2016
Systemic and cell-specific mechanisms of vasculopathy induced by human immunodeficiency virus and highly active antiretroviral therapy
Haser GC, Sumpio B. Systemic and cell-specific mechanisms of vasculopathy induced by human immunodeficiency virus and highly active antiretroviral therapy. Journal Of Vascular Surgery 2016, 65: 849-859. PMID: 26994951, DOI: 10.1016/j.jvs.2016.01.036.Peer-Reviewed Original ResearchConceptsHuman immunodeficiency virusActive antiretroviral therapySmooth muscle cellsAntiretroviral therapyHIV patientsImmunodeficiency virusTraditional cardiovascular risk factorsMuscle cellsEndothelial cellsCardiovascular risk factorsChronic immune activationSerious cardiovascular eventsDevelopment of atherosclerosisVascular systemCardiovascular eventsVascular complicationsHIV medicationsChronic inflammationImmune activationMyocardial infarctionRisk factorsCell-specific mechanismsGeneral populationCholesterol metabolismCardiovascular pathology
2010
Thrombospondin 1, Fibronectin, and Vitronectin are Differentially Dependent Upon RAS, ERK1/2, and p38 for Induction of Vascular Smooth Muscle Cell Chemotaxis
Willis AI, Sadowitz B, Fuse S, Maier KG, Lee TS, Wang XJ, Tuszynski GP, Sumpio BE, Gahtan V. Thrombospondin 1, Fibronectin, and Vitronectin are Differentially Dependent Upon RAS, ERK1/2, and p38 for Induction of Vascular Smooth Muscle Cell Chemotaxis. Vascular And Endovascular Surgery 2010, 45: 55-62. PMID: 21193465, DOI: 10.1177/1538574410387677.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsCattleCells, CulturedChemotaxisFibronectinsHumansMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Muscle, Smooth, VascularMyocytes, Smooth MuscleP38 Mitogen-Activated Protein KinasesProtein Kinase InhibitorsRas ProteinsThrombospondin 1TransfectionVitronectinConceptsVascular smooth muscle cellsThrombospondin-1Smooth muscle cell chemotaxisFarnesyl protein transferase inhibitorSignal transduction pathwaysProtein transferase inhibitorsVascular smooth muscle cell chemotaxisBovine vascular smooth muscle cellsTSP-1Ras N17Transduction pathwaysSecond messenger systemsP38Smooth muscle cellsERK1/2VSMC migrationTransferase inhibitorsPD098059Cell chemotaxisSB202190Muscle cellsMessenger systemsVitronectinRAChemotaxisThe Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty
Basco MT, Yiu WK, Cheng SW, Sumpio BE. The Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty. Journal Of Vascular And Interventional Radiology 2010, 21: 910-915. PMID: 20417120, PMCID: PMC2878641, DOI: 10.1016/j.jvir.2010.02.016.Peer-Reviewed Original Research
2008
QS163. Differential Effects on Endothelial Cells and Smooth Muscle Cells After Freezing and Rewarming
Basco M, Yiu W, Sumpio B. QS163. Differential Effects on Endothelial Cells and Smooth Muscle Cells After Freezing and Rewarming. Journal Of Surgical Research 2008, 144: 332. DOI: 10.1016/j.jss.2007.12.405.Peer-Reviewed Original ResearchThrombospondin-1-Induced Migration Is Functionally Dependent Upon Focal Adhesion Kinase
Wang XJ, Maier K, Fuse S, Willis AI, Olson E, Nesselroth S, Sumpio BE, Gahtan V. Thrombospondin-1-Induced Migration Is Functionally Dependent Upon Focal Adhesion Kinase. Vascular And Endovascular Surgery 2008, 42: 256-262. PMID: 18319354, DOI: 10.1177/1538574408314440.Peer-Reviewed Original Research
2007
P139 Differential survival responses of vascular smooth muscle cells and endothelial cells towards different cycles of supercooling and rewarming in a model simulating cryoplasty
Yiu W, Cheng S, Sumpio B. P139 Differential survival responses of vascular smooth muscle cells and endothelial cells towards different cycles of supercooling and rewarming in a model simulating cryoplasty. Journal Of Surgical Research 2007, 137: 292. DOI: 10.1016/j.jss.2006.12.370.Peer-Reviewed Original Research
2005
In–Vitro Model for Evaluation of the Effects of Supercooling and Re-warming on Vascular Cells
Yiu W, Aruny J, Cheng S, Sumpio B. In–Vitro Model for Evaluation of the Effects of Supercooling and Re-warming on Vascular Cells. International Journal Of Angiology 2005, 14: 237-241. DOI: 10.1007/s00547-005-2055-5.Peer-Reviewed Original ResearchInitial technical success rateEndothelial cellsRe-warming phasePeripheral vascular diseaseTransluminal balloon angioplastyRate of restenosisTechnical success rateTUNEL-positive cellsSmooth muscle cellsBalloon angioplastyVascular diseaseEndothelial cell survivalMuscle cellsVascular cellsVitro modelVessel wallSuccess rateUnderlying mechanismRestenosisCell survivalCellsCellular responsesCritical roleAngioplastyHomocysteine promotes p38-dependent chemotaxis in bovine aortic smooth muscle cells
Akasaka K, Akasaka N, Di Luozzo G, Sasajima T, Sumpio BE. Homocysteine promotes p38-dependent chemotaxis in bovine aortic smooth muscle cells. Journal Of Vascular Surgery 2005, 41: 517-522. PMID: 15838488, DOI: 10.1016/j.jvs.2004.12.043.Peer-Reviewed Original ResearchConceptsEffect of homocysteineMigration of SMCsLevels of homocysteineProgressive intimal thickeningAortic smooth muscle cellsSmooth muscle cell migrationBovine aortic smooth muscle cellsPotential therapeutic implicationsSmooth muscle cellsP38 activationExposure of SMCMuscle cell migrationSMC chemotaxisRisk factorsSelective blockadeIntimal thickeningTherapeutic implicationsP38-dependent pathwaySMC proliferationBoyden chamberChemotactic potentialMuscle cellsHomocysteineFetal bovine serumP38 inhibitorIn–Vitro model for evaluation of the effects of supercooling and re-warming on vascular cells
Yiu W, Aruny J, Cheng S, Sumpio B. In–Vitro model for evaluation of the effects of supercooling and re-warming on vascular cells. International Journal Of Angiology 2005, 237-241. DOI: 10.1055/s-0031-1276516.Peer-Reviewed Original ResearchInitial technical success rateEndothelial cellsRe-warming phasePeripheral vascular diseaseTransluminal balloon angioplastyRate of restenosisTechnical success rateTUNEL-positive cellsSmooth muscle cellsBalloon angioplastyVascular diseaseEndothelial cell survivalMuscle cellsVascular cellsVitro modelVessel wallSuccess rateUnderlying mechanismRestenosisCell survivalCellsCellular responsesCritical roleAngioplasty
2003
Involvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells
Li W, Chen Q, Mills I, Sumpio BE. Involvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells. Journal Of Cellular Physiology 2003, 195: 202-209. PMID: 12652647, DOI: 10.1002/jcp.10230.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDNA-Binding ProteinsEnzyme InhibitorsImidazolesImmunosuppressive AgentsMAP Kinase Signaling SystemMitogen-Activated Protein KinasesMuscle, Smooth, VascularMyocytes, Smooth MuscleP38 Mitogen-Activated Protein KinasesPyridinesRegional Blood FlowRibosomal Protein S6 KinasesSirolimusStress, MechanicalTranscription FactorsConceptsAortic smooth muscle cellsBovine aortic smooth muscle cellsKinase pathwaySmooth muscle cell phenotypeSmooth muscle cellsStrain-induced proliferationBovine aortic SMCS6 kinaseMuscle cell phenotypeSpecific inhibitorProliferation of SMCsActivation of p38SMC proliferationAortic SMCsMuscle cellsSMC alignment
2002
Photochemotherapy of vascular cells with 8‐methoxypsoralen and visible light: differential effects on endothelial and smooth muscle cells
Lee DM, Gasparro FP, Wang XJ, Kopec C, DeLeo K, Sumpio BE. Photochemotherapy of vascular cells with 8‐methoxypsoralen and visible light: differential effects on endothelial and smooth muscle cells. Photodermatology Photoimmunology & Photomedicine 2002, 18: 244-252. PMID: 12390666, DOI: 10.1034/j.1600-0781.2002.02770.x.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsEndothelial cellsPercutaneous transluminal coronary angioplastyMuscle cellsTransluminal coronary angioplastyLong-term efficacyAortic smooth muscle cellsBovine aortic smooth muscle cellsDose-dependent fashionReversible inhibitionCoronary angioplastyIntermediate doseLow doseHigh doseSMC proliferationCell countVascular cellsDoseProliferation of ECsEC proliferationDifferential effectsProliferationCellular migrationPresent studySignificant effect
2001
Cyclic strain activates the pro-survival Akt protein kinase in bovine aortic smooth muscle cells
Chen A, Gortler D, Kilaru S, Araim O, Frangos S, Sumpio B. Cyclic strain activates the pro-survival Akt protein kinase in bovine aortic smooth muscle cells. Surgery 2001, 130: 378-381. PMID: 11490374, DOI: 10.1067/msy.2001.116668.Peer-Reviewed Original ResearchConceptsBovine aortic smooth muscle cellsAortic smooth muscle cellsSmooth muscle cellsProtein kinaseSerine/threonine protein kinaseThreonine protein kinasePro-survival functionAkt protein kinasePro-survival Akt kinaseMuscle cellsPhosphorylation of AktPlatelet-derived growth factorInhibition of apoptosisAkt kinasePrevalence of apoptosisArterial endothelial cellsWestern blot analysisCell survivalAkt phosphorylationKinaseBlot analysisApoptosisCyclic strainPhosphorylationArterial SMC proliferationThe C-terminal domain of thrombospondin-1 induces vascular smooth muscle cell chemotaxis
Nesselroth S, Willis A, Fuse S, Olson E, Lawler J, Sumpio B, Gahtan V. The C-terminal domain of thrombospondin-1 induces vascular smooth muscle cell chemotaxis. Journal Of Vascular Surgery 2001, 33: 595-600. PMID: 11241132, DOI: 10.1067/mva.2001.112318.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsVSMC chemotaxisThrombospondin-1VSMC migrationAortic vascular smooth muscle cellsTop wellsAcute phase reactantsBoyden chamber chemotaxisSmooth muscle cellsSmooth muscle cell chemotaxisVascular diseaseVascular lesionsVascular smooth muscle cell chemotaxisSerum-free mediumMuscle cellsTSP-1 moleculeCell chemotaxisChemotaxis assaysBottom wellsAntibodiesSelective inhibitionT-testCyclic Stretch Induces the Expression of Vascular Endothelial Growth Factor in Vascular Smooth Muscle Cells
Smith JD, Davies N, Willis AI, Sumpio BE, Zilla P. Cyclic Stretch Induces the Expression of Vascular Endothelial Growth Factor in Vascular Smooth Muscle Cells. Endothelium 2001, 8: 41-48. PMID: 11409850, DOI: 10.3109/10623320109063156.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicCattleCells, CulturedCoculture TechniquesCulture MediaEndothelial Growth FactorsEndothelium, VascularGene Expression RegulationLymphokinesMuscle, Smooth, VascularPhosphorylationReceptor Protein-Tyrosine KinasesReceptors, Growth FactorReceptors, Vascular Endothelial Growth FactorStress, MechanicalVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsVasodilationConceptsVascular smooth muscle cellsVascular endothelial growth factorSmooth muscle cellsEndothelial growth factorExposure of VSMCsMuscle cellsGrowth factorMigration of ECsBovine vascular smooth muscle cellsSteady-state levelsCyclic stretchNormal endothelial cell functionEndothelial cell functionVEGF receptor KDRRelease of VEGFVEGF receptor phosphorylationVEGF secretionMRNA steady-state levelsVEGF receptorsReceptor KDRVEGF mRNACell functionReceptor phosphorylationSignificant increaseEC survival
2000
Homocysteine stimulates MAP kinase in bovine aortic smooth muscle cells
Woo D, Dudrick S, Sumpio B. Homocysteine stimulates MAP kinase in bovine aortic smooth muscle cells. Surgery 2000, 128: 59-66. PMID: 10876187, DOI: 10.1067/msy.2000.106531.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDose-Response Relationship, DrugEnzyme ActivationEnzyme InhibitorsFlavonoidsHomocysteineMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMuscle, Smooth, VascularPhosphorylationConceptsMAP kinase phosphorylationMAP kinaseKinase phosphorylationBASMC proliferationBovine aortic smooth muscle cellsAortic smooth muscle cellsCell proliferationExtracellular signal-regulated protein kinases 1Mitogen-activated protein kinaseSmooth muscle cell proliferationProtein kinase 1Smooth muscle cellsMuscle cell proliferationMAP kinase activationDegree of phosphorylationL-homocysteineMuscle cellsProtein kinaseKinase studiesKinase activationKinase 1Hallmark of atherosclerosisKinasePhosphorylationActive formTranslocation of PKC isoforms in bovine aortic smooth muscle cells exposed to strain
Han O, Takei T, Basson M, Sumpio B. Translocation of PKC isoforms in bovine aortic smooth muscle cells exposed to strain. Journal Of Cellular Biochemistry 2000, 80: 367-372. PMID: 11135367, DOI: 10.1002/1097-4644(20010301)80:3<367::aid-jcb100>3.0.co;2-2.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsAortic smooth muscle cellsBovine aortic smooth muscle cellsMuscle cellsPKC-alphaPKC isoformsCyclic strain resultsCycles/minCytosolic fractionProliferation responseParticulate fractionNF-kappaBProtein kinase C activityCollagen type ICellular proliferationSignificant stimulationImmunoblotting analysisKinase C activityType IPKC activityC activityStimulationIndividual PKC isoformsIsoforms
1998
Coculture conditions alter endothelial modulation of TGF-β1 activation and smooth muscle growth morphology
Powell R, Bhargava J, Basson M, Sumpio B. Coculture conditions alter endothelial modulation of TGF-β1 activation and smooth muscle growth morphology. American Journal Of Physiology 1998, 274: h642-h649. PMID: 9486269, DOI: 10.1152/ajpheart.1998.274.2.h642.Peer-Reviewed Original Research
1997
Effect of pressure on cultured smooth muscle cells
Watase M, Awolesi M, Ricotta J, Sumpio B. Effect of pressure on cultured smooth muscle cells. Life Sciences 1997, 61: 987-996. PMID: 9296337, DOI: 10.1016/s0024-3205(97)00603-6.Peer-Reviewed Original Research
1994
Chronic Cyclic Strain Reduces Adenylate Cyclase Activity and Stimulatory G Protein Subunit Levels in Coronary Smooth Muscle Cells
Wiersbitzky M, Mills I, Sumpio B, Gewirtz H. Chronic Cyclic Strain Reduces Adenylate Cyclase Activity and Stimulatory G Protein Subunit Levels in Coronary Smooth Muscle Cells. Experimental Cell Research 1994, 210: 52-55. PMID: 8269996, DOI: 10.1006/excr.1994.1008.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsSmooth muscle cellsAdenylate cyclase activityCoronary vascular smooth muscle cellsMuscle cellsCyclase activityChronic cyclic strainG protein subunit levelsCoronary smooth muscle cellsProtein steady-state levelsCoronary smooth muscleCultured smooth muscle cellsSteady-state levelsPorcine coronary arteriesCoronary arteryCoronary circulationBeta levelsCycles/minProtein subunit levelsSmooth muscleGi alphaIntravascular pressureEffector pathwaysUnstretched cellsSubunit levels
1993
THE EXCITATION OF 8‐METHOXYPSORALEN WITH VISIBLE LIGHT: REVERSED PHASE HPLC QUANTITATION OF MONOADDUCTS and CROSS‐LINKS
Gasparro F, Gattolin P, Olack G, Deckelbaum L, Sumpio B. THE EXCITATION OF 8‐METHOXYPSORALEN WITH VISIBLE LIGHT: REVERSED PHASE HPLC QUANTITATION OF MONOADDUCTS and CROSS‐LINKS. Photochemistry And Photobiology 1993, 57: 1007-1009. PMID: 8367529, DOI: 10.1111/j.1751-1097.1993.tb02963.x.Peer-Reviewed Original Research