2000
Determinants for cap trimethylation of the U2 small nuclear RNA are not conserved between Trypanosoma brucei and higher eukaryotic organisms
Günzl A, Bindereif A, Ullu E, Tschudi C. Determinants for cap trimethylation of the U2 small nuclear RNA are not conserved between Trypanosoma brucei and higher eukaryotic organisms. Nucleic Acids Research 2000, 28: 3702-3709. PMID: 11000261, PMCID: PMC110770, DOI: 10.1093/nar/28.19.3702.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBinding SitesCell LineConserved SequenceGuanosineMethylationMolecular Sequence DataMutationNucleic Acid ConformationPrecipitin TestsPromoter Regions, GeneticProtein BindingRegulatory Sequences, Nucleic AcidRibonucleoproteins, Small NuclearRNA CapsRNA, ProtozoanRNA, Small NuclearTransfectionTrypanosoma brucei bruceiConceptsCap trimethylationRibonucleoprotein particleEukaryotic organismsU2 snRNATrypanosoma bruceiU2 small nuclear RNA genesCap structureProtist parasite Trypanosoma bruceiSmall nuclear RNA genesCommon proteinsU2 small nuclear RNANuclear RNA genesHigher eukaryotic organismsRNA polymerase IIRNA polymerase IIISmall nuclear RNAMutant U2 snRNAsSet of proteinsParasite Trypanosoma bruceiSpliceosomal snRNPsRNP assemblyNuclear importPolymerase IIRNA genesSequence motifs
1997
Structure of the Trypanosoma brucei U6 snRNA gene promoter
Nakaar V, Günzl A, Ullu E, Tschudi C. Structure of the Trypanosoma brucei U6 snRNA gene promoter. Molecular And Biochemical Parasitology 1997, 88: 13-23. PMID: 9274863, DOI: 10.1016/s0166-6851(97)00078-9.Peer-Reviewed Original ResearchConceptsU6 snRNA gene promoterSnRNA gene promotersB-boxGene promoterA boxSnRNA gene transcriptionInternal control regionRNA polymerase IIITRNA-like genesU6 transcriptionChromatin organizationTranscription extractRNA genesTranscription unitYeast SaccharomycesYeast systemControl regionPolymerase IIIStart siteGene transcriptionNucleotides upstreamPromoter elementsTrypanosoma bruceiIntragenic sequencesSaturation mutagenesis
1995
Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *
Günzl A, Tschudi C, Nakaar V, Ullu E. Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *. Journal Of Biological Chemistry 1995, 270: 17287-17291. PMID: 7542235, DOI: 10.1074/jbc.270.29.17287.Peer-Reviewed Original ResearchConceptsIntragenic control regionRNA polymerase IIIControl regionPolymerase IIIU2 small nuclear RNA genesTranscription systemSmall nuclear RNA genesGene expressionInsect form trypanosomesNuclear RNA genesU2 snRNA genesSnRNA gene promotersB-box elementsFull transcriptional activitySeries of mutationsSnRNA genesRNA genesInsect formsRNA polymeraseGene promoterTrypanosoma bruceiCell-free extractsTransient expressionBox elementTranscriptional activity
1994
Upstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes
Nakaar V, Dare A, Hong D, Ullu E, Tschudi C. Upstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes. Molecular And Cellular Biology 1994, 14: 6736-6742. DOI: 10.1128/mcb.14.10.6736-6742.1994.Peer-Reviewed Original ResearchSmall RNA genesTRNA genesRNA genesSmall nuclear RNARegulatory elementsU6 small nuclear RNAUpstream tRNA genesIntragenic promoter elementsT. brucei cellsU6 snRNA genesRNA polymerase IIIB-box elementsSnRNA lociGenome organizationSnRNA genesRNA lociSmall RNAsBrucei cellsB-boxSubstitution mutantsPolymerase IIIFamily TrypanosomatidaeNuclear RNAGene transcriptionPromoter elementsUpstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes.
Nakaar V, Dare A, Hong D, Ullu E, Tschudi C. Upstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes. Molecular And Cellular Biology 1994, 14: 6736-6742. PMID: 7523857, PMCID: PMC359204, DOI: 10.1128/mcb.14.10.6736.Peer-Reviewed Original ResearchConceptsSmall RNA genesTRNA genesRNA genesSmall nuclear RNARegulatory elementsU6 small nuclear RNAUpstream tRNA genesIntragenic promoter elementsT. brucei cellsU6 snRNA genesRNA polymerase IIIB-box elementsSnRNA lociGenome organizationSnRNA genesRNA lociSmall RNAsBrucei cellsB-boxSubstitution mutantsPolymerase IIIFamily TrypanosomatidaeNuclear RNAGene transcriptionPromoter elementsRNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements.
Fantoni A, Dare A, Tschudi C. RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements. Molecular And Cellular Biology 1994, 14: 2021-2028. PMID: 8114733, PMCID: PMC358562, DOI: 10.1128/mcb.14.3.2021.Peer-Reviewed Original ResearchMeSH KeywordsAmanitinsAnimalsBase SequenceConsensus SequenceDNA, ProtozoanGenes, ProtozoanMolecular Sequence DataPromoter Regions, GeneticRegulatory Sequences, Nucleic AcidRNA Polymerase IIIRNA, Small NuclearSequence AlignmentSequence DeletionSequence Homology, Nucleic AcidTranscription, GeneticTrypanosomaConceptsU2 small nuclear RNA genesSmall nuclear RNA genesNuclear RNA genesU2 snRNA genesSnRNA genesRNA genesCis-acting regulatory sequencesRNA polymerase III transcriptsInsect form trypanosomesSnRNA gene expressionRNA polymerase IIInternal control regionRNA polymerase IIIPolymerase III transcriptsTranscription start siteIII-like enzymeTRNA genesPolymerase IIRNA polymeraseRegulatory sequencesU2 snRNAControl regionPolymerase IIIStart siteRegulatory elements