1992
Bacteriorhodopsin can be refolded from two independently stable transmembrane helices and the complementary five-helix fragment.
Kahn T, Engelman D. Bacteriorhodopsin can be refolded from two independently stable transmembrane helices and the complementary five-helix fragment. Biochemistry 1992, 31: 6144-51. PMID: 1627558, DOI: 10.1021/bi00141a027.Peer-Reviewed Original ResearchConceptsStable transmembrane helixSecond helical segmentX-ray diffractionCovalent connectionAbsorption spectroscopyTwo-dimensional crystalsIndependent folding domainsBacteriorhodopsinHelical segmentsNative structureHelixSpectroscopyPeptidesDiffractionTransmembrane helicesMoleculesCrystalsFragmentsMaterialsStructure
1967
Characterization of the plasma membrane of Mycoplasma laidlawii. I. Sodium dodecyl sulfate solubilization
Engelman D, Terry T, Morowitz H. Characterization of the plasma membrane of Mycoplasma laidlawii. I. Sodium dodecyl sulfate solubilization. Biochimica Et Biophysica Acta 1967, 135: 381-390. PMID: 6048810, DOI: 10.1016/0005-2736(67)90028-4.Peer-Reviewed Original ResearchConceptsSodium dodecyl sulfate solubilizationProtein-detergent complexesSeparate lipidsDetergent solubilizationAnalytical ultracentrifugationPlasma membraneLipoprotein subunitsSchlieren peakSchlieren patternsSolubilizationDistribution of proteinsPreparationSolubilized membrane preparationsMembrane proteinsMembraneIntermediatesMycoplasma laidlawiiDensity gradient sedimentationComplexesMembrane preparationsProteinCharacterizationLipidsPropertiesUltracentrifugation