2000
Cotranscriptional Cap 4 Formation on the Trypanosoma brucei Spliced Leader RNA*
Mair G, Ullu E, Tschudi C. Cotranscriptional Cap 4 Formation on the Trypanosoma brucei Spliced Leader RNA*. Journal Of Biological Chemistry 2000, 275: 28994-28999. PMID: 10880518, DOI: 10.1074/jbc.m004193200.Peer-Reviewed Original ResearchConceptsCap 4 structureSL RNAMRNA cap formationT. brucei cellsSpliced leader RNARNA cap structureSpliced leader sequenceTranscriptional arrestTrypanosomatid protozoaBrucei cellsLeader RNACap 4Cotranscriptional mechanismLeader sequenceTrypanosoma bruceiCap structureRNA transcriptsRibonucleoprotein formationAdenosine residuesPosition 117RNATranscriptsCap formationNucleotidesEukaryotes
1998
Trypanosome Capping Enzymes Display a Novel Two-Domain Structure
Silva E, Ullu E, Kobayashi R, Tschudi C. Trypanosome Capping Enzymes Display a Novel Two-Domain Structure. Molecular And Cellular Biology 1998, 18: 4612-4619. PMID: 9671471, PMCID: PMC109047, DOI: 10.1128/mcb.18.8.4612.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCloning, MolecularConserved SequenceCrithidia fasciculataDNA, ProtozoanGuanosine MonophosphateHumansMiceMolecular Sequence DataRecombinant Fusion ProteinsRNA NucleotidyltransferasesSequence Homology, Amino AcidTrypanosoma brucei bruceiConceptsSpliceosomal small nuclear RNAsM7G capCapping enzymeTwo-domain structureG capYeast capping enzymesSmall nuclear RNAPhosphate-binding loopAmino-terminal halfCarboxy-terminal halfGTP-binding proteinsDiphosphate endTrans splicingSignature motifTranscript elongationM7G residueSL sequenceTrypanosomatid protozoaEukaryotic mRNAsLeader RNAGene codesRNA modificationsNuclear RNAOverall identityTrypanosoma brucei
1996
Trans -splicing in trypanosotnatid protozoa
Ullu E, Tschudi C, Gunzl A. Trans -splicing in trypanosotnatid protozoa. 1996, 115-133. DOI: 10.1093/oso/9780199636020.003.0007.Peer-Reviewed Original ResearchTrans splicingSplice siteT. brucei cellsPolycistronic pre-mRNARNA processing reactionsEnd of transcriptsDifferent variant surface glycoproteinsSeparate RNA moleculesVariant surface glycoproteinSL RNAList of organismsSmall RNAsBrucei cellsSL sequenceTrypanosomatid protozoaMature mRNALeader RNAMature speciesPre-mRNATrypanosoma bruceiRNA moleculesProcessing reactionsRNASurface glycoproteinFirst evidence
1993
Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts.
Ullu E, Matthews K, Tschudi C. Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts. Molecular And Cellular Biology 1993, 13: 720-725. PMID: 8417363, PMCID: PMC358950, DOI: 10.1128/mcb.13.1.720.Peer-Reviewed Original ResearchConceptsRNA-processing reactionsTrans splicingPre-mRNASplice sitePolycistronic transcription unitsSplice acceptor siteTrypanosome genesMRNA biogenesisTranscription unitTrypanosome mRNAsLeader RNATubulin transcriptsSplicingAlpha-tubulinPolyadenylationMRNA synthesisAcceptor sitesTrypanosomesTemporal orderMRNABiogenesisGenesTranscriptsRNAOrganismsTemporal Order of RNA-Processing Reactions in Trypanosomes: Rapid trans Splicing Precedes Polyadenylation of Newly Synthesized Tubulin Transcripts
Ullu E, Matthews K, Tschudi C. Temporal Order of RNA-Processing Reactions in Trypanosomes: Rapid trans Splicing Precedes Polyadenylation of Newly Synthesized Tubulin Transcripts. Molecular And Cellular Biology 1993, 13: 720-725. DOI: 10.1128/mcb.13.1.720-725.1993.Peer-Reviewed Original ResearchRNA-processing reactionsTrans splicingPre-mRNASplice sitePolycistronic transcription unitsRNA processing reactionsSplice acceptor siteTrypanosome genesMRNA biogenesisTranscription unitTrypanosome mRNAsLeader RNATubulin transcriptsSplicingPolyadenylationΒ-tubulinΑ-tubulinMRNA synthesisAcceptor sitesTrypanosomesTemporal orderMRNABiogenesis
1991
Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA.
Ullu E, Tschudi C. Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1991, 88: 10074-10078. PMID: 1719544, PMCID: PMC52870, DOI: 10.1073/pnas.88.22.10074.Peer-Reviewed Original Research
1990
Permeable trypanosome cells as a model system for transceiption and trans-splicing
Ullu E, Tschudi C. Permeable trypanosome cells as a model system for transceiption and trans-splicing. Nucleic Acids Research 1990, 18: 3319-3326. PMID: 2356121, PMCID: PMC330939, DOI: 10.1093/nar/18.11.3319.Peer-Reviewed Original ResearchConceptsAlpha-tubulin RNASL RNAFree linear moleculesHigh molecular weight RNAMolecular weight RNARNA speciesLeader RNATrypanosome cellsDifferent genesWeight RNARNATranscriptionModel systemCritical rolePermeable cellsConcentrations of monovalentIntronsCellsGenesLesser extentHigh potassium concentrationSpeciesTrypanosomaMgCl2 concentrationLinear moleculesDestruction of U2, U4, or U6 small nuclear RNA blocks Trans splicing in trypanosome cells
Tschudi C, Ullu E. Destruction of U2, U4, or U6 small nuclear RNA blocks Trans splicing in trypanosome cells. Cell 1990, 61: 459-466. PMID: 1692263, DOI: 10.1016/0092-8674(90)90527-l.Peer-Reviewed Original ResearchConceptsTrans splicingU snRNAsPre-mRNAU6 small nuclear RNAU4/U6 snRNPsAlpha-tubulin transcriptsSmall nuclear RNAHigh molecular weight RNAMolecular weight RNASite-directed cleavageSL sequenceLeader RNATrypanosome cellsU6 snRNPsNuclear RNAU6 snRNATrypanosoma bruceiSplicing processSplicingWeight RNASnRNAEarly stepsRNAase HRNAIntrons
1986
The U2 RNA analogue of Trypanosoma brucei gambiense: implications for a splicing mechanism in trypanosomes
Tschudi C, Richards F, Ullu E. The U2 RNA analogue of Trypanosoma brucei gambiense: implications for a splicing mechanism in trypanosomes. Nucleic Acids Research 1986, 14: 8893-8903. PMID: 3786140, PMCID: PMC311918, DOI: 10.1093/nar/14.22.8893.Peer-Reviewed Original ResearchConceptsU2 RNASpliced leader RNASingle-copy geneSignificant sequence homologyMaturation of mRNAMammalian counterpartsCopy geneU RNALeader RNASplicing mechanismSequence homologySecondary structureRNATrypanosomesGenesRNA analogSplicingHomologyTrypanosoma brucei gambiensePossible interactionsMRNABrucei gambienseMaturation