2008
The Effect of Different Frequencies of Stretch on Human Dermal Keratinocyte Proliferation and Survival
Nishimura K, Blume P, Ohgi S, Sumpio BE. The Effect of Different Frequencies of Stretch on Human Dermal Keratinocyte Proliferation and Survival. Journal Of Surgical Research 2008, 155: 125-131. PMID: 19059608, DOI: 10.1016/j.jss.2008.07.029.Peer-Reviewed Original ResearchConceptsERK 1/2P38 MAPKProtein kinaseKeratinocyte proliferation rateCell deathAkt inhibitorCyclic stretchDNA synthesisAktTransferase-mediated dUTP nick-end labeling stainingRepetitive stretchApoptosisMAPKDUTP nick end labeling stainingProliferation rateHuman dermalCell numberNick end labeling stainingTUNEL-positive cellsDermal keratinocytesStretchTerminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stainingBrdU stainingKeratinocyte proliferationProliferation
2006
Role of AKT in cyclic strain-induced endothelial cell proliferation and survival
Nishimura K, Li W, Hoshino Y, Kadohama T, Asada H, Ohgi S, Sumpio BE. Role of AKT in cyclic strain-induced endothelial cell proliferation and survival. American Journal Of Physiology - Cell Physiology 2006, 290: c812-c821. PMID: 16469863, DOI: 10.1152/ajpcell.00347.2005.Peer-Reviewed Original ResearchConceptsEndothelial cellsCycles/minRole of AktEC proliferationCultured bovine aortic endothelial cellsSurvival of ECsAortic endothelial cellsApoptotic endothelial cellsGSK-3betaBovine aortic endothelial cellsEndothelial cell proliferationFrequency of strainsPositive cellsGlycogen synthase kinaseKinase-dead AktCell proliferationCaspase-3AktSurvivalCell numberSynthase kinaseProliferationEarly phosphorylationBad phosphorylation
2004
Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway
Li W, Sumpio BE. Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway. AJP Heart And Circulatory Physiology 2004, 288: h1591-h1597. PMID: 15591103, DOI: 10.1152/ajpheart.00382.2004.Peer-Reviewed Original ResearchMeSH Keywords3-Phosphoinositide-Dependent Protein KinasesAndrostadienesAnimalsAntibiotics, AntineoplasticAortaCarrier ProteinsCattleCell DivisionCells, CulturedChromonesEndothelium, VascularEnzyme InhibitorsFlavonoidsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3MorpholinesPhosphatidylinositol 3-KinasesPhosphodiesterase InhibitorsPhosphoinositide-3 Kinase InhibitorsPhosphoproteinsPhosphorylationProtein KinasesProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktRibosomal Protein S6 KinasesSignal TransductionSirolimusTOR Serine-Threonine KinasesWortmanninConceptsVascular endothelial cell proliferationEndothelial cell proliferationStrain-induced activationSignal pathwayEC proliferationPD 98059Cell proliferationPI3K inhibitor wortmanninPI3K inhibitorsCycles/minExtracellular signal-regulated kinases 1Inhibitor PD 98059MTOR pathwaySignal-regulated kinases 1Bovine aortic ECsMammalian targetMTOR-4EK inhibitorsEukaryotic initiation factor 4EAortic ECsInitiation factor 4EMEK1 inhibitor PD 98059K activationProliferationRapamycin
2002
Photochemotherapy of vascular cells with 8‐methoxypsoralen and visible light: differential effects on endothelial and smooth muscle cells
Lee DM, Gasparro FP, Wang XJ, Kopec C, DeLeo K, Sumpio BE. Photochemotherapy of vascular cells with 8‐methoxypsoralen and visible light: differential effects on endothelial and smooth muscle cells. Photodermatology Photoimmunology & Photomedicine 2002, 18: 244-252. PMID: 12390666, DOI: 10.1034/j.1600-0781.2002.02770.x.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsEndothelial cellsPercutaneous transluminal coronary angioplastyMuscle cellsTransluminal coronary angioplastyLong-term efficacyAortic smooth muscle cellsBovine aortic smooth muscle cellsDose-dependent fashionReversible inhibitionCoronary angioplastyIntermediate doseLow doseHigh doseSMC proliferationCell countVascular cellsDoseProliferation of ECsEC proliferationDifferential effectsProliferationCellular migrationPresent studySignificant effect
1998
Induction of interleukin (IL)‐1α and β gene expression in human keratinocytes exposed to repetitive strain: Their role in strain‐induced keratinocyte proliferation and morphological change
Takei T, Kito H, Du W, Mills I, Sumpio B. Induction of interleukin (IL)‐1α and β gene expression in human keratinocytes exposed to repetitive strain: Their role in strain‐induced keratinocyte proliferation and morphological change. Journal Of Cellular Biochemistry 1998, 69: 95-103. PMID: 9548558, DOI: 10.1002/(sici)1097-4644(19980501)69:2<95::aid-jcb1>3.0.co;2-p.Peer-Reviewed Original ResearchConceptsIL-1 antibodyIL-1 alphaIL-1Keratinocyte proliferationIL-1 mRNA expressionKeratinocyte biologyIL-1 gene expressionInduction of interleukinIL-1 betaIL-1 expressionMorphological changesΒ gene expressionAutocrine regulationMRNA expressionBeta mRNAGene expressionBasal rateBasic cellular propertiesHuman keratinocytesAntibodiesKeratinocyte functionRepetitive strainNorthern blot analysisBlot analysisProliferation
1996
Protein phosphatase 2A in stretch‐induced endothelial cell proliferation
Murata K, Mills I, Sumpio B. Protein phosphatase 2A in stretch‐induced endothelial cell proliferation. Journal Of Cellular Biochemistry 1996, 63: 311-319. PMID: 8913882, DOI: 10.1002/(sici)1097-4644(19961201)63:3<311::aid-jcb6>3.0.co;2-#.Peer-Reviewed Original ResearchConceptsProtein phosphatase 2APhosphatase 2AProtein phosphatase 2A activityProtein phosphatase 1Phosphatase 2A activityProtein kinase CPhosphatase 1Bovine aortic endothelial cellsOkadaic acidGrowth suppressorEndothelial cellsKinase CEndothelial cell proliferationCell growthFetal bovine serumCellular proliferationAortic endothelial cellsCell proliferationCyclic strainBovine serumKey mechanismProliferationCellsStrainsSuppressor
1994
Inhibition of smooth muscle cell proliferation by visible light-activated psoralen.
Sumpio BE, Li G, Deckelbaum LI, Gasparro FP. Inhibition of smooth muscle cell proliferation by visible light-activated psoralen. Circulation Research 1994, 75: 208-213. PMID: 8033334, DOI: 10.1161/01.res.75.2.208.Peer-Reviewed Original ResearchIncreased ambient pressure stimulates proliferation and morphologic changes in cultured endothelial cells
Sumpio B, Widmann M, Ricotta J, Awolesi M, Watase M. Increased ambient pressure stimulates proliferation and morphologic changes in cultured endothelial cells. Journal Of Cellular Physiology 1994, 158: 133-139. PMID: 8263020, DOI: 10.1002/jcp.1041580117.Peer-Reviewed Original ResearchConceptsSystemic blood pressureCultured endothelial cellsBlood pressureSystemic pressureCell responsesEndothelial cell responsesBovine aortic ECsMorphologic changesEndothelial cellsAortic ECsRat type IEC phenotypeSignificant increaseCell numberEC proliferationVascular systemSignificant regulatory influenceCalf serumEndotheliumType IDigital monitorRegulatory influenceProliferationHg pressureCells
1993
Control of smooth muscle cell proliferation by psoralen photochemotherapy.
Sumpio B, Phan S, Gasparro F, Deckelbaum L. Control of smooth muscle cell proliferation by psoralen photochemotherapy. Journal Of Vascular Surgery 1993, 17: 1010-6; discussion 1016-8. PMID: 8505779, DOI: 10.1067/mva.1993.45747.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationSMC proliferationPsoralen photochemotherapyPeripheral vascular diseaseLong-term therapyCell proliferationInhibits SMC proliferationTrypan blue exclusionBypass graftDistal anastomosisBalloon angioplastyUVA photochemotherapyVascular diseaseIntimal hyperplasiaGrowth of SMCsBlue exclusionPhotochemotherapySMC viabilityTime courseProliferationUVADNA synthesisDaysAngioplastyControl of smooth muscle cell proliferation by psoralen photochemotherapy
Sumpio B, Phan S, Gasparro F, Deckelbaum L. Control of smooth muscle cell proliferation by psoralen photochemotherapy. Journal Of Vascular Surgery 1993, 17: 1010-1018. DOI: 10.1016/0741-5214(93)90671-8.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationSMC proliferationPsoralen photochemotherapyPeripheral vascular diseaseLong-term therapyCell proliferationInhibits SMC proliferationTrypan blue exclusionBypass graftDistal anastomosisBalloon angioplastyUVA photochemotherapyVascular diseaseIntimal hyperplasiaGrowth of SMCsBlue exclusionPhotochemotherapySMC viabilityTime courseProliferationUVADNA synthesisDaysAngioplasty
1992
Isolation and characterization of rabbit cardiac endothelial cells: Response to cyclic strain and growth factors in vitro
Widmann M, Letsou G, Phan S, Baldwin J, Sumpio B. Isolation and characterization of rabbit cardiac endothelial cells: Response to cyclic strain and growth factors in vitro. Journal Of Surgical Research 1992, 53: 331-334. PMID: 1405613, DOI: 10.1016/0022-4804(92)90057-7.Peer-Reviewed Original ResearchConceptsRetina-derived growth factorCardiac endothelial cellsEndothelial cellsGrowth factorPure endothelial cellsEC proliferationProliferative responseExogenous growth factorsTrophic effectsRabbit heartsAcetylated LDLCardiac endotheliumCollagenase digestionFetal bovine serumPulsatile strainSignificant increaseBovine serumProliferationEC morphologyCellsRegimenResponseLDLEndotheliumFactors