1995
Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *
Günzl A, Tschudi C, Nakaar V, Ullu E. Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *. Journal Of Biological Chemistry 1995, 270: 17287-17291. PMID: 7542235, DOI: 10.1074/jbc.270.29.17287.Peer-Reviewed Original ResearchConceptsIntragenic control regionRNA polymerase IIIControl regionPolymerase IIIU2 small nuclear RNA genesTranscription systemSmall nuclear RNA genesGene expressionInsect form trypanosomesNuclear RNA genesU2 snRNA genesSnRNA gene promotersB-box elementsFull transcriptional activitySeries of mutationsSnRNA genesRNA genesInsect formsRNA polymeraseGene promoterTrypanosoma bruceiCell-free extractsTransient expressionBox elementTranscriptional activity
1994
Upstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes
Nakaar V, Dare A, Hong D, Ullu E, Tschudi C. Upstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes. Molecular And Cellular Biology 1994, 14: 6736-6742. DOI: 10.1128/mcb.14.10.6736-6742.1994.Peer-Reviewed Original ResearchSmall RNA genesTRNA genesRNA genesSmall nuclear RNARegulatory elementsU6 small nuclear RNAUpstream tRNA genesIntragenic promoter elementsT. brucei cellsU6 snRNA genesRNA polymerase IIIB-box elementsSnRNA lociGenome organizationSnRNA genesRNA lociSmall RNAsBrucei cellsB-boxSubstitution mutantsPolymerase IIIFamily TrypanosomatidaeNuclear RNAGene transcriptionPromoter elementsUpstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes.
Nakaar V, Dare A, Hong D, Ullu E, Tschudi C. Upstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes. Molecular And Cellular Biology 1994, 14: 6736-6742. PMID: 7523857, PMCID: PMC359204, DOI: 10.1128/mcb.14.10.6736.Peer-Reviewed Original ResearchConceptsSmall RNA genesTRNA genesRNA genesSmall nuclear RNARegulatory elementsU6 small nuclear RNAUpstream tRNA genesIntragenic promoter elementsT. brucei cellsU6 snRNA genesRNA polymerase IIIB-box elementsSnRNA lociGenome organizationSnRNA genesRNA lociSmall RNAsBrucei cellsB-boxSubstitution mutantsPolymerase IIIFamily TrypanosomatidaeNuclear RNAGene transcriptionPromoter elementsRNA Polymerase III-Mediated Transcription of the Trypanosome U2 Small Nuclear RNA Gene Is Controlled by both Intragenic and Extragenic Regulatory Elements
Fantoni A, Dare A, Tschudi C. RNA Polymerase III-Mediated Transcription of the Trypanosome U2 Small Nuclear RNA Gene Is Controlled by both Intragenic and Extragenic Regulatory Elements. Molecular And Cellular Biology 1994, 14: 2021-2028. DOI: 10.1128/mcb.14.3.2021-2028.1994.Peer-Reviewed Original ResearchU2 snRNA genesSnRNA genesU2 small nuclear RNA genesSmall nuclear RNA genesRNA polymerase III transcriptionRNA polymerase III-mediated transcriptionCis-acting regulatory sequencesPolymerase III transcriptionRNA polymerase IIIII-mediated transcriptionInternal control regionInsect form trypanosomesTranscription start siteTrimethyl cap structureTRNA genesIII transcriptionPolymerase IIU2 snRNARNA genesStart sitePromoter elementsRegulatory sequencesCoding regionElement transcriptionIntragenic sequencesRNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements.
Fantoni A, Dare A, Tschudi C. RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements. Molecular And Cellular Biology 1994, 14: 2021-2028. PMID: 8114733, PMCID: PMC358562, DOI: 10.1128/mcb.14.3.2021.Peer-Reviewed Original ResearchMeSH KeywordsAmanitinsAnimalsBase SequenceConsensus SequenceDNA, ProtozoanGenes, ProtozoanMolecular Sequence DataPromoter Regions, GeneticRegulatory Sequences, Nucleic AcidRNA Polymerase IIIRNA, Small NuclearSequence AlignmentSequence DeletionSequence Homology, Nucleic AcidTranscription, GeneticTrypanosomaConceptsU2 small nuclear RNA genesSmall nuclear RNA genesNuclear RNA genesU2 snRNA genesSnRNA genesRNA genesCis-acting regulatory sequencesRNA polymerase III transcriptsInsect form trypanosomesSnRNA gene expressionRNA polymerase IIInternal control regionRNA polymerase IIIPolymerase III transcriptsTranscription start siteIII-like enzymeTRNA genesPolymerase IIRNA polymeraseRegulatory sequencesU2 snRNAControl regionPolymerase IIIStart siteRegulatory elementsRNA Polymerase III-Mediated Transcription of the Trypanosome U2 Small Nuclear RNA Gene Is Controlled by both Intragenic and Extragenic Regulatory Elements
Fantoni A, Dare A, Tschudi C. RNA Polymerase III-Mediated Transcription of the Trypanosome U2 Small Nuclear RNA Gene Is Controlled by both Intragenic and Extragenic Regulatory Elements. Molecular And Cellular Biology 1994, 14: 2021-2028. DOI: 10.1128/mcb.14.3.2021-2028.1994.Peer-Reviewed Original ResearchU2 small nuclear RNA genesSmall nuclear RNA genesNuclear RNA genesU2 snRNA genesSnRNA genesRNA genesRNA polymeraseCis-acting regulatory sequencesRNA polymerase III transcriptsInsect form trypanosomesSnRNA gene expressionRNA polymerase IIInternal control regionPolymerase III transcriptsTranscription start siteIII-like enzymeTRNA genesPolymerase IIRegulatory sequencesU2 snRNAControl regionStart siteRegulatory elementsPromoter elementsMutant constructs