the rrfA-rrlB Intergenic Spacer in Ixodes ricinus Ticks from the Czech Republic. Applied And Environmental Microbiology 2003, 69: 509-516. PMID: 12514035, PMCID: PMC152394, DOI: 10.1128/aem.69.1.509-516.2003.Peer-Reviewed Original ResearchAnimalsBacterial Typing TechniquesBorrelia burgdorferi GroupCzech RepublicDNA, BacterialDNA, Ribosomal SpacerGenetic VariationGenotypeHumansIxodesMolecular Sequence DataPhylogenyPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthPolymorphism, Single-Stranded ConformationalSequence Analysis, DNA