1989
The influence of perfusate viscosity, RBC deformability and drag on the function of an isolated perfused rat kidney
Sumpio B, Upchurch G, Johnson G. The influence of perfusate viscosity, RBC deformability and drag on the function of an isolated perfused rat kidney. Journal Of Surgical Research 1989, 46: 4-8. PMID: 2915534, DOI: 10.1016/0022-4804(89)90174-1.Peer-Reviewed Original ResearchConceptsRenal functionMean renal arterial pressureRat kidneyKrebs-HCO3 bufferRenal arterial pressureReabsorption of sodiumArterial pressureUrine flowOrgan functionClinical interestHCO3- bufferPerfusate solutionHematocritBlood rheologyFunctional parametersPerfusate viscosityRBC deformabilityKidneyPerfusateDegrees CRBCsDrag-reducing agentsGFRCreatinineAgents
1987
Mitochondrial Respiratory and Calcium Studies
SUMPIO B, BAUE A, CHAUDRY I. Mitochondrial Respiratory and Calcium Studies. Annals Of Surgery 1987, 206: 655-660. PMID: 3499878, PMCID: PMC1493310, DOI: 10.1097/00000658-198711000-00017.Peer-Reviewed Original ResearchConceptsRespiratory control ratioVerapamil pretreatmentTotal tissue Ca2Tissue ATP levelsCyA-induced nephrotoxicityCyA treatmentPharmacologic regimenRenal functionMitochondrial respiratory functionSimultaneous administrationCalcium studiesRespiratory functionKrebs bufferCortical mitochondriaMumol/Tissue Ca2Rat kidneyADP/O ratioCyANephrotoxicityATP levelsControl ratioVerapamilMitochondrial respiratoryCa2
1986
Effects of ATP-MgCl2 and adenosine-MgCl2 administration on intracellular ATP levels in the kidney
Sumpio B, Hull M, Bauer A, Chaudry I. Effects of ATP-MgCl2 and adenosine-MgCl2 administration on intracellular ATP levels in the kidney. Biochimica Et Biophysica Acta 1986, 862: 303-308. PMID: 3778894, DOI: 10.1016/0005-2736(86)90232-4.Peer-Reviewed Original ResearchConceptsATP-MgCl2 administrationColloid oncotic pressureIntracellular ATPATP-MgCl2Exogenous administrationIntracellular ATP levelsRat kidneyOncotic pressureAdministrationTotal intracellular ATPPerfusate solutionKidneyIntracellular accumulationATP levelsAdenine nucleotidesSignificant accumulationATPPerfusate
1985
Accelerated functional recovery of isolated rat kidney with ATP-MgCl2 after warm ischemia
Sumpio B, Chaudry I, Clemens M, Baue A. Accelerated functional recovery of isolated rat kidney with ATP-MgCl2 after warm ischemia. AJP Regulatory Integrative And Comparative Physiology 1985, 248: r752-r752. DOI: 10.1152/ajpregu.1985.248.6.r752-r.Peer-Reviewed Original ResearchReduction of the drug-induced nephrotoxicity by ATP-MgCl2. 1. Effects on the cis-Diamminedichloroplatinum-treated isolated perfused kidneys
Sumpio B, Chaudry I, Baue A. Reduction of the drug-induced nephrotoxicity by ATP-MgCl2. 1. Effects on the cis-Diamminedichloroplatinum-treated isolated perfused kidneys. Journal Of Surgical Research 1985, 38: 429-437. PMID: 3872963, DOI: 10.1016/0022-4804(85)90058-7.Peer-Reviewed Original ResearchConceptsAcute renal failureATP-MgCl2Hr of perfusionCis-DiamminedichloroplatinumNephrotoxic effectsReabsorptive capacityCDDP treatmentRat kidneyATP-MgCl2 treatmentIsolated rat kidneyDrug-induced nephrotoxicityDirect nephrotoxic effectNew therapeutic modalitiesProximal tubule cellsCancer chemotherapeutic agentsCDDP therapyRenal failureMarked diuresisProtein reabsorptionTherapeutic modalitiesTubule cellsChemotherapeutic agentsMicrograms/KidneyMarked inhibitionAdenosine Triphosphate—Magnesium Chloride Ameliorates Reperfusion Injury Following Ischemia as Determined by Phosphorus Nuclear Magnetic Resonance
Sumpio B, Chaudry I, Baue A. Adenosine Triphosphate—Magnesium Chloride Ameliorates Reperfusion Injury Following Ischemia as Determined by Phosphorus Nuclear Magnetic Resonance. JAMA Surgery 1985, 120: 233-240. PMID: 3872113, DOI: 10.1001/archsurg.1985.01390260091013.Peer-Reviewed Original ResearchConceptsIntracellular acidosisPerfusate flowATP levelsAdenosine triphosphate-magnesium chlorideRenal ATP levelsMinutes of reperfusionTissue ATP levelsEffects of ischemiaAdenosine triphosphateML/minPostischemic administrationReperfusion injuryRenal ischemiaWarm ischemiaATP-MgCl2ReperfusionIschemiaInorganic phosphate peakIntracellular adenosine triphosphatePi peakRat kidneyAcidosisATP depletionMinutesMagnetic resonance
1984
Accelerated functional recovery of isolated rat kidney with ATP-MgCl2 after warm ischemia
Sumpio BE, Chaudry IH, Clemens MG, Baue AE. Accelerated functional recovery of isolated rat kidney with ATP-MgCl2 after warm ischemia. American Journal Of Physiology 1984, 247: r1047-r1053. PMID: 6334446, DOI: 10.1152/ajpregu.1984.247.6.r1047.Peer-Reviewed Original ResearchConceptsRenal perfusate flow rateMM ATP-MgCl2Min of perfusionIsolated rat kidneyATP-MgCl2Renal functionWarm ischemiaWhole kidney inulin clearanceRat kidneyAccelerated functional recoveryMin of reperfusionMin of ischemiaUrine flow rateTissue ATP levelsFractional absorptionFunctional recoveryNormothermic ischemiaIschemia resultsMin ischemiaPerfusate flow rateInulin clearanceEarly recoverySystemic effectsIschemiaSodium valuesRenal hydrolysis of absorbed protein: influence of load and lysosomal pH
Camargo MJ, Sumpio BE, Maack T. Renal hydrolysis of absorbed protein: influence of load and lysosomal pH. American Journal Of Physiology 1984, 247: f656-f664. PMID: 6093557, DOI: 10.1152/ajprenal.1984.247.4.f656.Peer-Reviewed Original ResearchConceptsNormal filtered loadEfflux of radioactivityRenal tubular cellsEffect of alkalinizationProtein absorption dropletsRenal catabolismNormal lysosomal functionLysosomotropic weak basesFiltered loadTubular cellsKidney 1Abnormal depositionRat kidneyKidneyAbsorption dropletsSaturable processIntracellular hydrolysisSaturable functionLysosomal functionPerfusateLysosomal pHLow uptake rateSephadex chromatographyReversible mannerHalf times
1982
Kinetics, competition, and selectivity of tubular absorption of proteins
Sumpio BE, Maack T. Kinetics, competition, and selectivity of tubular absorption of proteins. American Journal Of Physiology 1982, 243: f379-f392. PMID: 7124951, DOI: 10.1152/ajprenal.1982.243.4.f379.Peer-Reviewed Original ResearchConceptsBeta 2MTubular absorptionHuman growth hormoneLow perfusate calcium concentrationsPerfusate calcium concentrationMorphologic damageTubular epitheliumProximal tubulesGrowth hormoneRat kidneyCationic proteinCalcium concentrationSaturable processSaturating loadSelective effectLuminal wallLow apparent affinityTubulesImportant determinantReversible mannerApparent affinityMicrovilliProteinKidney