2007
Effect of different frequencies of tensile strain on human dermal fibroblast proliferation and survival
Nishimura K, Blume P, Ohgi S, Sumpio BE. Effect of different frequencies of tensile strain on human dermal fibroblast proliferation and survival. Wound Repair And Regeneration 2007, 15: 646-656. PMID: 17971010, DOI: 10.1111/j.1524-475x.2007.00295.x.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinaseP38 mitogen-activated protein kinaseMAPK/ERK kinaseExtracellular signal-regulated kinaseDominant-negative AktHuman dermal fibroblastsSignal-regulated kinaseCell numberERK kinaseProtein kinaseTransduction pathwaysDermal fibroblastsRelevant transduction pathwaysRelevant signal pathwaysDermal fibroblast proliferationHuman dermal fibroblast proliferationCultured human dermal fibroblastsSurvival of fibroblastsSignal pathwayKinaseNecrotic fibroblastsDNA synthesisCell proliferationAktTransferase-mediated dUTP nick-end labeling staining
2006
Nicotine enhances human vascular endothelial cell expression of ICAM-1 and VCAM-1 via protein kinase C, p38 mitogen-activated protein kinase, NF-κB, and AP-1
Ueno H, Pradhan S, Schlessel D, Hirasawa H, Sumpio BE. Nicotine enhances human vascular endothelial cell expression of ICAM-1 and VCAM-1 via protein kinase C, p38 mitogen-activated protein kinase, NF-κB, and AP-1. Cardiovascular Toxicology 2006, 6: 39-50. PMID: 16845181, DOI: 10.1385/ct:6:1:39.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsICAM-1VCAM-1NF-κBAdhesion moleculesP38 mitogen-activated protein kinaseProtein kinase CMitogen-activated protein kinaseVascular cell adhesion moleculeEndothelial cellsExposure of HUVECsCellular adhesion molecule expressionAP-1Vascular endothelial cell expressionMajor risk factorEffects of nicotineEtiology of atherosclerosisP38 MAPKAdhesion molecule expressionIntercellular adhesion moleculeTranscription factor NF-κBCellular adhesion moleculesEndothelial cell expressionFactor NF-κBUmbilical vein endothelial cells
2005
Nicotine induces mitogen-activated protein kinase dependent vascular smooth muscle cell migration
Di Luozzo G, Pradhan S, Dhadwal AK, Chen A, Ueno H, Sumpio BE. Nicotine induces mitogen-activated protein kinase dependent vascular smooth muscle cell migration. Atherosclerosis 2005, 178: 271-277. PMID: 15694934, DOI: 10.1016/j.atherosclerosis.2004.09.017.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cell migrationSmooth muscle cell migrationMuscle cell migrationInhibitors of p38VSMC migrationMitogen-activated protein kinaseEndothelial cellsDevelopment of atherosclerosisEffects of nicotineCell migrationWestern blotting methodInjury modelVascular endotheliumCigarette smokeBoyden chamber chemotaxis assaysAortic VSMCsChemotactic effectMAPKs p38NicotineChemoattractant moleculesChemotaxis assaysCellular mechanismsUltrastructural changesVSMCP44/42 activation
2004
Effects of Cyclic Strain on Vascular Cells
Kakisis JD, Liapis CD, Sumpio BE. Effects of Cyclic Strain on Vascular Cells. Endothelium 2004, 11: 17-28. PMID: 15203876, DOI: 10.1080/10623320490432452.Peer-Reviewed Original ResearchConceptsCAMP-responsive elementVascular cell morphologyExtracellular mechanical signalsMitogen-activated protein kinaseProtein kinase CPlatelet-derived growth factor receptorEarly growth responseCells senseNumerous genesActivator proteinTranscription factorsProtein kinaseGrowth factor receptorAP-2Chemical signalsSecond messengerKinase CG proteinsMechanical signalsCell nucleiGrowth responseIon channelsCell morphologyFactor receptorCell proliferation
2003
Antisense basic fibroblast growth factor alters the time course of mitogen-activated protein kinase in arterialized vein graft remodeling
Yamashita A, Hanna AK, Hirata S, Dardik A, Sumpio BE. Antisense basic fibroblast growth factor alters the time course of mitogen-activated protein kinase in arterialized vein graft remodeling. Journal Of Vascular Surgery 2003, 37: 866-873. PMID: 12663990, DOI: 10.1067/mva.2003.130.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood Vessel ProsthesisCell Physiological PhenomenaEndothelium, VascularFibroblast Growth FactorsHyperplasiaJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein KinasesModels, AnimalP38 Mitogen-Activated Protein KinasesProteinsRabbitsRegenerationRNA, AntisenseTime FactorsTransfectionVeinsConceptsProtein kinaseBasic fibroblast growth factorMAPK activationFibroblast growth factorMitogen-activated protein kinase (MAPK) familyC-Jun N-terminal protein kinaseProtein kinase familyExtracellular signal-regulated kinase 1/2Antisense basic fibroblast growth factorMitogen-activated protein kinaseSignal-regulated kinase 1/2Messenger RNA sequencesP38 kinase activationGrowth factor altersMAPK-dependent mechanismFibroblast growth factor activityGrowth factorKinase familyKinase activationGrowth factor activityPhosphorylation of MAPKsKinase 1/2RNA sequencesWestern blot analysisBasic fibroblast growth factor activity2P-0488 Nicotine enhances endothelial cell (EC) adhesion molecule expression — Involvement of protein kinase C (PKC), P38 mitogen-activated protein kinase (MAPK) and NFkB
Ueno H, Pradhan S, Wang X, Schlessel D, Hirasawa H, Sumpio B. 2P-0488 Nicotine enhances endothelial cell (EC) adhesion molecule expression — Involvement of protein kinase C (PKC), P38 mitogen-activated protein kinase (MAPK) and NFkB. Atherosclerosis Plus 2003, 4: 147. DOI: 10.1016/s1567-5688(03)90629-5.Peer-Reviewed Original Research
2002
SPHINGOSINE-1-PHOSPHATE STIMULATES HUMAN CACO-2 INTESTINAL EPITHELIAL PROLIFERATION VIA p38 ACTIVATION AND ACTIVATES ERK BY AN INDEPENDENT MECHANISM
THAMILSELVAN V, LI W, SUMPIO BE, BASSON MD. SPHINGOSINE-1-PHOSPHATE STIMULATES HUMAN CACO-2 INTESTINAL EPITHELIAL PROLIFERATION VIA p38 ACTIVATION AND ACTIVATES ERK BY AN INDEPENDENT MECHANISM. In Vitro Cellular & Developmental Biology - Animal 2002, 38: 246-253. PMID: 12197778, DOI: 10.1290/1071-2690(2002)038<0246:spshci>2.0.co;2.Peer-Reviewed Original ResearchConceptsExtracellular signal-regulated kinases 1Mitogen-activated protein kinaseMAP kinase kinaseCaco-2 proliferationMAPK activationHuman intestinal epithelial proliferationP38 activationCell typesSignal-regulated kinases 1Role of ERKMitogenic effectCaco-2 intestinal epithelial cellsIntracellular second messengerMEK inhibitionP38 MAPK activationCancer cell invasionKinase kinaseHuman Caco-2 intestinal epithelial cellsProtein kinaseStimulation of proliferationCell motilityIntestinal epithelial cell proliferationInhibitor PD98059ERK2ERK activation
2000
Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain
Kito H, Chen E, Wang X, Ikeda M, Azuma N, Nakajima N, Gahtan V, Sumpio B. Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain. Journal Of Applied Physiology 2000, 89: 2391-2400. PMID: 11090594, DOI: 10.1152/jappl.2000.89.6.2391.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell MovementCells, CulturedEndothelium, VascularEnzyme ActivationEnzyme InhibitorsFlavonoidsImidazolesJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein KinasesP38 Mitogen-Activated Protein KinasesPhosphorylationPromoter Regions, GeneticPulmonary ArteryPyridinesResponse ElementsStress, MechanicalTetradecanoylphorbol AcetateTranscription Factor AP-1ConceptsPD 98059Activator protein-1SB 203580Protein kinaseBovine pulmonary arterial endothelial cellsPulmonary arterial endothelial cellsArterial endothelial cellsMAPK kinase kinase-1Mitogen-activated protein kinase activationMitogen-activated protein kinaseExtracellular signal-regulated kinaseTerminal protein kinaseKinase kinase 1AP-1/Cell alignmentERK kinase inhibitorProtein kinase activationSignal-regulated kinaseEndothelial cellsTranscriptional activationInactive mutantActivated MAPKsKinase activationKinase 1Transient transfectionHomocysteine stimulates MAP kinase in bovine aortic smooth muscle cells
Woo D, Dudrick S, Sumpio B. Homocysteine stimulates MAP kinase in bovine aortic smooth muscle cells. Surgery 2000, 128: 59-66. PMID: 10876187, DOI: 10.1067/msy.2000.106531.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDose-Response Relationship, DrugEnzyme ActivationEnzyme InhibitorsFlavonoidsHomocysteineMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMuscle, Smooth, VascularPhosphorylationConceptsMAP kinase phosphorylationMAP kinaseKinase phosphorylationBASMC proliferationBovine aortic smooth muscle cellsAortic smooth muscle cellsCell proliferationExtracellular signal-regulated protein kinases 1Mitogen-activated protein kinaseSmooth muscle cell proliferationProtein kinase 1Smooth muscle cellsMuscle cell proliferationMAP kinase activationDegree of phosphorylationL-homocysteineMuscle cellsProtein kinaseKinase studiesKinase activationKinase 1Hallmark of atherosclerosisKinasePhosphorylationActive formMitogen‐activated protein phosphorylation in endothelial cells exposed to hyperosmolar conditions
Duzgun S, Rasque H, Kito H, Azuma N, Li W, Basson M, Gahtan V, Dudrick S, Sumpio B. Mitogen‐activated protein phosphorylation in endothelial cells exposed to hyperosmolar conditions. Journal Of Cellular Biochemistry 2000, 76: 567-571. PMID: 10653976, DOI: 10.1002/(sici)1097-4644(20000315)76:4<567::aid-jcb5>3.0.co;2-w.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein KinasesCattleCell SizeEndothelium, VascularGlucoseJNK Mitogen-Activated Protein KinasesMannitolMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesOsmolar ConcentrationOsmotic PressureP38 Mitogen-Activated Protein KinasesPhosphorylationSignal TransductionSodium ChlorideTime FactorsUrea